Visualization of the ocular pulse in the anterior chamber of the mouse eye<i>in vivo</i>using phase-sensitive optical coherence tomography

Peng, Li; Ding, Zhihua; Ni, Yang; Xu, Baisheng; Shen, Yi; Du, Changchun; Jiang, Bo

doi:10.1117/1.jbo.19.9.090502

Cited by 10 publications

(6 citation statements)

References 10 publications

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“…Pulse induced changes were detected by Li et al . in the anterior segment of murine eyes between the cornea and the anterior lens surface 20 . Other approaches detected subtle deformations solely in the posterior eye without the need of a reference surface 19 , 21 .…”

Section: Discussionmentioning

confidence: 99%

“…Within the anterior chamber of the mouse eye, Li et al . described displacements in the range of 180 nm 20 . In this work, absolute displacements in the range of 150 nm at physiological IOP of 15 mmHg to 300 nm at 65 mmHg were measured in the proximity of the ONH, see Fig.…”

Section: Discussionmentioning

confidence: 99%

“…First methods to detect these pulsatile changes in IOP and displacement were demonstrated using pneumotonometry 25 and laser interferometry 26 , 27 , respectively. Recently, Fourier-domain (FD) OCT was used for measuring pulse-induced deformations between the cornea and the retina 28 , within the anterior chamber 20 , 23 , as well as within the posterior eye 19 , 21 . In the past, ocular fundus pulsations were associated with the elasticity and thus the stiffness and rigidity of ocular tissues 29 .…”

Section: Introductionmentioning

confidence: 99%

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Ocular fundus pulsations within the posterior rat eye: Chorioscleral motion and response to elevated intraocular pressure

Augustin

Fialová

Fischak

et al. 2017

Sci Rep

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A multi-functional optical coherence tomography (OCT) approach is presented to determine ocular fundus pulsations as an axial displacement between the retina and the chorioscleral complex in the albino rat eye. By combining optical coherence elastography and OCT angiography (OCTA), we measure subtle deformations in the nanometer range within the eye and simultaneously map retinal and choroidal perfusion. The conventional OCT reflectivity contrast serves as a backbone to segment the retina and to define several slabs which are subsequently used for quantitative ocular pulsation measurements as well as for a qualitative exploration of the multi-functional OCT image data. The proposed concept is applied in healthy albino rats as well as in rats under acute elevation of the intraocular pressure (IOP). The evaluation of this experiment revealed an increased pulsatility and deformation between the retinal and chorioscleral complex while increasing the IOP level from 15 mmHg to 65 mmHg. At IOP levels exceeding 65 mmHg, the pulsatility decreased significantly and retinal as well as choroidal perfusion vanished in OCTA. Furthermore, the evaluation of the multi-parametric experiment revealed a spatial correlation between fundus pulsatility and choroidal blood flow. This indicates that the assessed pulsatility may be a valuable parameter describing the choroidal perfusion.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Ocular fundus pulsations within the posterior rat eye: Chorioscleral motion and response to elevated intraocular pressure

Augustin

Fialová

Fischak

et al. 2017

Sci Rep

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“…Male C57BL6J mice aged postnatal 28 days (P28) underwent baseline retinoscopy refraction and axial length measurement using a custom-built optical coherence tomography (OCT), which has been detailed in [ 19 , 20 ] ( Figure 4 ). Two groups were set in the current study.…”

Section: Methodsmentioning

confidence: 99%

A Head-Mounted Spectacle Frame for the Study of Mouse Lens-Induced Myopia

Feng

et al. 2016

Journal of Ophthalmology

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The mouse model has been widely employed to explore the mysteries of myopia. For now, existing techniques for induction of experimental myopia in mice can be classified into three types: (1) devices directly glued to the fur; (2) devices attached using a combination of glue and sutures; (3) devices attached using a skull-mounted apparatus. These techniques each have its advantages, disadvantages when considering the devices stability, safety, complexity, effectiveness, and so forth. Thus, techniques for myopia induction in mice have yet to be further refined to popularize the applications. In this pilot study, we introduce a new head fixation device named the head-mounted spectacle frame apparatus for the study of mouse lens-induced myopia. Surgical procedures for device attachment were relatively simple and easy to learn in our study. Effective myopia induction was validated by retinoscopy refraction and axial length measurement using optical coherence tomography. In addition, it showed improved compliance and reliable safety when compared to the published methods. The head-mounted spectacle frame apparatus provides a new choice for the study of lens-induced myopia in mouse. It also allows for the use of form deprivation, making it attractive for future experimental mouse myopia trials.

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“…A custom-designed SD-OCT system was built for this study, which has been detailed in [ 29 ]. Briefly, a superluminescent diode (SLD) with a central wavelength of 850 nm and a spectral bandwidth of 100 nm was used as a broadband light source, offering a measured axial resolution of ~4.3 μ m in air.…”

Section: Methodsmentioning

confidence: 99%

Assessment of Full-Eye Response to Osmotic Stress in Mouse Model In Vivo Using Optical Coherence Tomography

et al. 2015

Journal of Ophthalmology

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NaCl based solutions were applied as osmotic stress agents to alter the hydration state of the mouse eye. Full-eye responses to these osmotic challenges were monitored in vivo using a custom-built optical coherence tomography (OCT) with an extended imaging range of 12.38 mm. Dynamic changes in the mouse eye were quantified based on the OCT images using several parameters, including the central corneal thickness (CCT), the anterior chamber depth (ACD), the crystalline lens thickness (LT), the cornea-retina distance (CRD), the iris curvature (IC), and the lens scattering intensity (LSI). Apparent but reversible changes in the morphology of almost all the ocular components and the light transparency of the lens are exhibited. Particularly, the ocular dehydration induced by the hypertonic challenges resulted in a closing of the iridocorneal angle and an opacification of the lens. Our results indicated that the ocular hydration is an important physiological process which might be correlated with various ocular disorders, such as dry eye, cataract, and angle-closure glaucoma, and would affect the biometry and imaging of the eye. OCT uniquely enables the comprehensive study of the dynamic full-eye responses to the ocular hydration in vivo.

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Visualization of the ocular pulse in the anterior chamber of the mouse eyein vivousing phase-sensitive optical coherence tomography

Cited by 10 publications

References 10 publications

Ocular fundus pulsations within the posterior rat eye: Chorioscleral motion and response to elevated intraocular pressure

Ocular fundus pulsations within the posterior rat eye: Chorioscleral motion and response to elevated intraocular pressure

A Head-Mounted Spectacle Frame for the Study of Mouse Lens-Induced Myopia

Assessment of Full-Eye Response to Osmotic Stress in Mouse Model In Vivo Using Optical Coherence Tomography

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