Visualizing multiple constrictions in spheroidal Escherichia coli cells

Zaritsky, Arieh; Geel, Anton van; Fishov, Itzhak; Pas, E; Einav, Monica; Woldringh, Conrad L.

doi:10.1016/s0300-9084(99)00206-0

Cited by 15 publications

(5 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In C. paradoxa and the rodA mutant KLB24, septation starts from a single point of the sphere, with localized invagination growing further outward and eventually encompassing the whole cell 26,28 . FtsZ shows a similar pattern: it starts to localize as a single point, which grows into arcs and eventually forms a complete ring [26][27][28] . In contrast to these findings, in Rhs, FtsZ does not nucleate from a single point only (Fig.…”

Section: Asynchronous Division By Non-ring Ftsz In the Gammaproteobacterial Symbiont Of Robbea Hypermnestramentioning

confidence: 87%

“…2k-m). Non-ring FtsZ-mediated invagination has been observed in the cyanelles of the glaucophyte Cyanophora paradoxa 26 and spherical E. coli 27 . In C. paradoxa and the rodA mutant KLB24, septation starts from a single point of the sphere, with localized invagination growing further outward and eventually encompassing the whole cell 26,28 .…”

Section: Asynchronous Division By Non-ring Ftsz In the Gammaproteobacterial Symbiont Of Robbea Hypermnestramentioning

confidence: 99%

See 1 more Smart Citation

Asynchronous division by non-ring FtsZ in the gammaproteobacterial symbiont of Robbea hypermnestra

et al. 2016

View full text Add to dashboard Cite

The reproduction mode of uncultivable microorganisms deserves investigation as it can largely diverge from conventional transverse binary fission. Here, we show that the rod-shaped gammaproteobacterium thriving on the surface of the Robbea hypermnestra nematode divides by FtsZ-based, non-synchronous invagination of its poles-that is, the host-attached and fimbriae-rich pole invaginates earlier than the distal one. We conclude that, in a naturally occurring animal symbiont, binary fission is host-oriented and does not require native FtsZ to polymerize into a ring at any septation stage.

show abstract

Section: Asynchronous Division By Non-ring Ftsz In the Gammaproteobacterial Symbiont Of Robbea Hypermnestramentioning

confidence: 87%

Section: Asynchronous Division By Non-ring Ftsz In the Gammaproteobacterial Symbiont Of Robbea Hypermnestramentioning

confidence: 99%

Asynchronous division by non-ring FtsZ in the gammaproteobacterial symbiont of Robbea hypermnestra

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Such phenomenon is supported by the observation that the T -period is more or less constant for doubling times shorter than 60 min ( Woldringh et al, 1977 ; Zaritsky et al, 2006 ). Under conditions of thymine limitation, when cell width increases without a compensating change in growth rate ( Zaritsky and Pritchard, 1973 ), the D -period has been found to increase ( Zaritsky et al, 1999 ).…”

Section: Discussionmentioning

confidence: 99%

Different Amounts of DNA in Newborn Cells of Escherichia coli Preclude a Role for the Chromosome in Size Control According to the “Adder” Model

2018

Self Cite

View full text Add to dashboard Cite

According to the recently-revived adder model for cell size control, newborn cells of Escherichia coli will grow and divide after having added a constant size or length, ΔL, irrespective of their size at birth. Assuming exponential elongation, this implies that large newborns will divide earlier than small ones. The molecular basis for the constant size increment is still unknown. As DNA replication and cell growth are coordinated, the constant ΔL could be based on duplication of an equal amount of DNA, ΔG, present in newborn cells. To test this idea, we measured amounts of DNA and lengths of nucleoids in DAPI-stained cells growing in batch culture at slow and fast rates. Deeply-constricted cells were divided in two subpopulations of longer and shorter lengths than average; these were considered to represent large and small prospective daughter cells, respectively. While at slow growth, large and small prospective daughter cells contained similar amounts of DNA, fast growing cells with multiforked replicating chromosomes, showed a significantly higher amount of DNA (20%) in the larger cells. This observation precludes the hypothesis that ΔL is based on the synthesis of a constant ΔG. Growth curves were constructed for siblings generated by asymmetric division and growing according to the adder model. Under the assumption that all cells at the same growth rate exhibit the same time between initiation of DNA replication and cell division (i.e., constant C+D-period), the constructions predict that initiation occurs at different sizes (Li) and that, at fast growth, large newborn cells transiently contain more DNA than small newborns, in accordance with the observations. Because the state of segregation, measured as the distance between separated nucleoids, was found to be more advanced in larger deeply-constricted cells, we propose that in larger newborns nucleoid separation occurs faster and at a shorter length, allowing them to divide earlier. We propose a composite model in which both differential initiation and segregation leads to an adder-like behavior of large and small newborn cells.

show abstract

“…If we leave the FtsZ density constant in the enlarged cell, then FtsZ rings still can form. Experimental observations in spherical mutant E. coli cells first exhibit FtsZ arcs instead of rings [20,21], which mature later into rings, presumably because of ongoing FtsZ production in the cell that increases the FtsZ density [20]. This suggests that the initial FtsZ concentration is lower in these cells compared to wild type cells.…”

Section: Discussionmentioning

confidence: 99%

Modeling FtsZ ring formation in the bacterial cell—anisotropic aggregation via mutual interactions of polymer rods

Fischer‐Friedrich

Gov

2011

Phys. Biol.

View full text Add to dashboard Cite

The cytoskeletal protein FtsZ polymerizes to a ring structure (Z ring) at the inner cytoplasmic membrane that marks the future division site and scaffolds the division machinery in many bacterial species. FtsZ is known to polymerize in the presence of GTP into single-stranded protofilaments. In vivo, FtsZ polymers become associated with the cytoplasmic membrane via interaction with the membrane-binding proteins FtsA and ZipA. The FtsZ ring structure is highly dynamic and undergoes constantly polymerization and depolymerization processes and exchange with the cytoplasmic pool. In this theoretical study, we consider a scenario of Z ring self-organization via self-enhanced attachment of FtsZ polymers due to end-to-end interactions and lateral interactions of FtsZ polymers on the membrane. With the assumption of exclusively circumferential polymer orientations, we derive coarse-grained equations for the dynamics of the pool of cytoplasmic and membrane-bound FtsZ. To capture stochastic effects expected in the system due to low particle numbers, we simulate our computational model using a Gillespie-type algorithm. We obtain ring- and arc-shaped aggregations of FtsZ polymers on the membrane as a function of monomer numbers in the cell. In particular, our model predicts the number of FtsZ rings forming in the cell as a function of cell geometry and FtsZ concentration. We also calculate the time of FtsZ ring localization to the midplane in the presence of Min oscillations. Finally, we demonstrate that the assumptions and results of our model are confirmed by 3D reconstructions of fluorescently-labeled FtsZ structures in E. coli that we obtained.

show abstract

Visualizing multiple constrictions in spheroidal Escherichia coli cells

Cited by 15 publications

References 38 publications

Asynchronous division by non-ring FtsZ in the gammaproteobacterial symbiont of Robbea hypermnestra

Asynchronous division by non-ring FtsZ in the gammaproteobacterial symbiont of Robbea hypermnestra

Different Amounts of DNA in Newborn Cells of Escherichia coli Preclude a Role for the Chromosome in Size Control According to the “Adder” Model

Modeling FtsZ ring formation in the bacterial cell—anisotropic aggregation via mutual interactions of polymer rods

Contact Info

Product

Resources

About