2018
DOI: 10.1158/0008-5472.can-17-2618
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Visualization of Breast Cancer Metabolism Using Multimodal Nonlinear Optical Microscopy of Cellular Lipids and Redox State

Abstract: Label-free nonlinear optical microscopy (NLOM) based on two-photon excited fluorescence (TPEF) from cofactors nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD þ ) is widely used for high-resolution cellular redox imaging. In this work, we combined three label-free NLOM imaging methods to quantitatively characterize breast cancer cells and their relative invasive potential:(ii) coherent Raman scattering of cellular lipids, and (iii) second harmonic generation of extracellular matrix… Show more

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Cited by 28 publications
(18 citation statements)
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“…Fig. 2 ), and assessment of the optical redox ratio of the metabolic coenzymes FAD and NAD(P)H using multiphoton imaging [31] demonstrated a significant decrease in the FAD / [FAD + NAD(P)H] ratio with SIRT3 knock-down in anchorage-independent cells ( Fig. 3C ).…”
Section: Resultsmentioning
confidence: 99%
“…Fig. 2 ), and assessment of the optical redox ratio of the metabolic coenzymes FAD and NAD(P)H using multiphoton imaging [31] demonstrated a significant decrease in the FAD / [FAD + NAD(P)H] ratio with SIRT3 knock-down in anchorage-independent cells ( Fig. 3C ).…”
Section: Resultsmentioning
confidence: 99%
“…Using CARS and SRS microscopy to specifically detect C-H bonds in FAs, we quantify lipid content per cell ( 45 ). Using this method, lipid abundances are measured as the number of pixels that belong to lipid droplets in a cell over the total pixel numbers of the entire cell ( 46 ).…”
Section: Resultsmentioning
confidence: 99%
“…Additionally, a label-free imaging technique called non-linear optical microscopy (NLOM), has been studied for both precision and safety advantages. The principle of label-free non-linear optical microscopy is based on two-photon excited fluorescence (TPEF) from cofactors nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD + ) that provides high-resolution cellular redox imaging (Hou et al, 2018). More interestingly, this technique shares the same redox reactions to CL and UECL, therefore the two techniques could possibly image the tissues simultaneously and complement each other.…”
Section: Conclusion and Future Outlookmentioning
confidence: 99%