Visually predicting microRNA-regulated tumor metastasis by intracellularly 3D counting of fluorescent spots based on in situ growth of DNA flares

Spherical Nucleic Acid-Mediated Spatial Matching-Guided Nonenzymatic DNA Circuits for the Prediction and Prevention of Malignant Tumor Invasion

“…Due to the low sensitivity, the fluorescent probes of one-to-one cannot accurately sense the fluctuation in miRNA expression level at a low concentration, which is not conducive to “find” the indicator of early malignant transformation. Recently, researchers have designed sensitive probes for the detection of miRNA based on different signal amplification strategies, such as strand displacement reaction (SDR). , However, common fluorescent DNA probes are easily degraded by active nucleases existing in biological mediums, inevitably generating false positive signals and limiting the applications in miRNA imaging in living cells and in vivo.…”

“…Recently, researchers have designed sensitive probes for the detection of miRNA based on different signal amplification strategies, such as strand displacement reaction (SDR). 18,19 However, common fluorescent DNA probes are easily degraded by active nucleases existing in biological mediums, inevitably generating false positive signals and limiting the applications in miRNA imaging in living cells and in vivo.…”