Vitamin A deficiency impairs intestinal physical barrier function of fish

Jiang, Wei‐Dan; Zhou, Xiao‐Qiu; Zhang, Li; Liu, Yang; Wu, Pei; Jiang, Jun; Kuang, Sheng‐Yao; Tang, Ling; Tang, Wu‐Neng; Zhang, Yongan; Shi, He-Qun; Feng, Lin

doi:10.1016/j.fsi.2019.01.056

Cited by 40 publications

(17 citation statements)

References 69 publications

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“…Different dietary interventions have variable effects on fish intestinal TJs. Vitamin A deficiency decreased the mRNA levels of TJ complexes (several cldns and tjp1 ) in grass carp ( Ctenopharyngodon idella ) [55], dietary isoleucine decreased the expression of several cldns in Jian carp ( Cyprinus carpio var. Jian) [56], dietary deoxynivalenol (a mycotoxin) also decreased the relative expression of markers for three TJ proteins in Atlantic salmon ( Salmo salar ) intestine [57], and some plant proteins induced significant alterations of the TJ signalling pathway in this same species [11].…”

Section: Discussionmentioning

confidence: 99%

“…In addition, under biotin-deficient conditions, innate immune system cells produce increased levels of pro-inflammatory cytokines and Th1- and Th17-mediated proinflammatory responses in human CD4+ T lymphocytes [85]. Furthermore, both deficiency and excess of dietary pantothenic acid downregulate several cldns , occludin and tjp1 mRNA levels in all intestinal segments of grass carp [86], and dietary deficiency of another vitamin (vitamin A) also impaired physical barrier functions associated with impaired antioxidant capacity, aggravated cell apoptosis and disrupted TJ complexes in the intestine of grass carp [55]. In contrast, another vitamin related compound, para-aminobenzoic acid (PABA), was increased in parasitized fish.…”

Section: Discussionmentioning

confidence: 99%

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Disruption of gut integrity and permeability contributes to enteritis in a fish-parasite model: a story told from serum metabolomics

et al. 2019

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Background In the animal production sector, enteritis is responsible for serious economic losses, and intestinal parasitism is a major stress factor leading to malnutrition and lowered performance and animal production efficiency. The effect of enteric parasites on the gut function of teleost fish, which represent the most ancient bony vertebrates, is far from being understood. The intestinal myxozoan parasite Enteromyxum leei dwells between gut epithelial cells and causes severe enteritis in gilthead sea bream (Sparus aurata), anorexia, cachexia, growth impairment, reduced marketability and increased mortality. Methods This study aimed to outline the gut failure in this fish-parasite model using a multifaceted approach and to find and validate non-lethal serum markers of gut barrier dysfunction. Intestinal integrity was studied in parasitized and non-parasitized fish by immunohistochemistry with specific markers for cellular adhesion (E-cadherin) and tight junctions (Tjp1 and Cldn3) and by functional studies of permeability (oral administration of FITC-dextran) and electrophysiology (Ussing chambers). Serum samples from parasitized and non-parasitized fish were analyzed using non-targeted metabolomics and some significantly altered metabolites were selected to be validated using commercial kits. Results The immunodetection of Tjp1 and Cldn3 was significantly lower in the intestine of parasitized fish, while no strong differences were found in E-cadherin. Parasitized fish showed a significant increase in paracellular uptake measured by FITC-dextran detection in serum. Electrophysiology showed a decrease in transepithelial resistance in infected animals, which showed a diarrheic profile. Serum metabolomics revealed 3702 ions, from which the differential expression of 20 identified compounds significantly separated control from infected groups in multivariate analyses. Of these compounds, serum inosine (decreased) and creatine (increased) were identified as relevant and validated with commercial kits. Conclusions The results demonstrate the disruption of tight junctions and the loss of gut barrier function, a metabolomic profile of absorption dysfunction and anorexia, which further outline the pathophysiological effects of E. leei.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Disruption of gut integrity and permeability contributes to enteritis in a fish-parasite model: a story told from serum metabolomics

et al. 2019

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“…Vitamin A, which is an unsaturated monohydric alcohol with β-ionone ring, is an essential nutrient for fish and capable of scavenging peroxyl radicals thus inhibiting lipid peroxidation in vitro [ 1 ]. Our previous study showed that vitamin A reduced the oxidative damage of lipid and protein in grass carp intestine [ 2 ]. To our knowledge, fish is an important animal-source food providing essential nutrients with high bioavailability, such as balanced amino acid and omega 3 long-chain polyunsaturated fatty acids (n-3 LC PUFA) for humans [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Dietary Vitamin A Improved the Flesh Quality of Grass Carp (Ctenopharyngodon idella) in Relation to the Enhanced Antioxidant Capacity through Nrf2/Keap 1a Signaling Pathway

Zhang

Jiang

et al. 2022

Antioxidants

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Fish is an important animal-source food for humans. However, the oxidative stress-induced by intensive aquaculture usually causes deterioration of fish meat quality. The nutritional way has been considered to be a useful method for improving fish flesh quality. This study using the same growth experiment as our previous study was conducted to investigate whether vitamin A could improve flesh quality by enhancing antioxidative ability via Nrf2/Keap1 signaling in fish muscle. Six diets with different levels of vitamin A were fed to grass carp (Ctenopharyngodon idella) (262.02 ± 0.45 g) for 10 weeks. Dietary vitamin A significantly improved flesh sensory appeal and nutritional value, as evident by higher pH24h value, water-holding capacity, shear force, contents of protein, lipid, four indispensable amino acids (lysine, methionine, threonine, and arginine) and total polyunsaturated fatty acid in the muscle. Furthermore, dietary vitamin A reduced oxidative damage, as evident by decreased levels of muscle reactive oxygen species, malondialdehyde, and protein carbonyl, enhanced activities of antioxidative enzyme (catalase, copper/zinc superoxide dismutase (CuZnSOD), MnSOD, glutathione peroxidase, and glutathione reductase), as well as increased content of glutathione, which was probably in relation to the activation of nuclear factor erythroid 2-related factor 2 (Nrf2) signaling. These findings demonstrated that dietary vitamin A improved flesh quality probably by enhancing antioxidant ability through Nrf2/Keap 1a signaling in fish.

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“…Vitamin A deficiency impairs retinol metabolism and reduces growth and impairs intestinal immune function in fish (Zhang et al, 2017). Vitamin A deficiency also impairs the physical barrier function associated with impaired antioxidant capacity and exacerbates apoptosis (Jiang et al, 2019). However, berberine hydrochloride can affect retinol metabolism and can up-regulate the RDH12 gene in flavanol metabolism (AH < CK < BHAH < BH).…”

Section: Discussionmentioning

confidence: 99%

Effect of Berberine Hydrochloride Treatment on Transcriptomic Response in Gill Tissue of Charybdis japonica Infected With Aeromonas hydrophila

et al. 2021

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Berberine hydrochloride is the main active ingredient of Coptis chinensis, which has demonstrated significant antibacterial activity against bacteria. However, the molecular mechanism underlying its effects is unclear in crabs. In this study, we used transcriptome sequencing to investigate the molecules involved in the therapeutic and defensive responses of Charybdis japonica infected with 105 colony-forming units (CFU)/L of Aeromonas hydrophila following treatment with berberine hydrochloride. A total of 5,409 differentially expressed genes were observed after exposure of C. japonicus for 72 h to 100 mg/L berberine hydrochloride, 100 mg/L berberine hydrochloride plus injection of 105 CFU/L A. hydrophila, and a control group injected with equal amount of physiological saline solution. Enrichment analysis revealed that these genes were involved in metabolism, cellular processes, signal transduction, and immune function. The transcriptomic results indicated that exposure to berberine hydrochloride activated glutathione metabolism, oxidative phosphorylation, fatty acid degradation, retinol metabolism, pyruvate metabolism, and the hypoxia-inducible factor 1 signaling pathway. Transcriptomic analysis and relative mRNA level analysis also identified acid hydrolase genes and phagosomal genes as key factors regulating the adaptation of acid berberine-infused C. japonicus to infection with A. hydrophila, Based on the ability of berberine hydrochloride to induce acid hydrolase and phagosomal gene expression, which can in turn remove bacteria, immersion in berberine hydrochloride may remove A. hydrophila and thus improve the survival of C. japonica. The results of this study provided a new scientific basis for the potential role of berberine hydrochloride in the immune mechanisms of crabs.

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Vitamin A deficiency impairs intestinal physical barrier function of fish

Cited by 40 publications

References 69 publications

Disruption of gut integrity and permeability contributes to enteritis in a fish-parasite model: a story told from serum metabolomics

Disruption of gut integrity and permeability contributes to enteritis in a fish-parasite model: a story told from serum metabolomics

Dietary Vitamin A Improved the Flesh Quality of Grass Carp (Ctenopharyngodon idella) in Relation to the Enhanced Antioxidant Capacity through Nrf2/Keap 1a Signaling Pathway

Effect of Berberine Hydrochloride Treatment on Transcriptomic Response in Gill Tissue of Charybdis japonica Infected With Aeromonas hydrophila

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