Visualization of Blood Vessels without Prior Perfusion

Zwi, L. Jonathan; Sharada, K.; Ormrod, Douglas; Edgar, Stephen

doi:10.1006/mvre.1999.2156

Cited by 2 publications

(2 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…From the tumour biological point of view, the presence of laminin needs further attention. Laminin is an integral part of the basement membrane and has been widely used as an immunohistochemical marker for the basement membrane of blood vessels in tumours and non‐malignant tissues alike15, 16. Recently, several studies suggested that laminin and related extracellular matrix molecules play an essential role in melanoma progression; melanoma cells expressing the laminin‐binding alpha7beta1 integrin show an invasive phenotype17.…”

Section: Discussionmentioning

confidence: 99%

PAS‐positive loops and networks as a prognostic indicator in cutaneous malignant melanoma

Thies

Mangold

Moll

et al. 2001

The Journal of Pathology

View full text Add to dashboard Cite

Recently, microvascular channels, as detected by PAS histochemistry, were positively correlated with poor prognosis in uveal malignant melanoma. Since uveal melanomas are not penetrated by lymphatic vessels, while cutaneous melanomas are, the question arises as to whether these loops and networks are also of prognostic relevance in cutaneous melanoma. Histochemically and immunohistochemically detected loops and networks in 100 cases of cutaneous malignant melanoma were correlated with the occurrence of metastasis in a 10-year follow-up study. To detect these patterns, the significance of various methods (PAS reaction with/without nuclear counterstain, anti-laminin immunohistochemistry) was investigated. The presence of loops and networks was a highly significant prognostic marker (p<0.0001) for metastasis in cutaneous malignant melanoma. The presence of these patterns proved to have higher prognostic relevance for metastasis than Breslow's tumour thickness, especially for stage IB and stage IIA tumours (intermediate thickness/risk). PAS reaction without nuclear counterstain proved to be the best method to detect these patterns. Compared with the conventional staging of Breslow's tumour thickness, and especially so for stage IB and IIA melanomas, the determination of PAS-positive loops and networks in cutaneous malignant melanoma provides additional prognostic information.

show abstract

Section: Discussionmentioning

confidence: 99%

PAS‐positive loops and networks as a prognostic indicator in cutaneous malignant melanoma

Thies

Mangold

Moll

et al. 2001

The Journal of Pathology

View full text Add to dashboard Cite

show abstract

“…They range from transmission electron microscopy (TEM, [3, 10–16]), scanning electron microscopy (SEM, [17–23]), histochemistry [24–27], fluorescence microscopy [6, 28–32], and immunohistochemistry [10, 25, 33–38], to atomic force microscopy [39]. Functional studies were carried out both in vivo and in vitro.…”

Section: Renin Cells In the Context Of The Juxtaglomerular Apparatmentioning

confidence: 99%

Methods for Imaging Renin-Synthesizing, -Storing, and -Secreting Cells

Casellas

2010

International Journal of Hypertension

View full text Add to dashboard Cite

Renin-producing cells have been the object of intense research efforts for the past fifty years within the field of hypertension. Two decades ago, research focused on the concept and characterization of the intrarenal renin-angiotensin system. Early morphological studies led to the concept of the juxtaglomerular apparatus, a minute organ that links tubulovascular structures and function at the single nephron level. The kidney, thus, appears as a highly “topological organ” in which anatomy and function are intimately linked. This point is reflected by a concurrent and constant development of functional and structural approaches. After summarizing our current knowledge about renin cells and their distribution along the renal vascular tree, particularly along glomerular afferent arterioles, we reviewed a variety of imaging techniques that permit a fine characterization of renin synthesis, storage, and release at the single-arteriolar, -cell, or -granule level. Powerful tools such as multiphoton microscopy and transgenesis bear the promises of future developments of the field.

show abstract

Visualization of Blood Vessels without Prior Perfusion

Cited by 2 publications

References 5 publications

PAS‐positive loops and networks as a prognostic indicator in cutaneous malignant melanoma

PAS‐positive loops and networks as a prognostic indicator in cutaneous malignant melanoma

Methods for Imaging Renin-Synthesizing, -Storing, and -Secreting Cells

Contact Info

Product

Resources

About