Visualizing drug transport across stratum corneum: cryotechniques, vapour fixation, autoradiography

Haan, F. H. N. de; Boddé, Harry E.; Bruijn, W. C. de; Ginsel, L.A.; Junginger, Hans E.

doi:10.1016/0378-5173(89)90063-x

Cited by 14 publications

(8 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A specially designed desiccator [7,8] was evacuated (about 1 Pa), after which the lid from the sample holder was lifted with a manipulator. Osmium tetroxide (OsO 4 ) vapor was admitted to the working chamber by opening a side port containing 0.1 g solid OsO 4 .…”

Section: Sample Preparationmentioning

confidence: 99%

Validation of Freeze-Drying to Visualize Percutaneous <sup>3</sup>H-Estradiol Transport: The Influence of Skin Hydration on the Efficacy of the Method

Neelissen¹,

Arth

Schrijvers

et al. 1998

Skin Pharmacol Physiol

Self Cite

View full text Add to dashboard Cite

A study was made of the validity of freeze-drying to visualize the distribution of ³H-estradiol in human stratum corneum after topical application of a dry dose, a patch or a buffer solution. Each of these donor formulations was applied to human dermatomed skin for 24 h using Franz permeation cells. Subsequently, small pieces of skin were subjected to cryofixation, freeze-drying, osmium tetroxide vapor fixation, Spurr resin embedding and electron microscopic autoradiography. Stratum corneum from dry dose and patch application experiments was well preserved by freeze-drying, allowing an accurate localization of ³H-estradiol. In contrast, stratum corneum from buffer solution experiments suffered from cryofixation artifacts due to excessive hydration of the skin. The corresponding autoradiographs showed strong redistribution of ³H-estradiol. Thus, the visualization method under investigation has its limitations regarding the hydration level of the skin.

show abstract

Section: Sample Preparationmentioning

confidence: 99%

Validation of Freeze-Drying to Visualize Percutaneous <sup>3</sup>H-Estradiol Transport: The Influence of Skin Hydration on the Efficacy of the Method

Neelissen¹,

Arth

Schrijvers

et al. 1998

Skin Pharmacol Physiol

Self Cite

View full text Add to dashboard Cite

show abstract

“…After 2 weeks of exposure, the slides were developed in a Kodak D 19 developer for 4 min. and fixed in Kodak rapid fix for 5 min and rinsed in running deionized water [12][13][14], Slides were then stained with toluidine blue.…”

Section: A Utoradiographymentioning

confidence: 99%

“…Then the samples were treated with propylenoxidc, propylcneoxide and spurr medium (1:1), and embedded in spurr medium. Poly merization took place for 24 h at 70"C [12][13][14],…”

Section: Sample Preparationmentioning

confidence: 99%

Development of an Intracutaneous Depot for Drugs

Ritschel¹,

Panchagnula²,

Stemmer³

et al. 1991

Skin Pharmacol Physiol

View full text Add to dashboard Cite

In previous studies we reported that the permeation of dexamethasone and hydrocortisone across the skin was decreased and penetration into the skin was increased in the presence of Transcutol® (TC) when compared to water as a solvent. The objective of this investigation was to study the effect of TC on the binding of dexamethasone and hydrocortisone to the skin, and on the accumulation and retention in the skin. Adsorption and desorption studies were conducted for dexamethasone and hydrocortisone with full thickness skin (FTS), and epidermis.The amount of dexamethasone and hydrocortisone adsorbed and desorbed with FTS and epidermis was essentially the same. In the presence of TC the amount of dexamethasone and hydrocortisone adsorbed was increased by 100% with both FTS and epidermis, whereas there was no difference in the amount of dexamethasone and hydrocortisone desorbed. A topical delivery system was developed with and without TC and was evaluated in vivo using the rat as an animal model for hydrocortisone accumulation after multiple dosing. The systemic body burden was reduced by 70% and skin retention of hydrocortisone was incresed by 100% in all the layers of the skin. The detection of hydrocortisone accumulation and retention were studied by autoradiography and electron microscopy, and the results support the hydrocortisone depot in the skin due to TC.

show abstract

“…The application of high-resolution EM autoradiography in skin localization studies is rather rare (de Haan et al, 1989;Squier and Lesch, 1988). Based on the highest resolutions described in EM autoradiography literature, de Haan et al (1989) concluded that the technique would make it possible to discriminate between inter-and transcellular drug transport across stratum corneum.…”

Section: Discussionmentioning

confidence: 99%

“…Based on the highest resolutions described in EM autoradiography literature, de Haan et al (1989) concluded that the technique would make it possible to discriminate between inter-and transcellular drug transport across stratum corneum. High-resolution LM autoradiography will suffice too, considering that corneocytes are about 400-600 nm thick, the intercellular domain is about 100-140 nm wide, and the HD value for a 25-nm-thick radioactive line source is 196 nm (Ginsel et al, 1979).…”

Section: Discussionmentioning

confidence: 99%

Reflection contrast microscopy for high resolution detection of3H-estradiol in ultrathin sections of human stratum corneum

Neelissen

Schrijvers

Junginger

et al. 1999

Microsc. Res. Tech.

Self Cite

View full text Add to dashboard Cite

Visualizing drug transport across stratum corneum: cryotechniques, vapour fixation, autoradiography

Cited by 14 publications

References 26 publications

Validation of Freeze-Drying to Visualize Percutaneous <sup>3</sup>H-Estradiol Transport: The Influence of Skin Hydration on the Efficacy of the Method

Validation of Freeze-Drying to Visualize Percutaneous <sup>3</sup>H-Estradiol Transport: The Influence of Skin Hydration on the Efficacy of the Method

Development of an Intracutaneous Depot for Drugs

Reflection contrast microscopy for high resolution detection of3H-estradiol in ultrathin sections of human stratum corneum

Contact Info

Product

Resources

About