Vitamin B1 and B6 method harmonization: Comparison of performance between laboratories enrolled in the RCPA Quality Assurance Program

Thiamine is an essential micronutrient that plays a key role in energy metabolism. Many populations worldwide may be at risk of clinical or subclinical thiamine deficiencies, due to famine, reliance on staple crops with low thiamine content, or food preparation practices, such as milling grains and washing milled rice. Clinical manifestations of thiamine deficiency are variable; this, along with the lack of a readily accessible and widely agreed upon biomarker of thiamine status, complicates efforts to diagnose thiamine deficiency and assess its global prevalence. Strategies to identify regions at risk of thiamine deficiency through proxy measures, such as analysis of food balance sheet data and month-specific infant mortality rates, may be valuable for understanding the scope of thiamine deficiency. Urgent public health responses are warranted in high-risk regions, considering the contribution of thiamine deficiency to infant mortality and research suggesting that even subclinical thiamine deficiency in childhood may have lifelong neurodevelopmental consequences. Food fortification and maternal and/or infant thiamine supplementation have proven effective in raising thiamine status and reducing the incidence of infantile beriberi in regions where thiamine deficiency is prevalent, but trial data are limited. Efforts to determine culturally and environmentally appropriate food vehicles for thiamine fortification are ongoing.

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“…Analytical methods have not been standardized. Considerable variation has been observed between laboratories …”

Section: Biomarkers For Assessing Thiamine Statusmentioning

confidence: 99%

Thiamine deficiency disorders: diagnosis, prevalence, and a roadmap for global control programs

Whitfield

Bourassa

Adamolekun

et al. 2018

Annals of the New York Academy of Sciences

228

335

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“…Whether this is attributed to the company's own decision from published information or from consumer demand to improve accuracy and precision is not known [22]. What can be acknowledged is that this is a necessity to improve the performance of these quantitative methods.…”

Section: Quality Goalsmentioning

confidence: 99%

“…A survey of participants enrolled in the RCPAQAP whole-blood programme revealed that only one of nine respondents included an internal standard [22]. Furthermore, from our literature search, only four publications reported the use of an internal standard [109,125,128,129].…”

Section: Quality Goalsmentioning

confidence: 99%

“…To turn the numerical value into a clinically meaningful result we need an appropriate decision point(s) such as a reference interval. A survey of RCPAQAP participants measuring vitamins B1, conducted in 2012, found a general consensus towards reported reference intervals for vitamin B1 [22]. The survey concluded that the apparent harmonisation of reference intervals was based upon their adoption from the reagent kit inserts, as 66% of the responding participants utilised a common commercially available reagent.…”

Section: Post-analyticalmentioning

confidence: 99%

“…The RCPAQAP and SKML schemes consist of a sample set of six linearly related levels analysed twice per cycle with two cycles each year [22,23]. All participants are presented with a survey to classify each component of their method.…”

Section: Eqa Programmesmentioning

confidence: 99%

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Vitamin B1 in critically ill patients: needs and challenges

Collie

Greaves

Jones

et al. 2017

Clinical Chemistry and Laboratory Medicine (CCLM)

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Background: Thiamine has a crucial role in energy production, and consequently thiamine deficiency (TD) has been associated with cardiac failure, neurological disorders, oxidative stress (lactic acidosis and sepsis) and refeeding syndrome (RFS). This review aims to explore analytical methodologies of thiamine compound quantification and highlight similarities, variances and limitations of current techniques and how they may be relevant to patients. Content: An electronic search of Medline, PubMed and Embase databases for original articles published in peerreviewed journals was conducted. MethodsNow was used to search for published analytical methods of thiamine compounds. Keywords for all databases included "thiamine and its phosphate esters", "thiamine methodology" and terms related to critical illness. Enquiries were also made to six external quality assurance (EQA) programme organisations for the inclusion of thiamine measurement.

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Methods for assessment of Thiamine (Vitamin B1)

Crook

2019

Laboratory Assessment of Vitamin Status

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Vitamin B1 and B6 method harmonization: Comparison of performance between laboratories enrolled in the RCPA Quality Assurance Program

Cited by 18 publications

References 11 publications

Thiamine deficiency disorders: diagnosis, prevalence, and a roadmap for global control programs

Thiamine deficiency disorders: diagnosis, prevalence, and a roadmap for global control programs

Vitamin B1 in critically ill patients: needs and challenges

Methods for assessment of Thiamine (Vitamin B1)

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