Vitamin B and Vitamin C Affect DNA Methylation and Amino Acid Metabolism in Mycobacterium bovis BCG

Song, Ningning; Zhu, Yaping; Cui, Yingying; Lv, Mingyue; Tang, Yiyi; Cui, Ziyin; Dang, Guanghui; Zheng, Huajun; Liu, Siguo

doi:10.3389/fmicb.2020.00812

Cited by 6 publications

(9 citation statements)

References 67 publications

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“…Recently, scholars 38 have found that VB6 is related to the metabolism of H 2 S, H 2 S 2 , cystine, cysteine, homocysteine, cystine sulfide, and methionine in the metabolic reprogramming of liver cancer. 39 Many clinical trials 40–42 have observed an anti-hepatocellular carcinoma effect by directly supplementing VB6, and some studies reported negative results. In the present study, we constructed a mouse model of chronic liver injury by CCl4 and treated these mice with VB6.…”

Section: Discussionmentioning

confidence: 99%

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Vitamin B6 Metabolic Pathway is Involved in the Pathogenesis of Liver Diseases via Multi-Omics Analysis

Mei¹,

Liu²,

Tang³

et al. 2022

JHC

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Purpose To clarify the underlying regulatory mechanisms of progression from liver cirrhosis to hepatocellular carcinoma (HCC), we analyzed the microbiomics, metabolomics, and proteomics in plasma and tissues from patients with HCC or decompensated liver cirrhosis (DC). Patients and Methods Tissues and plasma from 44 HCC patients and 28 patients with DC were collected for metabolomic analysis. 16S rRNA sequencing was performed in nine HCC tissues (HCCT), four distal noncancerous tissues (HCCN), and 11 DC tissues (DCT). Five HCC tissues had liver cirrhosis (HCCT-LC). Five hepatocellular carcinoma tissues without liver cirrhosis (HCCT-NLC) and five DCT were selected for proteomic sequencing. After combining proteomic and metabolomic analysis, we constructed a mouse model of chronic liver injury using carbon tetrachloride (CCl4) and treated them with vitamin B6 (VB6). Results 16s rRNA sequence results showed that HCC tissues had higher alpha diversity. The highest LDA scores were detected for Elizabethkingia in HCCT, Subsaxibacter in DCT, and Stenotrophomon in HCCN. Metabolomics results demonstrated some metabolites, including capric acid, L-threonate, choline, alpha-D-Glucose, D-ribose, betaine, 2E-eicosenoic acid, linoleic acid, L-palmitoylcarnitine, taurodeoxycholic acid, L-pyroglutamic acid, androsterone sulfate, and phthalic acid mono-2-ethylhexyl ester (MEHP), had better diagnostic efficacy than AFP (AUC: 0.852; 95% CI: 0.749, 0.954). In a combined analysis of metabolomics and proteomics, we found that HCCT-LC had more obvious disorders of VB6 metabolism and pentose and glucuronate interconversions than DCT, and kynurenine metabolism disorder was more significant in HCCT-LC than in HCCT-NLC. In the CCl4-induced chronic liver injury model, after VB6 supplementation, inflammatory cell infiltration, hepatocyte edema, and degeneration were significantly improved. Conclusion We found significant differences in the flora distribution between HCCT and DC; MEHP was a new diagnostic biomarker of HCC, and VB6 ameliorated the inflammatory cell infiltration, hepatocyte edema, and degeneration in chronic liver injury.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Vitamin B6 Metabolic Pathway is Involved in the Pathogenesis of Liver Diseases via Multi-Omics Analysis

Mei¹,

Liu²,

Tang³

et al. 2022

JHC

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“…Iron had a similar but insignificant effect. The reason could be that Zn is an inorganic element, which can activate enzyme activity (C. Li, 2015), and amino acids are organic substances, with their metabolic pathways also affected by other organic substances such as vitamins (N. Song, Zhu, et al., 2020). Zinc may combine with the functional groups of amino acids, destroy the molecular structure of amino acids, and affect the chemical processes such as hydrolysis.…”

Section: Discussionmentioning

confidence: 99%

Management practices effects on Zanthoxylum planispinum ‘dintanensis’ fruit quality

Song

2022

Agronomy Journal

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In this work, field control experiments were conducted to clarify the effects of three management practices (dwarf and close planting + no sprinkling irrigation (A1), dwarf and close planting + sprinkling irrigation (A2), and natural growth (CK) on the quality of pericarp of Zanthoxylum planispinum ‘dintanensis’ in a plantation setting in a rocky desertification area of Central Guizhou Province. The results showed that the ash concentration of A2 was the highest (6.70% ± 0.42%), yet there was no significant difference from A1 (6.60% ± 0.14%) and CK (6.65% ± 0.64%), and methionine and cystine were not detected (detection limits were 13.90 and 43.60 mg kg–1, respectively). A2 management could significantly promote the concentration of amino acids (P < .05); vitamin C was not detected (detection limit was 0.20 mg kg–1), vitamin E ranked as A2 > A1 > CK, and β‐carotene ranked as A1 > A2 > CK. There were significant differences among management treatments; A1, A2, and CK had no significant effect on trace elements. In conclusion, dwarf and close planting + sprinkling irrigation is an ideal management practice improving the quality of dintanensis.

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“…However, with advancement of microbial genomics, it may be possible to utilize vitamins to target DNA/RNA so as to prevent growth and cause death 65,66 . Example, vitamins B and C can interfere with the normal DNA methylation causing reduced thermodynamic stability and expression of the restriction modification system of Mycobacterium bovis (BCG) 67 . Vitamin B 12 serve as a nonpeptidic carrier for peptide nucleic acid to target the messenger RNA so as to inhibit gene expression in E. coli 68 .…”

Section: Vitamins and Genomic Influence On Resistant Microbial Agentsmentioning

confidence: 99%

The dawn of repurposing vitamins as potential novel antimicrobial agents: A call for global emergency response amidst AMR crisis

Max

Mashauri

2023

Health Science Reports

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Amidst, the global pandemic of antimicrobial resistance (AMR), the rate at which AMR increases overwhelms the increased efforts to discover new effective antimicrobials. There is a persistent need for alternative treatment modalities so as to keep up with the pace. AMR is the leading cause of death in the world and its health and economic consequences suggest the urgent need for sustainable interventions. Vitamins have consistently proven to have antimicrobial activity as well as slowing down the AMR rate by influencing the AMR genes even towards extensive multidrug resistant strains. Evidences suggest that the use of some vitamins on their own or in combination with existing antimicrobial agents could be a breakthrough towards combating AMR. This will widen the antimicrobial agents' options in the treatment arena, preserve the antimicrobial agents susceptible to develop resistant so that they can be used in severe infections only, reduce the tension and burden of the AMR crisis significantly and give enough room for development of new antimicrobial agents. Moreover, almost all viral, fungal, parasitic and bacterial resistant strains of concern as listed by World Health Organization have been found to be sensitive to several vitamins either synergistically with other antimicrobials or independently. Considering their widened spectrum of immunomodulatory and antimicrobial effect, some vitamins can further be repositioned as prophylactic antimicrobial agents in clinical situations like in presurgeries prophylaxis so as to avoid unnecessary use of antimicrobials especially antibiotics. Various relevant AMR stakeholders should invest in clinical trials and systematic reviews with available data to enable quick repositioning of some potential vitamins as antimicrobial agents as an emergency rapid response towards AMR Crisis. This includes the preparation of guidelines containing specificity of which vitamin to be used for treatment of which type of infection.

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Vitamin B and Vitamin C Affect DNA Methylation and Amino Acid Metabolism in Mycobacterium bovis BCG

Cited by 6 publications

References 67 publications

Vitamin B6 Metabolic Pathway is Involved in the Pathogenesis of Liver Diseases via Multi-Omics Analysis

Vitamin B6 Metabolic Pathway is Involved in the Pathogenesis of Liver Diseases via Multi-Omics Analysis

Management practices effects on Zanthoxylum planispinum ‘dintanensis’ fruit quality

The dawn of repurposing vitamins as potential novel antimicrobial agents: A call for global emergency response amidst AMR crisis

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