Vital for Viruses: Intrinsically Disordered Proteins

Dyson, H. Jane

doi:10.1016/j.jmb.2022.167860

Cited by 5 publications

(3 citation statements)

References 120 publications

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“…However, no significant differences were observed between the average IUPred3 and ANCHOR2 values among the viral subpopulations with distinct IFN-inducing phenotypes ( Supplementary Figure S2 ). Given the ubiquitous nature of ID vRNAs, the proposed internally disordered polypeptides may play a critical role in cellular processes, cell signaling, and immunomodulation during viral infection [ 64 , 65 , 66 ].…”

Section: Resultsmentioning

confidence: 99%

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Diversity and Complexity of Internally Deleted Viral Genomes in Influenza A Virus Subpopulations with Enhanced Interferon-Inducing Phenotypes

Ghorbani,

Ngunjiri,

Rendon

et al. 2023

Viruses

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Influenza A virus (IAV) populations harbor large subpopulations of defective-interfering particles characterized by internally deleted viral genomes. These internally deleted genomes have demonstrated the ability to suppress infectivity and boost innate immunity, rendering them promising for therapeutic and immunogenic applications. In this study, we aimed to investigate the diversity and complexity of the internally deleted IAV genomes within a panel of plaque-purified avian influenza viruses selected for their enhanced interferon-inducing phenotypes. Our findings unveiled that the abundance and diversity of internally deleted viral genomes were contingent upon the viral subculture and plaque purification processes. We observed a heightened occurrence of internally deleted genomes with distinct junctions in viral clones exhibiting enhanced interferon-inducing phenotypes, accompanied by additional truncation in the nonstructural 1 protein linker region (NS1Δ76-86). Computational analyses suggest the internally deleted IAV genomes can encode a broad range of carboxy-terminally truncated and intrinsically disordered proteins with variable lengths and amino acid composition. Further research is imperative to unravel the underlying mechanisms driving the increased diversity of internal deletions within the genomes of viral clones exhibiting enhanced interferon-inducing capacities and to explore their potential for modulating cellular processes and immunity.

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Section: Resultsmentioning

confidence: 99%

“…They are highly versatile and can interact with various binding partners, including other proteins, nucleic acids, and small molecules. This versatility allows them to participate in numerous cellular processes and pathways, including molecular recognition, cell signaling and regulation, and immunomodulation [ 64 , 80 , 81 ].…”

Section: Discussionmentioning

confidence: 99%

Diversity and Complexity of Internally Deleted Viral Genomes in Influenza A Virus Subpopulations with Enhanced Interferon-Inducing Phenotypes

Ghorbani,

Ngunjiri,

Rendon

et al. 2023

Viruses

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“…As nearly all viruses encode intrinsically disordered proteins that are inherently prone to undergo phase separation (Dyson, 2023), it is conceivable that condensate formation by plant viruses is commonplace. As described above, several plant viruses form condensates that are crucial for RNA replication and virus movement.…”

Section: Figmentioning

confidence: 99%

Plant viruses and biomolecular condensates: novel perspectives in virus replication strategies

May

2024

New Phytologist

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Biomolecular condensates are dynamic, membraneless, cellular compartments formed by the reversible assembly of proteins, nucleic acids, and other biomolecules (Hyman et al., 2014). The functions of these condensates in cellular processes have been extensively studied in mammalian systems (Yoshizawa et al., 2020), but similar studies in plants have traditionally lagged far behind. However, in the past c. 5 years, a flurry of research in plants has revealed that biomolecular condensates play crucial roles in cellular organization and functions. These functions are wide-ranging and include flowering, stress responses, RNA processing, and DNA damage, among others (Liu et al., 2024). Like cellular proteins, viral proteins can form condensates during infection, contributing significantly to virus replication cycles. While the functions of biomolecular condensates formed by viral pathogens in mammals are well-documented (Zhang et al., 2023), our understanding of the functions of condensates formed by plant viruses is limited. In an article published in this issue of New Phytologist, Lin & Nagy (2024; 1917-1935 demonstrate that biomolecular condensates formed during Tomato bushy stunt virus (TBSV, Tombusviridae family) infection sequester glycolytic enzymes and increase local adenosine triphosphate (ATP) concentrations for virus replication. These findings advance our understanding of the pro-viral roles of biomolecular condensates, broadening possibilities for how plant viruses concentrate biomolecules beyond traditional membrane-bound virus inclusions formed during infection.

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Multivalency emerges as a common feature of intrinsically disordered protein interactions

Sipko,

Chappell,

Berlow

2024

Current Opinion in Structural Biology

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Vital for Viruses: Intrinsically Disordered Proteins

Cited by 5 publications

References 120 publications

Diversity and Complexity of Internally Deleted Viral Genomes in Influenza A Virus Subpopulations with Enhanced Interferon-Inducing Phenotypes

Diversity and Complexity of Internally Deleted Viral Genomes in Influenza A Virus Subpopulations with Enhanced Interferon-Inducing Phenotypes

Plant viruses and biomolecular condensates: novel perspectives in virus replication strategies

Multivalency emerges as a common feature of intrinsically disordered protein interactions

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