Visualization of capture line protein binding in nitrocellulose diagnostic membranes

Wang, Jun; Johannsmann, Diethelm; Hollas, Markus; Thom, Volkmar

doi:10.1016/j.desal.2006.03.056

Cited by 7 publications

(7 citation statements)

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“…The initial spatial concentration profile of ferritin at the test line was assumed to follow a rounded rectangular step function (Planck-taper window function). Justification for this general shape comes from an analysis by Wang and colleagues, whereby fluorescently labeled antibody was striped onto nitrocellulose over a range of conditions …”

Section: Materials and Methodsmentioning

confidence: 99%

“…Justification for this general shape comes from an analysis by Wang and colleagues, whereby fluorescently labeled antibody was striped onto nitrocellulose over a range of conditions. 45 The model was implemented in COMSOL Multiphysics (COMSOL, Inc., Burlington, MA) as a time-dependent simulation, with model inputs as described in Table S1 in the Supporting Information. An optimization routine using the BOBYQA method 46 was added to the model to allow for the determination of kinetic binding coefficients, test line antigen activity, and the optical calibration curve coefficients that best fit model to experimental data.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

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Threshold-Based Quantification in a Multiline Lateral Flow Assay via Computationally Designed Capture Efficiency

et al. 2018

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Lateral flow assays (LFAs) are widely used for yes/no detection of analytes, but they are not well-suited for quantification. We show that the sensitivity of the test line in a lateral flow assay can be tuned to appear at a specific sample concentration by varying the density of capture molecules at the test line and that when test lines tuned for different responses are combined into a single test strip, lines appear at specific thresholds of sample concentration. We also developed a model based on mass-action kinetics that accurately described test line signal and shape over a wide matrix of capture molecules and sample concentrations in single-line strips. The model was used to design a three-line test strip with lines designed to appear at logarithmically spaced sample concentrations, and the experiments showed a remarkable match to predictions. The response of this "graded ladder bar" format is due to the effect of test line concentration on capture efficiency at each test line, not on sample depletion effects, and the effect is maintained whether a system is under kinetic or equilibrium control. These features enable design of nonlinear responses (logarithmic here) and suggest robustness for different systems. Thus, the graded ladder bar format could be a useful tool for applications requiring quantification of sample concentrations over a wide dynamic range.

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Section: Materials and Methodsmentioning

confidence: 99%

Section: ■ Materials and Methodsmentioning

confidence: 99%

Threshold-Based Quantification in a Multiline Lateral Flow Assay via Computationally Designed Capture Efficiency

et al. 2018

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“…Nitrocellulose (NC) membranes, which are a porous hydrophilic paper-like substrate, have been used as the paper-based material in this approach because they have a uniform pore size, smooth surface, and high protein-binding capability, which makes them suitable for protein immobilization. 5,6 Recently, several methods, including photolithography, 7 plasma oxidation, 8 and plotting, 9 have been reported to create a hydrophobic barrier to control the fluid direction or the rate of the fluid absorption on paper. However, each of these methods has some disadvantages.…”

Section: Introductionmentioning

confidence: 99%

Development of automated paper-based devices for sequential multistep sandwich enzyme-linked immunosorbent assays using inkjet printing

et al. 2013

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To the best of our knowledge, this is the first report on paper-based devices for automating the sequential multistep procedures of a sandwich-type enzyme-linked immunosorbent assay (ELISA) that require only a single-step application of the sample solution. The device was based on a piece of nitrocellulose (NC) membrane with specially designed channels, where all the reagents are applied at different locations in order to control the fluid travel to the detection region. The inkjet printing method, a simple and low-cost process, was used to create the flow channel and device barrier patterns. The fabricated barrier was found to be an efficient boundary for the liquid along the printed design in the NC membrane, enabling direct control of the reagent flow time. ELISA results were obtained with a single-step sample application. The developed devices (so-called automated paper-based devices) provided a simple procedure for the sandwich ELISA, while reducing assay time and reagent consumption. Colorimetric results were measured using digital camera imaging with software processing. The capability of the method developed herein was successfully used to determine the levels of human chorionic gonadotropin (hCG) by ELISA.

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“…Lateral flow immunoassays have been widely introduced in the field of food science and healthcare [1,2], due to their sensitivity, rapid testing, inexpensive manufacturing cost and easy to operate procedure [3]. A large number of testings are available for the detection of certain infectious diseases [4], food hygiene [5,6], fertility test and etc.…”

Section: Introductionmentioning

confidence: 99%

Preparation and Characterization of Various Membrane Morphologies for Lateral Flow Immunoassay Development

Ahmad

Low

Shukor

et al. 2017

AMST

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Lateral flow in nitrocellulose membrane is widely used as the transport medium in immunological test strips. The immunoassay performance depends greatly on the binding mechanism on membrane surface. If the membrane structures could be controlled precisely, various kinds of immunological analysis could be performed effectively and accurately. This study is aimed at a quantitative understanding on (1) the sensitivity of protein quantification on membrane surface by using densitometer and (2) comparison of the protein binding ability on different membrane surface pore structures. Concentration of immobilised protein on membrane surface was measured as a function of colour intensity, which is initially stained with Ponceau S. The findings showed that membrane morphologies significantly affect the sensitivity of membrane protein binding ability. Depending on the membrane pore structures, different red spot sharpness and intensities were observed from the stained protein dot. Membrane with smaller pore creates higher protein immobilization density and thus, increases the sensitivity level of the assay. However, larger membrane pore structure is necessary to induce rapid migration rate for faster assay.

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Visualization of capture line protein binding in nitrocellulose diagnostic membranes

Cited by 7 publications

References 0 publications

Threshold-Based Quantification in a Multiline Lateral Flow Assay via Computationally Designed Capture Efficiency

Threshold-Based Quantification in a Multiline Lateral Flow Assay via Computationally Designed Capture Efficiency

Development of automated paper-based devices for sequential multistep sandwich enzyme-linked immunosorbent assays using inkjet printing

Preparation and Characterization of Various Membrane Morphologies for Lateral Flow Immunoassay Development

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