Visualization of the radicle within tee axis of developing and germinating Brassica napus L. embryos

Dubrovsky, Joseph G.; Tykarska, Teresa

doi:10.1016/0098-8472(94)e0010-2

Cited by 3 publications

(2 citation statements)

References 23 publications

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“…Roots and the delimitation of the radicle and hypocotyl were visualized by staining somatic embryos with 1% Procian Blue MX-R (Serva, Heidelberg, Federal Republic of Germany) in distilled water according to Dubrovsky and Tykarska (1995).…”

Section: Procedures For Morphological Obser Ationmentioning

confidence: 99%

Structure and development of somatic embryos formed inArabidopsis thaliana pt mutant callus cultures derived from seedlings

Recklinghausen¹,

Iwanowska²,

Kieft³

et al. 2000

Protoplasma

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Summary. Seeds of the Arabidopsis thaliana mutant primordia timing (pt) were germinated in 2,4-dichlorophenoxyacetic acidcontaining liquid medium. The seedlings formed somatic embryos and nonembryogenic and embryogenic callus in vitro in a time period of approximately two to three weeks. Embryogenesis and callus formation were monitored with respect to origin, structure, and development. Ten days after germination globular structures appeared in close vicinity of and on the shoot apical meristem (SAM). Somatic embryos formed either directly on the SAM region of the seedling or indirectly on embryogenic callus that developed at the SAM zone. Globular structures developed along the vascular tissue of the cotyledons as well, but only incidentally they formed embryos. Upon deterioration, the cotyledons formed callus. Regular subculture of the ernbryogenic callus gave rise to high numbers of somatic embryos. Such primary somatic embryos, grown on callus, originated from meristematic cell clusters located under the surface of the callus. Embryos at the globular and heart-shape stage were mostly hidden within the callus. Embryos at torpedo stage appeared at the surface of the callus because their axis elongated. Secondary somatic embryos frequently formed directly on primary ones. They preferentially emerged from the SAM region of the primary somatic embryos, from the edge of the cotyledons, and from the hypocotyl.We conclude that the strong regeneration capacity of the pt mutant is based on both recurrent and indirect embryogenesis.

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Section: Procedures For Morphological Obser Ationmentioning

confidence: 99%

Structure and development of somatic embryos formed inArabidopsis thaliana pt mutant callus cultures derived from seedlings

Recklinghausen¹,

Iwanowska²,

Kieft³

et al. 2000

Protoplasma

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show abstract

“…The boundaries of the hypocotyl-radicle transition zone were established in a preliminary germination test that was prolonged until the development of collet (transition-zone) hairs, following the markers described previously by Sliwinska et al (2009Sliwinska et al ( , 2012. As is generally accepted for angiosperms, the hypocotyl-radicle boundary in the mature embryo coincides with the basal cap boundary (Esau, 1965;Dubrovsky and Tykarska, 1995). After marking this region with a waterproof pen, its extension during germination and early seedling growth was observed.…”

Section: Experimental Materialsmentioning

confidence: 99%

Endoreduplication in the germinating embryo and young seedling is related to the type of seedling establishment but is not coupled with superoxide radical accumulation

Rewers

Śliwińska

2014

Journal of Experimental Botany

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During germination, the embryo axis elongates and the radicle emerges through the surrounding structures of the seed. However, this elongation is not even along the axis, and it has been suggested that the region responsible for radicle protrusion is related to the type of subsequent seedling establishment. Eleven epigeal- and five hypogeal-type species were selected to study endoreduplication, a process coupled with cell elongation, in the radicle, hypocotyl-radicle transition zone, hypocotyl, and cotyledons of dry and germinating seeds, and in seedlings after radicle protrusion. Flow cytometry was used to establish the proportions of nuclei with different DNA contents, the mean C-value, and the (Σ>2C)/2C ratio. Additionally, a nitroblue tetrazolium chloride test was applied to the embryos/seedlings in the dry state and during and after germination to localize superoxide radical (O2(•-)) accumulation, which has been suggested to play a role in cell elongation. Endoreduplication intensity varied in different species, in the embryo/seedling regions, and with the type of seedling establishment. In most of the cases, it was highest in the transition zone of epigeal species and in the hypocotyl in hypogeal species. O2(•-) was invariably produced during germination in the radicle, and additionally in the transition zone at the time of radicle protrusion; thus, it was not coupled with endoreduplication, and most probably played a role in defence against biotic and abiotic environmental stresses. These results provide information to aid in the selection of the most suitable plant material for molecular research on germination and for monitoring seed priming.

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The origin of the basal part of the embryo root in Brassica napus L. and its role in growth of early seedlings

Tykarska

Kuraś

1995

Structure and Function of Roots

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Visualization of the radicle within tee axis of developing and germinating Brassica napus L. embryos

Cited by 3 publications

References 23 publications

Structure and development of somatic embryos formed inArabidopsis thaliana pt mutant callus cultures derived from seedlings

Structure and development of somatic embryos formed inArabidopsis thaliana pt mutant callus cultures derived from seedlings

Endoreduplication in the germinating embryo and young seedling is related to the type of seedling establishment but is not coupled with superoxide radical accumulation

The origin of the basal part of the embryo root in Brassica napus L. and its role in growth of early seedlings

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