VISUALIZATION OF NEW ULTRASTRUCTURAL COMPONENTS IN THE CELL WALL OF <i>CANDIDA ALBICANS</i> WITH FIXATIVES CONTAINING TAPO

Djaczenko, W.; Cassone, Antonio

doi:10.1083/jcb.52.1.186

Cited by 75 publications

(33 citation statements)

References 16 publications

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“…Although the biosynthesis of the wall of C. albicans is not fully understood, protoplast regeneration studies, electron microscopy and chemical determinations have revealed the chemical composition of the wall. These observations have confirmed the importance of the plasma membrane in the synthesis of cell wall layers (Duran et al, 1975;Tronchin et al, 1984;Marriott, 1977;Djaczenko & Cassone, 1971 ;Korn & Northcote, 1960;Takamiya et al, 1985;Elorza et al, 1983).…”

Section: Introductionsupporting

confidence: 68%

A cell surface/plasma membrane antigen of Candida albicans

Cutler

1991

Journal of General Microbiology

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Antibody from BALB/cByJ mice immunized against a membranous fraction of Candih albicans agglutinated whole cells as well as the membranous fraction. Hybridoma techniques were used to isolate an IgM monoclonal antibody (mAb) designated 1OG which agglutinated whole cells and reacted with the subcellular fraction. Yeast cells of 15 additional C. albicans strains and isolates of C. stellatoidka, C. tropicalis, C. intermedia and C. lusitaniae were also agglutinated by mAb 1OG. The antigen was not detected on other fungi, including Can&& krusei, C.utilis, Cryptococcus neoformans, Cr. albidus, Torulopsis glabrata, Rhodotorula spp. and Saccharomyces cerevisiae.To determine the cellular location of the epitope to which mAb 10G is specific, freeze-substitution was compared with traditional chemical fixation methods in preparation of samples for immunocolloidal gold electron microscopy (IEM). With both fixation procedures, the antigen recognized by mAb 1OG was found randomly and densely concentrated on the plasma membrane on exponential-phase yeast-form cells and had a patchy distribution on the cell wall surface. Association of the antigen with the plasma membrane was confirmed by IEM of isolated membranes. On developing hyphal cells, antigen appeared first on the plasma membrane and later on the cell wall

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Section: Introductionsupporting

confidence: 68%

A cell surface/plasma membrane antigen of Candida albicans

Cutler

1991

Journal of General Microbiology

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“…4. In keeping with the more drastic extraction procedure and the consequent removal of wall materials, the overall wall thickness is markedly reduced, from about 135 nm in Alk-1 cells to about 85 nm', a value roughly corresponding to the thickness of the inner layers of the untreated wall (Figs 4, 8c and Table 3, see also Djaczenko & Cassone, 1972). More striking, however, is the total loss of layering apparently caused by the complete removal of the electron-dense components (Figs 4 and 8 c).…”

Section: Alkali Extraction Con A-agglutination and Wall Ultrastructurementioning

confidence: 99%

Agglutination of Blastospores of Candida albicans by Concanavalin A and its Relationship with the Distribution of Mannan Polymers and the Ultrastructure of the Cell Wall

Cassone¹,

Mattia²,

Boldrini³

1978

Journal of General Microbiology

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263Blastospores of Candida albicans were readily agglutinated by Concanavalin A (Con A) owing to the specific binding of this lectin to the mannan receptors of the cell surface. When mannan was extracted from the cell wall by neutral buffers, alkali and acid, the agglutination was decreased or lost depending on the degree of extraction. A relatively mild alkali treatment was sufficient to derange the multilayered wall organization and transform it into a uniform, medium-density structure having about the same thickness as the untreated wall. After a more drastic extraction, all the electron-dense components of the wall were lost, the residual, alkali-insoluble wall fabric being completely electron-transparent and of about the same thickness as the inner wall region of untreated cells.Thiol-reducing agents like mercaptoethanol or dithiothreitol also extracted wall materials, an effect which was enhanced by pronase. After dithiothreitol-pronase treatment, the outer wall layers were removed but the inner wall region was not apparently damaged and some electron-dense components remained. None of these treatments significantly affected blastospore agglutination by Con A -this was reduced (but not abolished) only by the sequential action of pronase and helicase, which led to sphaeroplast formation. These sphaeroplasts showed a varied amount of residual wall consisting of evenly distributed, fibrogranular components.Two main conclusions were drawn from these results: (i) mannan polymers extend throughout the wall of the blastospore of C. albicans; (ii) the layering of the wall, as seen by ordinary fixation and staining for electron microscopy, essentially reflects the distribution of the various alkali-soluble complexes, at different levels, both over and in the rigid, glucan-chitin matrix.

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“…The walls are composed mostly of mannoproteins and fibrous and branched glycans that link other components of the wall such as chitin, as demonstrated by biochemical and degradative techniques. Carbohydrate-containing molecules have been located on the yeast cell wall by ultrastructural cytochemistry (2,5,6,8,16,20,28,30,31,48,56).…”

Section: Discussionmentioning

confidence: 99%

A cytochemical study of acid carbohydrates on the surface of Candida lipolytica grown in tween 80-containing medium

Nascimento¹,

Shariá²,

Lima³

et al. 2000

Braz. J. Microbiol.

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Carbohydrate-containing molecules were located on the surface of Candida lipolytica by using ruthenium red in a cytochemical study. The yeast was grown in media containing Tween 80. The surfactant, at 1.0% and 0.5%, was added to the culture medium in different intervals of time, correspondent to the beginning of exponential growth phase, mid of logarithimic phase and beginning of stationary growth phase. Control cultures were grown in a medium containing glucose. The growth of the yeast in media containing glucose and Tween 80 induced changes in the pattern of distribution and location of acid polyssacharides in the cell wall of the microorganism. In adition, the pattern also changed according to Tween 80 concentration. The influence of Tween 80 on cellular carbohydrate expression is discussed.

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VISUALIZATION OF NEW ULTRASTRUCTURAL COMPONENTS IN THE CELL WALL OF CANDIDA ALBICANS WITH FIXATIVES CONTAINING TAPO

Cited by 75 publications

References 16 publications

A cell surface/plasma membrane antigen of Candida albicans

A cell surface/plasma membrane antigen of Candida albicans

Agglutination of Blastospores of Candida albicans by Concanavalin A and its Relationship with the Distribution of Mannan Polymers and the Ultrastructure of the Cell Wall

A cytochemical study of acid carbohydrates on the surface of Candida lipolytica grown in tween 80-containing medium

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