Visualization of initial bacterial colonization on dentine and enamel in situ

Jung, David Jonathan; Al‐Ahmad, Ali; Follo, Marie; Spitzmüller, Bettina; Hoth-Hannig, Wiebke; Hannig, Matthias; Hannig, Christian

doi:10.1016/j.mimet.2010.03.002

Cited by 55 publications

(74 citation statements)

References 35 publications

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“…Bacterial biofilm formation in the oral cavity starts with an initial phase of microbial adhesion to the pellicle followed by coaggregation and bacterial cell cleavage as well as glucan synthesis Bowen and Koo, 2011]. Despite the wellknown efficacy of fluorides, little is known about their interaction with the in vivo pellicle layer and the impact on initial bioadhesion as a starting point of oral biofilm formation; in situ models offer the opportunity to evaluate the relevant structures extraorally in the adherent state with elaborate methods such as enzyme assays and fluorescence and electron microscopy [Al-Ahmad et al, 2009;Jung et al, 2010]. The aim of this study was to investigate the effect of a customary fluoride solution containing sodium fluoride and amine fluoride on initial bacterial adhesion to enamel and dentin in situ, in direct comparison to chlorhexidine.…”

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confidence: 99%

Effect of Conventional Mouthrinses on Initial Bioadhesion to Enamel and Dentin in situ

et al. 2012

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Aim: The study aimed to investigate the effect of a customary fluoride solution, containing sodium fluoride and amine fluoride, on initial biofilm formation on enamel and dentin in situ compared directly to chlorhexidine. Methods: Bovine enamel and dentin specimens were mounted on maxillary splints carried by 9 subjects. After 1 min of pellicle formation, rinses with tap water (control), chlorhexidine (meridol med CHX 0.2%, GABA) and a fluoride mouthrinse (elmex, GABA) were performed for 1 min. Subsequently, the slabs were carried for another 8 h. The adherent bacteria were determined by DAPI staining, live-dead staining and determination of colony-forming units after desorption; glucan formation was visualized with concanavalin A. Additionally, energy-dispersive X-ray spectroscopy (EDX) analysis of the in situ biofilm layers was conducted, and contact angle measurements were performed. Statistical evaluation was performed by means of the Kruskal-Wallis test followed by the Mann-Whitney U test (p < 0.05). Results: In the control group, significantly higher amounts of adherent bacteria were detected on dentin (4.8 × 10⁶ ± 5.4 × 10⁶ bacteria/cm²) than on enamel (1.2 × 10⁶ ± 1.5 × 10⁶ bacteria/cm², DAPI). Chlorhexidine significantly reduced the amount of adherent bacteria (dentin: 2.8 × 10⁵ ± 3.4 × 10⁵ bacteria/cm²; enamel: 4.2 × 10⁵ ± 8.7 × 10⁵ bacteria/cm²). Rinses with the fluoride solution also significantly reduced bacterial adherence to dentin (8.1 × 10⁵ ± 1.5 × 10⁶ bacteria/cm²). Fluoride could not be detected by EDX analysis of the biofilms. Fluoride mouthrinsing did not influence the wettability of the pellicle-covered enamel surface. Conclusion: In addition to the reduction of demineralization and antibacterial effects, fluorides inhibit initial biofilm formation on dental hard tissues considerably, especially on dentin.

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confidence: 99%

Effect of Conventional Mouthrinses on Initial Bioadhesion to Enamel and Dentin in situ

et al. 2012

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“…It is suggested that we should use RNA-based methods to detect active bacteria in future experiments, although RNA is rapidly degraded and more unstable than DNA. Not only could DAPI rapidly take up into DNA and specificity for the AT-clusters of double-stranded DNA but could also show highly fluorescent nuclei without cytoplasmic fluorescence 29) . DAPI staining could be used to quantify the bacteria in the adherent state on Ti-Cu sintered alloys.…”

Section: Discussionmentioning

confidence: 99%

The anti-bacterial activity of titanium-copper sintered alloy against <i>Porphyromonas gingivalis</i> <i>in vitro </i>

Bai

Zhang

Liu

et al. 2016

Dental Materials Journal

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This study investigates the anti-bacterial property of Ti-Cu sintered alloys against Porphyromonas gingivalis. The anti-anaerobic property of Ti-Cu sintered alloys against P. gingivalis was investigated by antibacterial activity test, DNA measurement, DAPI staining and morphology observation. The antibacterial rates of the Ti-5Cu against P. gingivalis after 18 and 24 h incubation were 36.04 and 54.39%, and those of Ti-10Cu were 68.69 and 75.39%, which were lower than their anti-aerobic abilities. The concentration of P. gingivalis DNA gradually decreased with the increasing Cu content, which was nearly 50% after 24 h incubation on Ti-10Cu. SEM results showed that the shape of P. gingivalis changed and the bacteria broke apart with the addition of Cu and the extension of the culture time. Ti-Cu sintered alloys could not only kill anaerobic bacteria but also reduce the activity of the survived bacteria. The anti-anaerobic mechanism was thought to be in associated with the Cu ion released from Ti-Cu alloy.

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“…DAPI-staining has been successfully used to visualize and quantify initially adherent oral microorganisms in situ (Jung et al, 2010), while FISH is an established fluorescence-based staining method allowing the qualitative and quantitative determination of in situ oral biofilm formation without destroying the natural environment of the adherent oral microorganisms (Zijnge et al, 2010). The efficient exploration of the oral biofilm on bovine enamel and various other biomaterial surfaces is only possible with the aid of such non-destructive visualizing techniques (Karygianni et al, 2012;Schaudinn et al, 2009).…”

Section: à2mentioning

confidence: 99%

“…4¢,6-Diamidino-2-phenylindole (DAPI)-staining has been shown to be an appropriate method for the visualization and quantification of oral microorganisms, which are initially adherent on a variety of implant materials (Al-Ahmad et al, 2013;Jung et al, 2010). Fluorescence in situ hybridization (FISH) has been shown to be a useful staining technique for the detection and visualization of bacteria in initial and mature oral biofilms, and has the advantage of functioning without disrupting the natural biofilm structure (Keijser et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Bacterial adhesion and biofilm formation on yttria-stabilized, tetragonal zirconia and titanium oral implant materials with low surface roughness - an in situ study

Al‐Ahmad

Karygianni

Wartenhorst

et al. 2016

Journal of Medical Microbiology

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Visualization of initial bacterial colonization on dentine and enamel in situ

Cited by 55 publications

References 35 publications

Effect of Conventional Mouthrinses on Initial Bioadhesion to Enamel and Dentin in situ

Effect of Conventional Mouthrinses on Initial Bioadhesion to Enamel and Dentin in situ

The anti-bacterial activity of titanium-copper sintered alloy against <i>Porphyromonas gingivalis</i> <i>in vitro </i>

Bacterial adhesion and biofilm formation on yttria-stabilized, tetragonal zirconia and titanium oral implant materials with low surface roughness - an in situ study

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