Visualization and characterisation of defined hair follicle compartments by Fourier transform infrared (FTIR) imaging without labelling

Lau, Katherine; Hedegaard, Martin A.B.; Kloepper, Jennifer E.; Paus, Ralf; Wood, Bayden Robert; Deckert, Volker

doi:10.1016/j.jdermsci.2011.05.002

Cited by 15 publications

(15 citation statements)

References 59 publications

(86 reference statements)

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“…Fourier‐transform infrared spectroscopy (FTIR) has been used in scalp and hair analysis to produce examples for matching and differentiating single human scalp hairs and to visualise defined hair follicle compartments, in addition to other many applications reported in forensic science . Raman spectroscopy is a powerful laser spectroscopic technique that detects the characteristic vibrational energy levels of a molecule and provides structural and molecular information about the hair sample.…”

Section: Introductionmentioning

confidence: 99%

Conformation changes in human hair keratin observed using confocal Raman spectroscopy after active ingredient application

Essendoubi

Meunier

Scandolera

et al. 2019

Intern J of Cosmetic Sci

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ObjectiveIn hair care cosmetic products’ evaluation, one commonly used method is to evaluate the hair appearance as a gold standard in order to determine the effect of an active ingredient on the final state of the hair via visual appreciation. Although other techniques have been proposed for a direct analysis of the hair fibres, they give only surface or structural information, without any accurate molecular information. A different approach based on confocal Raman spectroscopy has been proposed for tracking in situ the molecular change in the keratin directly in the human hair fibres. It presents a high molecular specificity to detect chemical interactions between molecules and can provide molecular information at various depths at the cortex and cuticle levels.MethodsTo evaluate the potential of confocal Raman spectroscopy in testing the efficiency of cosmetic ingredients on keratin structure, we undertook a pilot study on the effectiveness of a smoothing shampoo on natural human hair, by analysing α‐helix and β‐sheet spectral markers in the Amide I band and spectral markers specific to the cystin sulfur content.ResultsWe confirmed that an active proved to be effective on a gold standard decreases α‐helix keratin conformation and promotes β‐sheet keratin conformation in the hair fibres. We also showed that treatment with the effective active decreases the intensity of covalent disulfide (S–S at 510 cm‐1) cross‐linking bands of cysteine. These data confirm that the effective active also acts on the tertiary structure of keratin.ConclusionFrom these experiments, we concluded that the effective active has a smoothing effect on the human hair fibres by acting on α‐helix and β‐sheet keratin conformation and on the tertiary structure of keratin. Based on these results, confocal Raman spectroscopy can be considered a powerful technique for investigating the influence of hair cosmetic ingredients on keratin structure in human hair fibres. Moreover, this analytical technique has the advantage of being non‐destructive and label free; in addition, it does not require sample extraction or purification and it can be applied routinely in cosmetic laboratories.

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Section: Introductionmentioning

confidence: 99%

Conformation changes in human hair keratin observed using confocal Raman spectroscopy after active ingredient application

Essendoubi

Meunier

Scandolera

et al. 2019

Intern J of Cosmetic Sci

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“…Thus, the IR spectroscopic imaging technique has become a powerful tool in chemical analysis; IR microspectroscopic (IMS) imaging combines spectral and spatial information at the same time and may allow the distribution of vibrational modes on the surface to be visualized. IMS imaging is especially valuable when rough surfaces, such as febrile substrates (e.g., cellulose), are investigated because it also provides information about the surface's topography [17]. IMS imaging can also detect subtle chemical changes in tissues and cells without the use of a staining or a detecting agent [17]; it is a chemical imaging technique that can be applied to a broad range of samples and systems [17]- [19].…”

Section: Introductionmentioning

confidence: 99%

FT-IR Microspectroscopic Imaging of Cross-Sectioned Human Hair during a Bleaching Process

Ryu¹,

Jang²,

Yu³

et al. 2016

JCDSA

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We investigated bleached human hair by FT-IR microspectroscopy and chemical imaging. The cross sectioned hair is approximately 90 μm in diameter, showed cuticle and cortex in chemical imaging. Differential amide I/II absorbance ratio and broadening amide I band between in the cortex and cuticle were confirmed in FT-IR microspectroscopy and chemical imaging. The cystine monoxide band from the products of disulfide oxidation of the amino acid cystine is associated with hair damaging during bleaching process. With increase bleaching time, the band for cystine monoxide shows more intense and larger area in chemical image. The spatially chemical change was investigated in detail by FT-IR microspectroscopy and chemical imaging during the bleached process.

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“…Raw data were imported into Matlab R2011a (MathWorks) and analyzed using the IRootLab algorithm for computational biology and vibrationalmicrospectroscopy within MATLAB [27,28]. Raw data were imported into Matlab R2011a (MathWorks) and analyzed using the IRootLab algorithm for computational biology and vibrationalmicrospectroscopy within MATLAB [27,28].…”

Section: Discussionmentioning

confidence: 99%

“…The spectra obtained by Raman analysis were initially concatenated to the 400 to 1800 cm À1 range and baseline corrected, normalized, and zeroed using LabSpec 6 software. Raw data were imported into Matlab R2011a (MathWorks) and analyzed using the IRootLab algorithm for computational biology and vibrationalmicrospectroscopy within MATLAB [27,28]. Principal component analysis plus linear discriminant analysis (PCA-LDA) is a cascading application that applies LDA to the resultant PCA factors; the quality of information produced depends on the number of PCs employed [7].…”

Section: Discussionmentioning

confidence: 99%

Phenotypic responses in Caenorhabditis elegans following chronic low‐level exposures to inorganic and organic compounds

Mugova

Read

Riding

et al. 2018

Enviro Toxic and Chemistry

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Responses of organisms to sublethal exposure of environmental stressors can be difficult to detect. We investigated phenotypic changes in the tissue of Caenorhabditis elegans via Raman spectroscopy, as well as survival and reproductive output when exposed to chronic low doses of metals (copper, zinc, or silver), an herbicide (diuron), and a pesticide (imidacloprid). Raman spectroscopy measures changes in phenotype by providing information about the molecular composition and relative abundance of biomolecules. Multivariate analysis was used to evaluate the significance of treatment phenotype segregation plots compared with controls. Dose-dependent responses were observed for copper, zinc, silver, and diuron, whereas imidacloprid exposure resulted in a small response over the tested concentrations. Concentration-dependent shifts in nematode biomolecular phenotype were observed for copper. Despite having a dose-dependent reproductive response, silver, diuron, and imidacloprid produced inconsistent biological phenotype patterns. In contrast, there was a clear stepwise change between low concentrations (0.00625-0.5 mg/L) and higher concentration (1-2 mg/L) of ionic zinc. The findings demonstrate that measuring phenotypic responses via Raman spectroscopy can provide insights into the biomolecular mechanisms of toxicity. Despite the lack of consistency between survival and Raman-measured phenotypic changes, the results support the effectiveness of Raman spectroscopy and multivariate analysis to detect sublethal responses of chemicals in whole organisms and to identify toxic effect thresholds. Environ Toxicol Chem 2018;37:920-930. © 2017 SETAC.

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Visualization and characterisation of defined hair follicle compartments by Fourier transform infrared (FTIR) imaging without labelling

Cited by 15 publications

References 59 publications

Conformation changes in human hair keratin observed using confocal Raman spectroscopy after active ingredient application

Conformation changes in human hair keratin observed using confocal Raman spectroscopy after active ingredient application

FT-IR Microspectroscopic Imaging of Cross-Sectioned Human Hair during a Bleaching Process

Phenotypic responses in Caenorhabditis elegans following chronic low‐level exposures to inorganic and organic compounds

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