Vitality of Oligozoospermic Semen Samples Is Improved by Both Swim-Up and Density Gradient Centrifugation Before Cryopreservation

Counsel, Madeleine; Bellinge, R H S; Burton, Paul

doi:10.1023/b:jarg.0000031245.39921.2c

Cited by 16 publications

(8 citation statements)

References 20 publications

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“…It is common to find a reduction of up to 50% in viable cells after cryopreservation, even in fertile men (Vieira et al, 2012). Our post-thaw viability recovery rate (76%) is in agreement with values expected and reported in the literature (Counsel, Bellinge, & Burton, 2004;Ordoqui et al, 2018;Verheyen et al, 1993;World Health Organization, 2010). Upon analysing the total number of samples banked over the 28-year period, the results showed that the median of the sperm values were normal according to the WHO 2010…”

Section: Discussionsupporting

confidence: 91%

The Uruguayan semen donor population: A twenty‐eight‐year retrospective study

Barrera¹,

Ordoqui²,

Montes³

et al. 2019

Andrologia

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Several studies have reported a global decline in seminal quality over the years. The objective of this study was to describe the semen donor population of Uruguay through comparing data of successive samples banked by the same donors and the analysis of their semen and physical characteristics, ancestry origin and educational level. A total of 3,449 ejaculated samples collected from 71 donors, cryobanked between 1989 and March 2017 at Fertilab, were analysed. Results revealed a mean age of 23.90 ± 3.98 years, an average weight of 74.95 ± 1.09 kg and a mean height of 1.78 ± 0.06 m. The majority of the donors trace their origin to Europe (74.65%, 53/71) and 66.19% (47/71) have a level of education higher than secondary school.We observed longitudinal differences in two parameters, that is sperm concentration and semen volume. Sperm concentration declined, while semen volume increased significantly over the 28-year period. The results of the present study are in accordance with that of previous articles that also reported a decline in sperm concentration over time. However, no differences were observed in total sperm number per ejaculate due to the increase in semen volume values, thus reflecting no real changes in sperm production over time. K E Y W O R D Sbanking, cryopreservation, donor, spermatozoa

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Section: Discussionsupporting

confidence: 91%

The Uruguayan semen donor population: A twenty‐eight‐year retrospective study

Barrera¹,

Ordoqui²,

Montes³

et al. 2019

Andrologia

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“…Interestingly, authors have more recently argued that membrane fluidity has instead a biphasic relationship with cryogenic success and thus becomes optimal when it is balanced between preventing the cryopreservation-induced disruption of sperm membrane architecture and allowing the free entry or exit of CPAs for maximal cryoprotection (Martinez-Soto et al, 2013;Miller et al, 2005). Interestingly, a number of studies have reported favourable post-thaw results when following the prefreeze approach (Perez-Sanchez et al, 1999;Counsel et al, 2004;Petyin et al, 2004;Esteves et al, 2000;Brugnon et al, 2013), whereas additional conflicting studies have either showed comparable outcomes or the opposite to be true (Grizard et al, 1999;Donnely et al, 2001;Sharitha and Bongso, 2001;Palomar Rios et al, 2018). Comparative evaluation of these studies remains however challenging owing to the methodological disparities being present between protocols used, with a number of these studies notably neglecting to complete the cryopreservation process by not including CPA removal as the final step.…”

Section: Discussionmentioning

confidence: 99%

Is pre-freeze sperm preparation more advantageous than post-freeze?

Androni

Dodds

Tomlinson

et al. 2021

Reproduction and Fertility

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Human sperm cryopreservation is characterised to this day by sub-optimal success rates. Interestingly, a traditional approach to improving post-thaw outcome has been to integrate standard sperm preparation techniques into freezing protocols as a means of selecting sperm with the highest fertilisation potential prior to insemination. However, no consensus has been reached yet regarding the optimal timing (before or after freezing) of this selection step. Following analysis of a total of 20 human semen samples, which were divided into two aliquots prepared by density gradient centrifugation either before or after freezing, this study demonstrated higher post-thaw total (p<0.0001), progressively motile (p=0.005) and vital (p<0.0001) sperm counts for frozen prepared semen samples. The present study suggests that direct insemination with frozen-prepared sperm with minimal intervening post-thaw processing might be a more advantageous approach to current clinical practices, particularly for donor and patient intrauterine insemination programmes. Further research into the cryopreservation-induced coiled sperm tail morphology is also warranted.

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“… 22 23 24 25 Density gradient purification or centrifugation has also been shown to improve cryosurvival specifically in oligozoospermic, nononcologic controls. 26 27 There is only a single study evaluating artificial motility enhancers, PX and DOA, in testicular cancer patients. 11 There are no previous studies evaluating the use of various freezing protocols or density gradient purification on cryosurvival in testicular cancer.…”

Section: Discussionmentioning

confidence: 99%

Predictors of sperm recovery after cryopreservation in testicular cancer

Hotaling¹,

Patel²,

Vendryes³

et al. 2016

Asian J Androl

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Our objective was to identify predictors of improved postthaw semen quality in men with testicular cancer banking sperm for fertility preservation. We reviewed 173 individual semen samples provided by 67 men with testicular germ cell tumor (TGCT) who cryopreserved sperm before gonadotoxic treatment between 1994 and 2010 at our tertiary university medical center. Our main outcomes measures were independent predictors for the greater postthaw total motile count (TMC) in men with TGCT. Men with NSGCT were more likely to be younger (P < 0.01) and had high cancer stage (II or III, P < 0.01) compared with men with seminoma. In our multiple regression model, NSGCT histology, use of density gradient purification, and fresh TMC > median fresh TMC each had increased odds of a postthaw TMC greater than median postthaw TMC. Interestingly, age, advanced cancer stage (II or III), rapid freezing protocol, and motility enhancer did not show increased odds of improved postthaw TMC in our models. In conclusion, men with TGCT or poor fresh TMC should consider preserving additional vials (at least 15 vials) before oncologic treatment. Density gradient purification should be routinely used to optimize postthaw TMC in men with TGCT. Larger, randomized studies evaluating cancer stage and various cryopreservation techniques are needed to assist in counseling men with TGCT regarding fertility preservation and optimizing cryosurvival.

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Vitality of Oligozoospermic Semen Samples Is Improved by Both Swim-Up and Density Gradient Centrifugation Before Cryopreservation

Cited by 16 publications

References 20 publications

The Uruguayan semen donor population: A twenty‐eight‐year retrospective study

The Uruguayan semen donor population: A twenty‐eight‐year retrospective study

Is pre-freeze sperm preparation more advantageous than post-freeze?

Predictors of sperm recovery after cryopreservation in testicular cancer

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