Vitale Reaktionen

Lindner, J.; Schleyer, F

doi:10.1007/bf00576827

Cited by 9 publications

(8 citation statements)

References 2 publications

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“…These include reports on biochemical changes [1,6,7,9,11,13,15,16,25] see 20]. The previously suggested "signs of vitality" such as bleeding and the development of fibrin clots in the wound area [4,5,27] have also been questioned [14,26,28] and the detection of hemostatic plugs in skin wounds after short survival times is also of very limited use [12]. Furthermore, it has been reported that information on wound vitality can be obtained by changes in the staining pattern of the basic connective tissue substances [17], but on the basis of routine histology this approach provided no valuable parameters for estimation of wound vitality.…”

Section: Introductionmentioning

confidence: 99%

The immunohistochemical localization of alpha1-antichymotrypsin and fibronectin and its meaning for the determination of the vitality of human skin wounds

et al. 1993

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Summary.A total of 39 vital human skin wounds (20 cases with short survival times ranging from a few seconds to approximately 30 min and 19 cases with wound ages between 50rain and 13 days) were investigated. Alphalantichymotrypsin (A1-ACT) was visualized by immunohistochemistry. Additionally, fibronectin was localized in 13 of these wounds (8 cases with short and 5 cases with longer survival times). Furthermore, 13 postmortem lesions (stab wounds) were removed from corpses approximately 4 h after infliction and analyzed for A1-ACT and fibronectin. The "vital" reaction previously described for A1-ACT in form of a band-shaped staining pattern at the wound edges was observed in both vital wounds and in most postmortem lesions. A similar reaction was also obtained for fibronectin in wounds inflicted after death, but could be unambiguously distinguished from vital fibronectin staining by morphological criteria. Therefore, it seems questionable that the vitality of skin wounds can be determined by the immunohistochemical detection of A~-ACT and probably other proteinase inhibitors. The meaning of the localization of fibronectin for the determination of the vitality of human skin wounds with a survival time of at least a few minutes could be confirmed.

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Section: Introductionmentioning

confidence: 99%

The immunohistochemical localization of alpha1-antichymotrypsin and fibronectin and its meaning for the determination of the vitality of human skin wounds

et al. 1993

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“…The enduring proliferative activity of the skin, therefore, represents an exception, which has been substantiated by morphological and biochemical findings. The relationship between oxygen consumption of the skin and postmortal interval was investigated by three research teams [1,5,15]: All observed a decrease of oxygen consumption with increasing postmortal interval. The relationship between oxygen consumption of the skin and postmortal interval was investigated by three research teams [1,5,15]: All observed a decrease of oxygen consumption with increasing postmortal interval.…”

Section: Discussionmentioning

confidence: 99%

“…The relationship between oxygen consumption of the skin and postmortal interval was investigated by three research teams [1,5,15]: All observed a decrease of oxygen consumption with increasing postmortal interval. Lindner [15] and Berg et ah [1] investigated the incorporation of 35S sulfate in subepidermal connective tissue and observed incorporation up to 3 days post mortem in tissue stored at low temperatures. Lindner [15] and Berg et ah [1] investigated the incorporation of 35S sulfate in subepidermal connective tissue and observed incorporation up to 3 days post mortem in tissue stored at low temperatures.…”

Section: Discussionmentioning

confidence: 99%

Cytokinetics of epidermic cells in skin from human cadavers

1988

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Punch biopsies taken from the extensor side of the thigh of 35 human cadavers were incubated in vitro with radioactively labeled thymidine (3H-thymidine = 3H-TdR, 14C-thymidine = ~4C-TdR) to determine whether a relationship exists between changes in the proliferative activity of the skin and the postmortal interval after irreversible cardiac arrest. The cadavers were stored at 4°C. Cadavers with indeterminable time of death or presence of intoxication, drug therapy with cytostaic agents, or a skin disease were excluded from the study. Single, double, or multiple biopsies were performed on the same cadaver; single labeling with 3H-TdR was done in all cases; double labeling with 3H-TdR and a4C-TdR in selected cases.No relevant changes in the labeling index (mean, 2.39 _+ 1.03%) were demonstrable within the examined postmortal interval of 77.75 h. The DNA synthesis time (t~) was, on the average, 4.75 + 1.44h; a certain relationship to the postmortal interval existed since ts declined with increasing storage time. The potential doubling time (tpot) decreased accordingly, beginning with 181.7 h (mean storage time, 29.9 h) and ending with 137.7 h (mean storage time, 41.7 h). No statistically relevant differences, however, were established at the 1% level. Whereas both labeling index and tpot during the early postmortal interval are comparable with observations in live humans, ts was relatively short as compared to that for the epidermis of live humans.

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“…Die histomorphologische Analyse des Wundgebietes ist eine seit langem durchgesetzte Informationsquelle. In ihren Untersuchungen berichten manche Autoren (Raekallio 1961(Raekallio , 1965Lindner 1962Lindner , 1967Friebel und Woohsmann 1968;Berg 1972;Kontzevich et al 1980), dab schon in den frtihen Zeitabschnitten nach Verletzung die Merkmale der vaskular-zellul~iren Reaktion w~ihrend der Entziindungsentwicklung im Wundgebiet erscheinen. Zusammen mit den Ver~inderungen in der zellul~iren Zusammensetzung der gesch~idigten Gewebe macht sich dort die Bildung zweier Zonen -einer zentralen und einer peripheren -bemerkbar.…”

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Informative M�glichkeiten der Wundoberfl�che f�r Vitalit�tsbeweis und Wundaltersbestimmung bei Hautschnittwunden

Lasarov

1989

Z Rechtsmed

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A histological study was carried out on skin cut wounds of guinea pigs at various time intervals during the first day after infliction of the cut. At the same time intervals, blood cells were obtained from the wound surface prints and analyzed cytologically and cytochemically. Histological examination showed that a central and peripheral zone formed in the wound area, demonstrating that necrobiotic and inflammatory processes were even occurring in the early hours after injury. However, changes in the quantitative relationships between individual kinds of cells were much more differentiated cytologically and reflected much more precisely. Functional and structural macrophage and lymphocyte changes in the wound region during inflammation were also revealed. In correlation, cytochemical analysis confirmed the concept concerning the development of the inflammatory process in the wound area, as enzyme-activity changes clearly detectable, which reflected the rapid, energetic, plastic cellular processes on the wound surface. Our combined results suggest that the wound surface is a zone of vital processes, but that it is not a "dead," "necrobiotic" area. The dynamics of the cellular alterations in the wound surface, reflecting the vital processes developing there, can be successfully used when the problem of vitality, especially the time lapse after the skin injury, is to be resolved.

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Vitale Reaktionen

Cited by 9 publications

References 2 publications

The immunohistochemical localization of alpha1-antichymotrypsin and fibronectin and its meaning for the determination of the vitality of human skin wounds

The immunohistochemical localization of alpha1-antichymotrypsin and fibronectin and its meaning for the determination of the vitality of human skin wounds

Cytokinetics of epidermic cells in skin from human cadavers

Informative M�glichkeiten der Wundoberfl�che f�r Vitalit�tsbeweis und Wundaltersbestimmung bei Hautschnittwunden

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