2024
DOI: 10.1093/nar/gkae879
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Visualization of Type IV-A1 CRISPR-mediated repression of gene expression and plasmid replication

Mariana Sanchez-Londono,
Selina Rust,
Rogelio Hernández-Tamayo
et al.

Abstract: Type IV CRISPR–Cas (clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins) effector complexes are often encoded on plasmids and are proposed to prevent the replication of competing plasmids. The Type IV-A1 CRISPR–Cas system of Pseudomonas oleovorans additionally harbors a CRISPR RNA (crRNA) that tightly regulates the transcript levels of a chromosomal target and represents a natural CRISPR interference (CRISPRi) tool. This study investigates CRISPRi effects of this system usi… Show more

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