Visualization of non-heme ferric and ferrous iron by highly sensitive non-heme iron histochemistry in the stress-induced acute gastric lesions in the rat

Asano, Yoshiya; Meguro, Reiko; Odagiri, S; Li, Chentai; Iwatsuki, Hiroyasu; Shoumura, Kazuhiko

doi:10.1007/s00418-005-0097-6

Cited by 13 publications

(18 citation statements)

References 38 publications

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“…Furthermore, the Turnbull method cannot visualize the precise distribution of NHF[II] because of the autoxidation of NHF [II] in the process of tissue treatments. In PPIrH and PTIrH, the NHF[III] and NHF [II], which are ionically bound to and chelated by cytoplasmic organic substances, are released as free iron in the acidic perfusate, after which they immediately react with ferrocyanide or ferricyanide ions in the perfusate to form insoluble precipitates (Asano et al 2006;Meguro et al 2007). These methods can prevent the oxidation or reduction of tissue non-heme iron during tissue treatments.…”

Section: Discussionmentioning

confidence: 99%

“…The staining of NHF [II] in ovoid macrophages was observed at inclusion bodies and their peripheral parts. The co-localization of NHF [III] and NHF [II] could indicate the presence of redox-active nonheme iron, which weakly binds to carrier molecules and is easy to be non-enzymatically liberated (Das et al 1997;Asano et al 2006). The redox-active iron ion acts as a catalyst in the Haber-Weiss reaction in the presence of superoxide and hydrogen peroxide to generate hydroxyl radicals, which are known to attack a wide range of cellular constituents, including protein, DNA, and membrane lipids to initiate a free radical chain reaction (Crichton et al 2002;Asano et al 2006).…”

Section: Discussionmentioning

confidence: 99%

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Age-related Accumulation of Non-heme Ferric and Ferrous Iron in Mouse Ovarian Stroma Visualized by Sensitive Non-heme Iron Histochemistry

Asano

2011

J Histochem Cytochem.

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SummarySensitive non-heme iron histochemistry-namely, the perfusion-Perls method and perfusion-Turnbull method-was applied to study the distribution and age-related accumulation of non-heme ferric iron and ferrous iron in mouse ovary. Light and electron microscopic studies revealed that non-heme ferric iron is distributed predominantly in stromal tissue, especially in macrophages. By contrast, the distribution of non-heme ferrous iron was restricted to a few ovoid macrophages. Aged ovaries exhibited remarkable non-heme iron accumulation in all stromal cells. In particular, non-heme ferrous iron level was increased in stromal tissue, suggestive of increased levels of redox-active iron, which can promote oxidative stress. Moreover, intense localization of both non-heme ferric and ferrous iron was observed in aggregated large stromal cells that were then characterized as ceroid-laden enlarged macrophages with frothy cytoplasm. Intraperitoneal iron overload in adult mice resulted in non-heme iron deposition in the entire stroma and generation of enlarged macrophages, suggesting that excessive iron accumulation induced macrophage morphological changes. The data indicated that non-heme iron accumulation in ovarian stromal tissue may be related to aging of the ovary due to increasing oxidative stress. (J Histochem Cytochem 60:229-242, 2012) Keywords non-heme iron, ovary, aging, macrophage, sensitive non-heme iron histochemistry Article

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Age-related Accumulation of Non-heme Ferric and Ferrous Iron in Mouse Ovarian Stroma Visualized by Sensitive Non-heme Iron Histochemistry

Asano

2011

J Histochem Cytochem.

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“…To access the ovaries, the abdominal skin was cut by the scalpel incision along the midline until the pubic bone and ovaries were removed after pushing the skin and appendages. The ovaries were then homogenized and existence as well as the level of iron accumulated in ovarian tissue was determined (11,12) by the light absorption device, iron quantitative detection kits with photometric method and software designed by Pars Azmoon Iran company.…”

Section: Samples Collectionmentioning

confidence: 99%

Accumulation of Iron Oxide Nanoparticle and Conventional Iron Oxide in Rat Ovary and Oxidative Stress Caused by It

Poormoosavi

Morovvati

Varzi

et al. 2018

Iran Red Crescent Med J

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Background: Iron is one of the most essential elements of life that plays a major role in structure of the most cells of body and is potentially toxic and dangerous, because it easily participates in oxidation-reduction reactions and produces reactive oxygen species, which leads to oxidative stress; nanomaterials such as iron nanoparticles found in environmental pollution and can also be dangerous. Objectives:The current study aimed at evaluating and comparing the effects of accumulation of conventional iron oxide and iron oxide nanoparticles in rat ovary and its relationship with serum oxidative stress. Methods:The current experimental study was conducted on 5 groups of female rats, control, iron oxide (15 mg/kg), and iron oxide nanoparticles (5, 15, and 45 mg/kg). All rats were treated intraperitoneally for 16 days. Then, they were euthanized and their ovarian tissue was removed, and iron accumulation in the ovaries was measured by atomic absorption. Malondialdehyde (MDA), carbonyl protein, thiol protein, and total antioxidant activity were also measured in rats serum samples.Results: According to the current study findings, iron accumulation increased significantly (P = 0.046) in the group that received conventional iron oxide, as compared with the control group. Also, the mean of total antioxidant activity (4.4 ± 294.31 µM/L) and thiol protein (3.3 ± 381.09 µm/mL) showed a significant (P ≤ 0.05) differences in group that received conventional iron oxide, compared with the other groups, MDA and protein carbonyl had no significant (P ≥ 0.05) difference. Conclusions:According to the findings, conventional iron oxide particle induced more accumulation and more oxidative stress than nanoparticles.

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“…Redox-active non-heme iron catalyzes hydroxyl radical generation through the Haber-Weiss reaction. Asano et al (2006) have used highly sensitive non-heme iron histochemistry to study the distribution of non-heme ferric and ferrous iron (NHF[III] and NHF [II]) in the normal stomach and its changes in the acute gastric lesions induced by restraint water immersion (RWI) stress in the rat. Both NHF[III] and NHF[II] staining increased in the oncotic parietal cells located at the erosive lesion which developed on the gastric mucosal folds after 3 h RWI.…”

Section: Histochemical Techniques In the Investigation Of Diseases Anmentioning

confidence: 99%

The histochemistry and cell biology vade mecum: a review of 2005–2006

Taatjes

Zuber

Roth

2006

Histochem Cell Biol

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The procurement of new knowledge and understanding in the ever expanding discipline of cell biology continues to advance at a breakneck pace. The progress in discerning the physiology of cells and tissues in health and disease has been driven to a large extent by the continued development of new probes and imaging techniques. The recent introduction of semi-conductor quantum dots as stable, specific markers for both fluorescence light microscopy and electron microscopy, as well as a virtual treasure-trove of new fluorescent proteins, has in conjunction with newly introduced spectral imaging systems, opened vistas into the seemingly unlimited possibilities for experimental design. Although it oftentimes proves difficult to predict what the future will hold with respect to advances in disciplines such as cell biology and histochemistry, it is facile to look back on what has already occurred. In this spirit, this review will highlight some advancements made in these areas in the past 2 years.

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Visualization of non-heme ferric and ferrous iron by highly sensitive non-heme iron histochemistry in the stress-induced acute gastric lesions in the rat

Cited by 13 publications

References 38 publications

Age-related Accumulation of Non-heme Ferric and Ferrous Iron in Mouse Ovarian Stroma Visualized by Sensitive Non-heme Iron Histochemistry

Age-related Accumulation of Non-heme Ferric and Ferrous Iron in Mouse Ovarian Stroma Visualized by Sensitive Non-heme Iron Histochemistry

Accumulation of Iron Oxide Nanoparticle and Conventional Iron Oxide in Rat Ovary and Oxidative Stress Caused by It

The histochemistry and cell biology vade mecum: a review of 2005–2006

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