Visualization of supramolecular structure of Pluronic F127 micellar hydrogels using cryo-TEM

Silva, Laura C. E. da; Borges, António Carlos; Oliveira, Marcelo Ganzarolli de; Farias, Marcelo Alexandre de

doi:10.1016/j.mex.2020.101084

Cited by 15 publications

(13 citation statements)

References 14 publications

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“…In addition, higher porosity is observed for OEGMA300 15 - b -BuMA 26 - b -DEGMA 13 (80.6 ± 0.5%) compared to the one of Pluronic F127 (72.2 ± 0.5%), which is essential for biomedical applications involving drug encapsulation and release and those that require efficient oxygen and nutrient transport through offering a high level of free void space. It is noteworthy that in the gel formed by Pluronic F127, the micelles are being organized, resulting in rows of micelles (Figure , bottom middle, and Figure S17) as previously observed …”

Section: Resultssupporting

confidence: 75%

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Investigation of the Thermogelation of a Promising Biocompatible ABC Triblock Terpolymer and Its Comparison with Pluronic F127

et al. 2022

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Thermoresponsive polymers with the appropriate structure form physical networks upon changes in temperature, and they find utility in formulation science, tissue engineering, and drug delivery. Here, we report a cost-effective biocompatible alternative, namely OEGMA300 15 - b -BuMA 26 - b -DEGMA 13 , which forms gels at low concentrations (as low as 2% w/w); OEGMA300, BuMA, and DEGMA stand for oligo(ethylene glycol) methyl ether methacrylate (MM = 300 g mol –1 ), n -butyl methacrylate, and di(ethylene glycol) methyl ether methacrylate, respectively. This polymer is investigated in depth and is compared to its commercially available counterpart, Poloxamer P407 (Pluronic F127). To elucidate the differences in their macroscale gelling behavior, we investigate their nanoscale self-assembly by means of small-angle neutron scattering and simultaneously recording their rheological properties. Two different gelation mechanisms are revealed. The triblock copolymer inherently forms elongated micelles, whose length increases by temperature to form worm-like micelles, thus promoting gelation. In contrast, Pluronic F127’s micellization is temperature-driven, and its gelation is attributed to the close packing of the micelles. The gel structure is analyzed through cryogenic scanning and transmission electron microscopy. Ex vivo gelation study upon intracameral injections demonstrates excellent potential for its application to improve drug residence in the eye.

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Section: Resultssupporting

confidence: 75%

“…It is noteworthy that in the gel formed by Pluronic F127, the micelles are being organized, resulting in rows of micelles ( Figure 7 , bottom middle, and Figure S17 ) as previously observed. 88 …”

Section: Resultsmentioning

confidence: 99%

Investigation of the Thermogelation of a Promising Biocompatible ABC Triblock Terpolymer and Its Comparison with Pluronic F127

et al. 2022

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“…The 20% (w/v) PF concentration in P–S hydrogels is an adequate and widely used formulation for use as an injectable hydrogel [ 26 ]. At physiological temperature, the PF copolymer less than 15% (w/v) becomes a sol state and appears as a low-viscosity liquid; however, the 20% PF copolymer exists in a gel state [ 43 ]. When the concentration of PF is too high, side effects exist when used in the body [ 44 ].…”

Section: Resultsmentioning

confidence: 99%

Pluronic F-127/Silk Fibroin for Enhanced Mechanical Property and Sustained Release Drug for Tissue Engineering Biomaterial

et al. 2021

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Herein, an injectable thermosensitive hydrogel was developed for a drug and cellular delivery system. The composite was prepared by facile physical mixing of pluronic F-127 (PF) and silk fibroin (SF) in an aqueous solution. The chemical structure, transparency, viscosity, injectability, degradation kinetic, cumulative release of dexamethasone (Dex), a type of corticosteroid drug, and size distribution of the fabricated hydrogels were characterized. Cytotoxicity of the hydrogels was also studied to verify the biocompatibility of the hydrogels. The addition of a proper amount of SF to PF not only improved the mechanical strength but also decreased the degradation rate which improved the fast rate release of hydrophobic drugs. The cytotoxicity of the hydrogel decreased when SF was added to PF in a proper amount. Overall, the results confirm that the composite of PF and SF can be a promising cell and drug delivery system for future application in tissue engineering and regenerative medicine.

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“…The prepared formulation in the present study had a higher concentration than cmc (MP 1.0 :1.26 wt%, MP 5.0 : 6.30 wt%, MP 10 : 12.6 wt%, respectively, when dissolved in 10 mL), so the micellar structures were observed in cryo-TEM observations. NMR and SAXS results revealed that prepared MP formulations formed micellar structures, and SAXS observation results showed that FCC-packed micelles were obtained [29,30]. Pluronic ® F-127 has a triblock polymer with two hydrophilic tails in the structure.…”

Section: Discussionmentioning

confidence: 96%

Development of Self-Administered Formulation to Improve the Bioavailability of Leuprorelin Acetate

et al. 2022

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In recent years, the development of self-injectable formulations has attracted much attention, and the development of formulations to control pharmacokinetics, as well as drug release and migration in the skin, has become an active research area. In the present study, the development of a lipid-based depot formulation containing leuprorelin acetate (LA) as an easily metabolizable drug in the skin was prepared with a novel non-lamellar liquid-crystal-forming lipid of mono-O-(5,9,13-trimethyl-4-tetradecenyl) glycerol ester (MGE). Small-angle X-ray scattering, cryo-transmission electron microscopy, and nuclear magnetic resonance observations showed that the MGE-containing formulations had a face-centered cubic packed micellar structure. In addition, the bioavailability (BA) of LA after subcutaneous injection was significantly improved with the MGE-containing formulation compared with the administration of LA solution. Notably, higher Cmax and faster Tmax were obtained with the MGE-containing formulation, and the BA increased with increasing MGE content in the formulation, suggesting that LA migration into the systemic circulation and its stability might be enhanced by MGE. These results may support the development of self-administered formulations of peptide drugs as well as nucleic acids, which are easily metabolized in the skin.

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Visualization of supramolecular structure of Pluronic F127 micellar hydrogels using cryo-TEM

Abstract: Highlights Visualization of individual Pluronic® F127 micelles in hydrogels. Supramolecular structure evolution of micellar hydrogels as a function of concentration. Plunge-freezing of high viscosity solutions.

Cited by 15 publications

References 14 publications

Investigation of the Thermogelation of a Promising Biocompatible ABC Triblock Terpolymer and Its Comparison with Pluronic F127

Investigation of the Thermogelation of a Promising Biocompatible ABC Triblock Terpolymer and Its Comparison with Pluronic F127

Pluronic F-127/Silk Fibroin for Enhanced Mechanical Property and Sustained Release Drug for Tissue Engineering Biomaterial

Development of Self-Administered Formulation to Improve the Bioavailability of Leuprorelin Acetate

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