Visualization of Acidithiobacillus ferrooxidans biofilms on pyrite by atomic force and epifluorescence microscopy under various experimental conditions

Mangold, Stefanie; Laxander, Michaela; Harneit, Kerstin; Rohwerder, Thore; Claus, G.; Sand, Wolfgang

doi:10.1016/j.hydromet.2008.05.044

Cited by 23 publications

(8 citation statements)

References 22 publications

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“…The staining of extracellular polysaccharides of A. ferrooxidans was performed using a lectin technique as previously described by Mangold et al ( 13 ). The technique has been used for the selective staining of EPS of A. ferrooxidans .…”

Section: Methodsmentioning

confidence: 99%

Direct Detection of Fe(II) in Extracellular Polymeric Substances (EPS) at the Mineral-Microbe Interface in Bacterial Pyrite Leaching

et al. 2016

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We herein investigated the mechanisms underlying the contact leaching process in pyrite bioleaching by Acidithiobacillus ferrooxidans using scanning transmission X-ray microscopy (STXM)-based C and Fe near edge X-ray absorption fine structure (NEXAFS) analyses. The C NEXAFS analysis directly showed that attached A. ferrooxidans produces polysaccharide-abundant extracellular polymeric substances (EPS) at the cell-pyrite interface. Furthermore, by combining the C and Fe NEXAFS results, we detected significant amounts of Fe(II), in addition to Fe(III), in the interfacial EPS at the cell-pyrite interface. A probable explanation for the Fe(II) in detected EPS is the leaching of Fe(II) from the pyrite. The detection of Fe(II) also indicates that Fe(III) resulting from pyrite oxidation may effectively function as an oxidizing agent for pyrite at the cell-pyrite interface. Thus, our results imply that a key role of Fe(III) in EPS, in addition to its previously described role in the electrostatic attachment of the cell to pyrite, is enhancing pyrite dissolution.

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Section: Methodsmentioning

confidence: 99%

Direct Detection of Fe(II) in Extracellular Polymeric Substances (EPS) at the Mineral-Microbe Interface in Bacterial Pyrite Leaching

et al. 2016

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“…Combining atomic force microscopy (AFM) with epifluorescence microscopy (EFM) to observe the dynamics and make logical sense of the composition of microbial cell membranes during bioleaching. 131 Of course, the changes in total organic carbon (TOC), polysaccharides, and proteins of EPS layered fractions can also be determined chemically. 132 To comprehend and clarify the EPS variations and the mechanism of bioleaching process, three-dimensional fluorescence spectroscopy was also used to investigate the EPS layered fractions.…”

Section: Microscopic Perspective and Interaction Forcesmentioning

confidence: 99%

Bioleaching of oceanic manganese nodules: Current progress and future prospects

Xue

Feng

et al. 2023

Asia-Pacific J Chem Eng

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The extraction of manganese elements and other strategic elements from oceanic manganese nodules has become a strategic decision to ensure the security of global metal resources and the high‐quality green development of metal resource processing and metallurgy industry due to the gradual depletion of high‐quality terrestrial metal resources and the increase of difficult‐to‐select and difficult‐to‐smelt deposits. In order to achieve the enrichment and separation of diverse elements within oceanic manganese nodules, it is imperative to initiate chemical reactions that facilitate the breakdown of complex oxides, particularly manganese dioxide. Bioleaching is a viable treatment option for oceanic manganese nodules with low operating costs and environmental damage. However, the mechanism of oceanic manganese nodules bioleaching is not fully studied, and the prospect of technology development is uncertain. We have reviewed five influencing factors on bioleaching of oceanic manganese nodules: mineralogical characteristics, chemical reaction equilibrium, thermodynamic characteristics, microbial properties, and operational variables. It then makes new development recommendations and technological system suggestions for the future improvement of oceanic manganese nodule bioleaching, primarily focusing on the development of new technologies for challenging to select and difficult to smelt ores, the practicality of strategic energy metal development, the cell design and its materials revolution, environmental protection and management, and all‐round resource utilization. Solving the above critical technical issues will boost the high‐quality development of metal industry and environmental protection.

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“…23 AFM has been applied to image a variety of biological samples, including 1) macromolecules such as DNA, proteins and biopolymers [145]; 2) bacterial biofilms [146][147][148][149] and 3) bacteria and their appendages [150]. Scanning the sample in air is a simple and suitable approach to observe the ultrastructural appendages of bacterial cells such as the fimbriae and pili.…”

Section: Accepted M Manuscriptmentioning

confidence: 99%

“…B: Vertical deflection image acquired withtapping mode in mineral salt solution. C: EFM image of the same sample location[148].A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT25In the early attempts of imaging the bioleaching bacteria attached on the mineral surface, Gehrke et al[96] obtained an AFM image of A. ferrooxidans cells attached to a defective area of pyrite under contact mode. A. ferrooxidans cells attached on the surface of researchgrade chalcopyrite at different incubation time were imaged in air using tapping mode by Bevilaqua and co-workers [157].…”

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confidence: 99%

A concise review of nanoscopic aspects of bioleaching bacteria–mineral interactions

Diao

Taran

Mahler

et al. 2014

Advances in Colloid and Interface Science

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Bioleaching is a technology for the recovery of metals from minerals by means of microorganisms, which accelerate the oxidative dissolution of the mineral by regenerating ferric ions. Bioleaching processes take place at the interface of bacteria, sulfide mineral and leaching solution. The fundamental forces between a bioleaching bacterium and mineral surface are central to understanding the intricacies of interfacial phenomena, such as bacterial adhesion or detachment from minerals and the mineral dissolution. This review focuses on the current state of knowledge in the colloidal aspect of bacteria-mineral interactions, particularly for bioleaching bacteria. Special consideration is given to the microscopic structure of bacterial cells and the atomic force microscopy technique used in the quantification of fundamental interaction forces at nanoscale.

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Visualization of Acidithiobacillus ferrooxidans biofilms on pyrite by atomic force and epifluorescence microscopy under various experimental conditions

Cited by 23 publications

References 22 publications

Direct Detection of Fe(II) in Extracellular Polymeric Substances (EPS) at the Mineral-Microbe Interface in Bacterial Pyrite Leaching

Direct Detection of Fe(II) in Extracellular Polymeric Substances (EPS) at the Mineral-Microbe Interface in Bacterial Pyrite Leaching

Bioleaching of oceanic manganese nodules: Current progress and future prospects

A concise review of nanoscopic aspects of bioleaching bacteria–mineral interactions

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