Visualising recalcitrance by colocalisation of cellulase, lignin and cellulose in pretreated pine biomass using fluorescence microscopy

Donaldson, Lloyd; Vaidya, Alankar A.

doi:10.1038/srep44386

Cited by 55 publications

(44 citation statements)

References 58 publications

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“…Under the repeated collision of grinding medium, lignocellulose effect by the action of impact, shear, compression and friction force, through repeated extrusion, grinding and cold welding process, forming high density dislocations calcium carbonate/lignocelluloses composites in this process. At the same time, the lignocelluloses are gradually refined to nanometer scale, and become ultrafine particles with dispersed distribution, which provides a fast channel for the mutual diffusion of atoms, thus obtaining the synthetic products 27 .…”

Section: Introductionmentioning

confidence: 99%

The properties of fibreboard based on nanolignocelluloses/CaCO3/PMMA composite synthesized through mechano-chemical method

Chen

Cai

Dang

et al. 2018

Sci Rep

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The purpose of this study was to develop a rapid and green method for the synthesis of lignocelluloses-based materials with superior mechanical properties. Samples were produced by hot-pressed method using different concentrations of CaCO3 and poly (methyl methacrylate) particles-filled nanolignocelluloses composites which was synthesized through mechano-chemical method. Poly (methyl methacrylate) and CaCO3 nanoparticles have been used as nanofillers. Bending strength, elasticity modulus, and dimensional stability, thermal properties of the developed lignocelluloses-based composites were determined. In view of the experimental results, it is found that the composites materials have good mechanical, dimensional stability, and thermal properties which enhanced as the filler loading increased. Thus, herein described lignocelluloses-based materials showed important characteristics to be concluded that these composites are suitable to be used for the design of flooring and construction systems.

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Section: Introductionmentioning

confidence: 99%

The properties of fibreboard based on nanolignocelluloses/CaCO3/PMMA composite synthesized through mechano-chemical method

Chen

Cai

Dang

et al. 2018

Sci Rep

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“…From a materials point of view, cell wall micropores provide pathways for water loss during timber drying and for infiltration with chemicals during chemical modification and timber preservative treatments (Skaar, 1988). The size and range of micropores within cell walls also determine the accessibility to enzymes and, thus, have a direct impact on the utilization of cellulosic biomass for biofuel applications and on wood decay (Blanchette et al, 1997;Hill et al, 2005;Chundawat et al, 2011;Pu et al, 2013;Donaldson and Vaidya, 2017). Therefore, studies that provide fundamental knowledge of these porous networks are of significant interest.…”

Section: CMmentioning

confidence: 99%

Comparison of Micropore Distribution in Cell Walls of Softwood and Hardwood Xylem

2018

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The porosity of wood cell walls is of interest for both understanding xylem functionality and from a wood materials perspective. The movement of water in xylem generally occurs through the macroporous networks formed in softwood by bordered pits and in hardwood by the intervessel pits and open conduits created by vessels and perforation plates. In some situations, such as cavitated xylem, water can only move through the micropores that occur in lignified tracheid and fiber cell walls; however, these micropore networks are poorly understood. Here, we used molecular microscopy analysis of radiata pine () and red beech () to determine the distribution of micropores in the secondary walls and middle lamellae of tracheids and fibers in relation to cell wall composition. Using two different types of probe, we identified a greater porosity of secondary cell walls and a reduced porosity of the middle lamella. Areas of reduced porosity were observed in the outer regions of the secondary cell wall of both tracheids and fibers that appear unrelated to lignification or the distribution of cellulose, mannan, and xylan. Hardwood fiber cell walls were less lignified than those of softwood tracheids and showed greater accessibility to porosity probes. Vessel cell walls were comparable to those of fibers in terms of both porosity and lignification. Lignification is probably the primary determinant of cell wall porosity in xylem. The highly lignified middle lamella, and lumen surface, act as a barrier to probe movement and, therefore, water movement in both softwood and hardwood.

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“…Similarly, poor adsorption results in unsuccessful implantation of biomaterials. The interactions at the molecular level that regulate enzymatic adsorption to biomass are not completely understood (Taegtmeyer 2012;Jalak and Väljamäe 2014;Donaldson and Vaidya 2017;Bonnin et al 2019). The comparative influences of specific and nonspecific adsorption mechanism of enzymes are dependent on the surface characteristics of the biomass, the provided flow rate and the environmental conditions.…”

Section: Introductionmentioning

confidence: 99%

Interaction of enzymes with lignocellulosic materials: causes, mechanism and influencing factors

Baig

2020

Bioresour. Bioprocess.

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For the production of biofuel (bioethanol), enzymatic adsorption onto a lignocellulosic biomass surface is a prior condition for the enzymatic hydrolysis process to occur. Lignocellulosic substances are mainly composed of cellulose, hemicellulose and lignin. The polysaccharide matrix (cellulose and hemicellulose) is capable of producing bioethanol. Therefore, lignin is removed or its concentration is reduced from the adsorption substrates by pretreatments. Selected enzymes are used for the production of reducing sugars from cellulosic materials, which in turn are converted to bioethanol. Adsorption of enzymes onto the substrate surface is a complicated process. A large number of research have been performed on the adsorption process, but little has been done to understand the mechanism of adsorption process. This article reviews the mechanisms of adsorption of enzymes onto the biomass surfaces. A conceptual adsorption mechanism is presented which will fill the gaps in literature and help researchers and industry to use adsorption more efficiently. The process of enzymatic adsorption starts with the reciprocal interplay of enzymes and substrates and ends with the establishment of molecular and cellular binding. The kinetics of an enzymatic reaction is almost the same as that of a characteristic chemical catalytic reaction. The influencing factors discussed in detail are: surface characteristics of the participating materials, the environmental factors, such as the associated flow conditions, temperature, concentration, etc. Pretreatment of lignocellulosic materials and optimum range of shear force and temperature for getting better results of adsorption are recommended.

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Visualising recalcitrance by colocalisation of cellulase, lignin and cellulose in pretreated pine biomass using fluorescence microscopy

Cited by 55 publications

References 58 publications

The properties of fibreboard based on nanolignocelluloses/CaCO3/PMMA composite synthesized through mechano-chemical method

The properties of fibreboard based on nanolignocelluloses/CaCO3/PMMA composite synthesized through mechano-chemical method

Comparison of Micropore Distribution in Cell Walls of Softwood and Hardwood Xylem

Interaction of enzymes with lignocellulosic materials: causes, mechanism and influencing factors

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