The vortex tube is an energy separation device that separates compressed gas stream into a low and a high temperature stream. Present work reports the flow behavior inside the vortex tube for different commonly used fluids with varied properties like Air, He, N 2 , CO 2 and NH 3. Flow behavior investigation for three-dimensional short straight-diverging vortex tube is done with CFD code (ANSYS 16.0). Different turbulent models, standard k-ε, Realizable k-ε and RNG k-ε are tested. Realizable k-ε model was then used for analysis. Flow behavior of gases with varied multi-atomic number is analyzed and compared with literature. The effect on temperature for N 2 is found to be better, followed by He, CO 2 , Air and NH 3 . Energy separation for N 2 is 46 % higher than all other gases. Energy separation and flow behavior inside vortex tube is analyzed and compared with literature.