Vitamin-B12-conjugated PLGA-PEG nanoparticles incorporating miR-532-3p induce mitochondrial damage by targeting apoptosis repressor with caspase recruitment domain (ARC) on CD320-overexpressed gastric cancer

Chen, Zhian; Liang, Yanrui; Feng, Xiaoli; Yu, Liang; Shen, Guodong; Huang, Huilin; Chen, Zhaoyu; Yu, Jiang; Liu, Hao; Lin, Tian; Hao, Chunyan; Wu, Dong; Li, Guoxin; Zhao, Bingxia; Guo, Weihong; Hu, Yanfeng

doi:10.1016/j.msec.2020.111722

Cited by 28 publications

(21 citation statements)

References 56 publications

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“…(miR-532-3p@PLGA-PEG-VB12 NPs) were developed as a targeting gene delivery system to be selectively delivered into transcobalamin IIoverexpressed gastric cancer cells. 81 Rapidly dividing cells, such as tumor cells, have high vitamin B12 requirement and its receptor (the transcobalamin II receptor, CD320) is overexpressed in many cancer cells. The miR-532-3p@PLGA-PEG-VB12 NPs significantly decreased the expression of apoptosis repressor with caspase recruitment domain (ARC), inducing activation of the ARC/Bax/mitochondria-mediated apoptosis signaling pathway, that finally suppressed proliferation of gastric cancer cells both in vitro and in vivo.…”

Section: Dovepressmentioning

confidence: 99%

“…The miR-532-3p@PLGA-PEG-VB12 NPs significantly decreased the expression of apoptosis repressor with caspase recruitment domain (ARC), inducing activation of the ARC/Bax/mitochondria-mediated apoptosis signaling pathway, that finally suppressed proliferation of gastric cancer cells both in vitro and in vivo. 81 Hyaluronic acid-and chondroitin sulfate-modified PLGA-PEG copolymers were synthesized and employed to prepare pH-responsive NPs loaded with DOTAP/pDNA lipoplex. 82 Both types of NPs owned targeting moiety to specifically bind CD44 receptors, resulting in efficient uptake and higher transfection in CD44 high-expressed…”

Section: Dovepressmentioning

confidence: 99%

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Recent Advances and Challenges in Gene Delivery Mediated by Polyester-Based Nanoparticles

Piperno¹,

Sciortino²,

Giusto³

et al. 2021

IJN

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Gene therapy is a promising approach for the treatment of several diseases, such as chronic or viral infections, inherited disorders, and cancer. The cellular internalization of exogenous nucleic acids (NA) requires efficient delivery vehicles to overcome their inherent pharmacokinetic drawbacks, e.g. electrostatic repulsions, enzymatic degradation, limited cellular uptake, fast clearance, etc. Nanotechnological advancements have enabled the use of polymer-based nanostructured biomaterials as safe and effective gene delivery systems, in addition to viral vector delivery methods. Among the plethora of polymeric nanoparticles (NPs), this review will provide a comprehensive and in-depth summary of the polyesterbased nanovehicles, including poly(lactic-co-glycolic acid) (PLGA) and polylactic acid (PLA) NPs, used to deliver a variety of foreign NA, e.g. short interfering RNA (siRNA), messenger RNA (mRNA), and plasmid DNA (pDNA). The article will review the versatility of polyester-based nanocarriers including their recent application in the delivery of the clustered, regularly-interspaced, short palindromic repeats/Cas (CRISPR/Cas) genome editing system for treating gene-related diseases. The remaining challenges and future trend of the targeted delivery of this revolutionary genome-editing system will be discussed. Special attention will be given to the pivotal role of nanotechnology in tackling emerging infections such as coronavirus disease 2019 (COVID-19): ground-breaking mRNA vaccines delivered by NPs are currently used worldwide to fight the pandemic, pushing the boundaries of gene therapy.

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Section: Dovepressmentioning

confidence: 99%

Section: Dovepressmentioning

confidence: 99%

Recent Advances and Challenges in Gene Delivery Mediated by Polyester-Based Nanoparticles

Piperno¹,

Sciortino²,

Giusto³

et al. 2021

IJN

View full text Add to dashboard Cite

show abstract

“…Following mPTP opening, water and solutes will flow freely into the mitochondrial matrix, leading to mitochondrial swelling, loss of the mitochondrial membrane potential, and release of intermembrane space proteins (e.g., cytochrome c and apoptosisinducing factor). Eventually, caspase-dependent or caspaseindependent cell death will occur depending on the overall severity of mitochondrial dysfunction that has occurred within the individual myocyte [28,29].…”

Section: Mitochondrial Oxidative Stress and Mptp Openingmentioning

confidence: 99%

Irisin: A Promising Target for Ischemia‐Reperfusion Injury Therapy

Wang

Liu

Sun

et al. 2021

Oxidative Medicine and Cellular Longevity

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Ischemia-reperfusion injury (IRI) is defined as the total combined damage that occurs during a period of ischemia and following the recovery of blood flow. Oxidative stress, mitochondrial dysfunction, and an inflammatory response are factors contributing to IRI-related damage that can each result in cell death. Irisin is a polypeptide that is proteolytically cleaved from the extracellular domain of fibronectin type III domain-containing protein 5 (FNDC5). Irisin acts as a myokine that potentially mediates beneficial effects of exercise by reducing oxidative stress, improving mitochondrial fitness, and suppressing inflammation. The existing literature also suggests a possible link between irisin and IRI, involving mechanisms similar to those associated with exercise. This article will review the pathogenesis of IRI and the potential benefits and current limitations of irisin as a therapeutic strategy for IRI, while highlighting the mechanistic correlations between irisin and IRI.

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“…Most studies conducted using vitamin B12 have proven that when it is used in combined therapy it contributes to increasing the effectiveness of anticancer drugs. As a new promising therapeutic application, vitamin B12-conjugates such as vitamin B12-labeled poly(D,L-lactide-co-glycolide) and polyethylene glycol nanoparticles (PLGA-PEG-VB12 NPs), have been used with microRNA system in the form of miR-532-3p-loaded PLGA-PEG nanoparticles as an anti-proliferative agent for gastric cancer via activating the ARC/Bax/mitochondria-mediated apoptosis signaling pathway [34]. Additionally, it was shown that vitamin B12 contributes to a distinctive mechanism of cell death known as paraptosis-like cell death.…”

Section: Introductionmentioning

confidence: 99%

Vitamin B12 enhances the antitumor activity of 1,25-dihydroxyvitamin D3 via activation of caspases and targeting actin cytoskeleton

Atoum

Alzoughool

Al-Mazaydeh

et al. 2022

TUB

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BACKGROUND: 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) is an effective anticancer agent, and when combined with other agents it shows superior activities. Vitamin B12 has been shown to contribute to increasing the effectiveness of anticancer drugs when used in combination. Thus, the current study aimed at investigating the anticancer potential of the combination of 1,25(OH)2D3 and vitamin B12. METHODS: MTT assay was used to determine the cytotoxic activity of combining 1,25(OH)2D3 and vitamin B12 against six different cancer cell lines and one normal cell line. The surviving fraction after clonogenic assay was measured, and the effects of 1,25(OH)2D3/B12 combination on the activity of different caspases, cell adhesion, actin cytoskeleton, cell morphology, and percentage of polarized cells were evaluated. RESULTS: Vitamin B12 did not cause cytotoxicity, however, it enhanced the cytotoxicity of 1,25(OH)2D3 against cancer cells. The cytotoxic effects of 1,25(OH)2D3 and its combination with vitamin B12 was not evident in the normal mammary MCF10A cell line indicating cancer cell-specificity. The cytotoxic effects of 1,25(OH)2D3/B12 combination occurred in a dose-dependent manner and was attributed to apoptosis induction which was mediated by caspase 4 and 8. Moreover, 1,25(OH)2D3/B12-treated cells showed enhanced inhibition of clonogenic tumor growth, reduced cell adhesion, reduced cell area, reduced percentage of cell polarization, and disorganized actin cytoskeleton resulting in reduced migratory phenotype when compared to cells treated with 1,25(OH)2D3 alone. CONCLUSION: 1,25(OH)2D3 and vitamin B12 exhibited synergistic anticancer effects against different cancer cell lines. The combination therapy of 1,25(OH)2D3 and vitamin B12 may provide a potential adjunctive treatment option for some cancer types.

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Vitamin-B12-conjugated PLGA-PEG nanoparticles incorporating miR-532-3p induce mitochondrial damage by targeting apoptosis repressor with caspase recruitment domain (ARC) on CD320-overexpressed gastric cancer

Cited by 28 publications

References 56 publications

Recent Advances and Challenges in Gene Delivery Mediated by Polyester-Based Nanoparticles

Recent Advances and Challenges in Gene Delivery Mediated by Polyester-Based Nanoparticles

Irisin: A Promising Target for Ischemia‐Reperfusion Injury Therapy

Vitamin B12 enhances the antitumor activity of 1,25-dihydroxyvitamin D3 via activation of caspases and targeting actin cytoskeleton

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