Visualizing metal ions in cells: An overview of analytical techniques, approaches, and probes

Dean, Kevin M.; Qin, Yan; Palmer, Amy E.

doi:10.1016/j.bbamcr.2012.04.001

Cited by 133 publications

(121 citation statements)

References 132 publications

Supporting

Mentioning

113

Contrasting

Unclassified

Order By: Relevance

“…Intensity-based probes are 'turn-on' fluorophores, and display a chelation-dependent increase in fluorescence intensity of up to 100-fold. Most of these probes are based on the modulation in the photon-induced electron transfer phenomenon (16)(17)(18) . Briefly, the probes are composed of a fluorophore, a spacer domain and an electron-rich metal chelate.…”

Section: Imaging Free Zn 2+ In Living Cellsmentioning

confidence: 99%

See 1 more Smart Citation

Intracellular zinc in insulin secretion and action: a determinant of diabetes risk?

et al. 2015

View full text Add to dashboard Cite

Zinc is an important micronutrient, essential in the diet to avoid a variety of conditions associated with malnutrition such as diarrhoea and alopecia. Lowered circulating levels of zinc are also found in diabetes mellitus, a condition which affects one in twelve of the adult population and whose treatments consume approximately 10 % of healthcare budgets. Zn 2+ ions are essential for a huge range of cellular functions and, in the specialised pancreatic β-cell, for the storage of insulin within the secretory granule. Correspondingly, genetic variants in the SLC30A8 gene, which encodes the diabetes-associated granule-resident Zn 2+ transporter ZnT8, are associated with an altered risk of type 2 diabetes. Here, we focus on (i) recent advances in measuring free zinc concentrations dynamically in subcellular compartments, and (ii) studies dissecting the role of intracellular zinc in the control of glucose homeostasis in vitro and in vivo. We discuss the effects on insulin secretion and action of deleting or overexpressing Slc30a8 highly selectively in the pancreatic β-cell, and the role of zinc in insulin signalling. While modulated by genetic variability, healthy levels of dietary zinc, and hence normal cellular zinc homeostasis, are likely to play an important role in the proper release and action of insulin to maintain glucose homeostasis and lower diabetes risk.

show abstract

Section: Imaging Free Zn 2+ In Living Cellsmentioning

confidence: 99%

“…Fluorescence microscopy is usually adopted and uses one of two probe types (16) . First, low molecular weight compounds which display a massive change in fluorescence intensity upon zinc chelation can be loaded into the cell.…”

Section: Imaging Free Zn 2+ In Living Cellsmentioning

confidence: 99%

Intracellular zinc in insulin secretion and action: a determinant of diabetes risk?

et al. 2015

View full text Add to dashboard Cite

show abstract

“…More than 1,000 different small organic molecule-based metal fluorescent probes have been described since 1997 that allow for quantitative analysis of dissolved metal concentrations and microscopic metal localization in biological samples in combination with CLSM (32)(33)(34)(35). However, this has been applied almost exclusively in the field of cell biology.…”

mentioning

confidence: 99%

“…However, this has been applied almost exclusively in the field of cell biology. In this review, we focus on specific metal-induced rhodamine spirolactam ring-opening "turn-on"-type fluorescent probes (32,(35)(36)(37); we discuss strategies of how to select a metal-specific fluorescent probe for environmental research such as sorption studies of heavy metals in environmental samples such as biofilms and CEMAs to further our understanding of metal sorption under both anoxic and oxic conditions. Since, to our current knowledge, no environmental or geomicrobiology-related applications of such highly selective metal fluorescent probes were published so far, we performed a case study to ensure the applicability of such probes to heterogeneous samples of bacteriogenic CEMAs.…”

mentioning

confidence: 99%

Mapping of Heavy Metal Ion Sorption to Cell-Extracellular Polymeric Substance-Mineral Aggregates by Using Metal-Selective Fluorescent Probes and Confocal Laser Scanning Microscopy

Hao

Kappler

et al. 2013

Appl Environ Microbiol

View full text Add to dashboard Cite

b Biofilms, organic matter, iron/aluminum oxides, and clay minerals bind toxic heavy metal ions and control their fate and bioavailability in the environment. The spatial relationship of metal ions to biomacromolecules such as extracellular polymeric substances (EPS) in biofilms with microbial cells and biogenic minerals is complex and occurs at the micro-and submicrometer scale. Here, we review the application of highly selective and sensitive metal fluorescent probes for confocal laser scanning microscopy (CLSM) that were originally developed for use in life sciences and propose their suitability as a powerful tool for mapping heavy metals in environmental biofilms and cell-EPS-mineral aggregates (CEMAs). The benefit of using metal fluorescent dyes in combination with CLSM imaging over other techniques such as electron microscopy is that environmental samples can be analyzed in their natural hydrated state, avoiding artifacts such as aggregation from drying that is necessary for analytical electron microscopy. In this minireview, we present data for a group of sensitive fluorescent probes highly specific for Fe 3؉ , Cu 2؉ , Zn 2؉ , and Hg 2؉ , illustrating the potential of their application in environmental science. We evaluate their application in combination with other fluorescent probes that label constituents of CEMAs such as DNA or polysaccharides and provide selection guidelines for potential combinations of fluorescent probes. Correlation analysis of spatially resolved heavy metal distributions with EPS and biogenic minerals in their natural, hydrated state will further our understanding of the behavior of metals in environmental systems since it allows for identifying bonding sites in complex, heterogeneous systems. Biofilms are the dominant form of microbial life on Earth (1), and the organic material that is present in biofilms significantly impacts the cycling and sequestration of toxic heavy metals in the environment (2, 3). The underlying sorption and complexation mechanisms are difficult to evaluate (4), since biofilms are highly dynamic and complex structures that consist of diverse biomacromolecules (5) and the in situ heavy metal distributions are readily influenced by common invasive analysis approaches such as sequential extractions for the determination of different metal fractions. Here, we introduce a promising approach for studying the distribution and sorption of heavy metals in biofilms and cell-extracellular polymeric substance (EPS)-mineral aggregates (CEMAs) under close-to-natural conditions using confocal laser scanning microscopy (CLSM) in combination with highly selective metal ion-sensitive fluorescence probes. This approach is frequently used for metal detection in cell biology but was rarely applied in environmental and geomicrobiology research due to a former lack of highly selective fluorescence probes and due to the complexity of environmental biofilms and CEMAs.Biofilms are mainly composed of water, microorganisms, and an EPS matrix (6) that consists of mostly polysacchari...

show abstract

“…Zinc-responsive fluorescent reporters are well suited for this purpose. Of the various classes of zinc sensors (14), small molecule fluorescein-based scaffolds are the most broadly implemented (3,15). Fluorescein is bright (eΦ), nontoxic, and compatible with one-and twophoton microscopy (16).…”

mentioning

confidence: 99%

Reaction-based fluorescent sensor for investigating mobile Zn ²⁺ in mitochondria of healthy versus cancerous prostate cells

Chyan

Zhang

Lippard

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

148

121

View full text Add to dashboard Cite

Chelatable, mobile forms of divalent zinc, Zn(II), play essential signaling roles in mammalian biology. A complex network of zinc import and transport proteins has evolved to control zinc concentration and distribution on a subcellular level. Understanding the action of mobile zinc requires tools that can detect changes in Zn(II) concentrations at discrete cellular locales. We present here a zinc-responsive, reaction-based, targetable probe based on the diacetyled form of Zinpyr-1. The compound, (6-amidoethyl)triphenylphosphonium Zinpyr-1 diacetate (DA-ZP1-TPP), is essentially nonfluorescent in the metal-free state; however, exposure to Zn (II) triggers metal-mediated hydrolysis of the acetyl groups to afford a large, rapid, and zinc-induced fluorescence response. DA-ZP1-TPP is insensitive to intracellular esterases over a 2-h period and is impervious to proton-induced turn-on. A TPP unit is appended for targeting mitochondria, as demonstrated by live cell fluorescence imaging studies. The practical utility of DA-ZP1-TPP is demonstrated by experiments revealing that, in contrast to healthy epithelial prostate cells, tumorigenic cells are unable to accumulate mobile zinc within their mitochondria.reaction-based probe | prostate cancer | zinc biology | fluorescence microscopy

show abstract

Visualizing metal ions in cells: An overview of analytical techniques, approaches, and probes

Cited by 133 publications

References 132 publications

Intracellular zinc in insulin secretion and action: a determinant of diabetes risk?

Intracellular zinc in insulin secretion and action: a determinant of diabetes risk?

Mapping of Heavy Metal Ion Sorption to Cell-Extracellular Polymeric Substance-Mineral Aggregates by Using Metal-Selective Fluorescent Probes and Confocal Laser Scanning Microscopy

Reaction-based fluorescent sensor for investigating mobile Zn ²⁺ in mitochondria of healthy versus cancerous prostate cells

Contact Info

Product

Resources

About

Visualizing metal ions in cells: An overview of analytical techniques, approaches, and probes

Cited by 133 publications

References 132 publications

Intracellular zinc in insulin secretion and action: a determinant of diabetes risk?

Intracellular zinc in insulin secretion and action: a determinant of diabetes risk?

Mapping of Heavy Metal Ion Sorption to Cell-Extracellular Polymeric Substance-Mineral Aggregates by Using Metal-Selective Fluorescent Probes and Confocal Laser Scanning Microscopy

Reaction-based fluorescent sensor for investigating mobile Zn 2+ in mitochondria of healthy versus cancerous prostate cells

Contact Info

Product

Resources

About

Reaction-based fluorescent sensor for investigating mobile Zn ²⁺ in mitochondria of healthy versus cancerous prostate cells