Visualizing hidden heterogeneity in isogenic populations of C. elegans

Wu, Deqing; Rea, Shane L.; Yashin, Anatoli I.; Johnson, Thomas E.

doi:10.1016/j.exger.2006.01.003

Cited by 71 publications

(69 citation statements)

References 43 publications

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“…1) and in previous work (Johnston et al 2008) that a mixture of at least two aging states is likely to be found in all groups of animals assayed at a given chronological age. In late life, individual variation in response to stress within an isogenic population has been previously observed by others and described as hidden heterogeneity (D. Wu et al 2006). A model consisting of two subpopulations was consistent with the observations, where a long-lived sub-population showed a robust heat-shock response and a shorter-lived sub-population had a weaker heat-shock response.…”

Section: Discussionsupporting

confidence: 87%

Molecular characterization of the transition to mid-life in Caenorhabditis elegans

Eckley

Rahimi

Mantilla

et al. 2012

AGE

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We present an initial molecular characterization of a morphological transition between two early aging states. In previous work, an age score reflecting physiological age was developed using a machine classifier trained on images of worm populations at fixed chronological ages throughout their lifespan. The distribution of age scores identified three stable post-developmental states and transitions. The first transition occurs at day 5 post-hatching, where a significant percentage of the population exists in both state I and state II. The temperature dependence of the timing of this transition (Q 10~1 .17) is too low to be explained by a stepwise process with an enzymatic or chemical rate-limiting step, potentially implicating a more complex mechanism. Individual animals at day 5 were sorted into state I and state II groups using the machine classifier and analyzed by microarray expression profiling. Despite being isogenic, grown for the same amount of time, and indistinguishable by eye, these two morphological states were confirmed to be molecularly distinct by hierarchical clustering and principal component analysis of the microarray results. These molecular differences suggest that pharynx morphology reflects the aging state of the whole organism. Our expression profiling yielded a gene set that showed significant overlap with those from three previous age-related studies and identified several genes not previously implicated in aging. A highly represented group of genes unique to this study is involved in targeted ubiquitin-mediated proteolysis, including Skp1-related (SKR), F-box-containing, and BTB motif adaptors.

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Section: Discussionsupporting

confidence: 87%

Molecular characterization of the transition to mid-life in Caenorhabditis elegans

Eckley

Rahimi

Mantilla

et al. 2012

AGE

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“…The concentration of NO in sodium nitroprusside solutions was estimated by calibration curve plotted with the use of fresh solutions of NaNO2 (Sigma Aldrich 237,213) in concentrations of 0–100 μM. Nitrites, NO oxidation products under aerobic conditions, were measured spectrophotometrically (540 nm) using Griess reagent (Sigma-Aldrich G4410) [31]. …”

Section: Nitric Oxide Production In Lactobacillusmentioning

confidence: 99%

In vitro and in vivo defensive effect of probiotic LAB against Pseudomonas aeruginosa using Caenorhabditis elegans model

et al. 2018

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This study aimed to investigate in vitro and in vivo the probiotic characteristics of lactic acid bacteria (LAB) isolated from Korean traditional fermented foods. Caenorhabditis elegans (C. elegans) was used for analytical assays of fertility, chemotaxis, life-span, worm-killing and bacterial colonization in the intestinal lumen of the worm. All 35 strains of LAB reduced fertility and slowed development in the worms. The worm-killing assay showed that LAB significantly increased the lifespan (P < 0.05) and reduced the susceptibility to virulent PA14; however, the heat-killed LAB did not. The bacterial colonization assay revealed that LAB proliferated and protected the gut of the worm against infection by Pseudomonas aeruginosa PA14. In addition, specific LAB Pediococcus acidilactici(P. acidilactici DM-9), Pediococcus brevis (L. brevis SDL1411), and Pediococcus pentosaceus (P. pentosaceus SDL1409) strains showed acid resistance (66–91%), resistance to pepsin (64–67%) and viability in simulated intestinal fluid (67–73%) based on in vitro probiotic analyses. Taken together, these results suggest that C. elegans may be a tractable model for screening efficient probiotics.

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“…Consequently, the levels of GFP fluorescence and HSP-16.2 expression are strongly correlated in TJ375. Therefore, this strain provides an accurate assessment of the total amount of native HSP-16.2 protein (Rea et al 2005;Wu et al 2006).…”

Section: Strains and Culture Conditionsmentioning

confidence: 99%

“…EG1653 and TJ375 strains have been regularly used, respectively, to quantify the expression of UNC-49 (Abraham et al 2011;Byrd et al 2001;Garafalo et al 2015;Knobel et al 2001;Rowland et al 2006) and HSP-16.2 (Abbas and Wink 2010;Lamark and Johansen 2012;Leroy et al 2012;Rea et al 2005;Shore et al 2012;Strayer et al 2003;Wang et al 2012;Wu et al 2006) based on the quantitation of their GFP fluorescence intensities.…”

Section: Strains and Culture Conditionsmentioning

confidence: 99%

“…Genetic manipulations to overexpress individual chaperones, like Hsp90 and Hsp70, resulted in a subtle extension of life span (Morley and Morimoto 2004); however, the small chaperone Hsp16 seemed to be more efficient in worms (Soti and Csermely 2007), particularly HSP-16.2 that is often used as an indicator of increases in thermal and oxidative stress (Hartwig et al 2009;Rea et al 2005;Strayer et al 2003;Wiegant et al 2009;Wu et al 2006;Xiao et al 2009). …”

Section: Introductionmentioning

confidence: 99%

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Inactivation of GABAA receptor is related to heat shock stress response in organism model Caenorhabditis elegans

Camargo

Elizalde

Trujillo

et al. 2016

Cell Stress and Chaperones

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The mechanisms underlying oxidative stress (OS) resistance are not completely clear. Caenorhabditis elegans (C. elegans) is a good organism model to study OS because it displays stress responses similar to those in mammals. Among these mechanisms, the insulin/IGF-1 signaling (IIS) pathway is thought to affect GABAergic neurotransmission. The aim of this study was to determine the influence of heat shock stress (HS) on GABAergic activity in C. elegans. For this purpose, we tested the effect of exposure to picrotoxin (PTX), gammaaminobutyric acid (GABA), hydrogen peroxide, and HS on the occurrence of a shrinking response (SR) after nose touch stimulus in N2 (WT) worms. Moreover, the effect of HS on the expression of UNC-49 (GABA A receptor ortholog) in the EG1653 strain and the effect of GABA and PTX exposure on HSP-16.2 expression in the TJ375 strain were analyzed. PTX 1 mM-or H 2 O 2 0.7 mM-exposed worms displayed a SR in about 80 % of trials. GABA exposure did not cause a SR. HS prompted the occurrence of a SR as did PTX 1 mM or H 2 O 2 0.7 mM exposure. In addition, HS increased UNC-49 expression, and PTX augmented HSP-16.2 expression. Thus, the results of the present study suggest that oxidative stress, through either H 2 O 2 exposure or application of heat shock, inactivates the GABAergic system, which subsequently would affect the oxidative stress response, perhaps by enhancing the activity of transcription factors DAF-16 and HSF-1, both regulated by the IIS pathway and related to hsp-16.2 expression.

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Visualizing hidden heterogeneity in isogenic populations of C. elegans

Cited by 71 publications

References 43 publications

Molecular characterization of the transition to mid-life in Caenorhabditis elegans

Molecular characterization of the transition to mid-life in Caenorhabditis elegans

In vitro and in vivo defensive effect of probiotic LAB against Pseudomonas aeruginosa using Caenorhabditis elegans model

Inactivation of GABAA receptor is related to heat shock stress response in organism model Caenorhabditis elegans

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