Visualisation of the obligate hydrocarbonoclastic bacteria Polycyclovorans algicola and Algiphilus aromaticivorans in co-cultures with micro-algae by CARD-FISH

Structure and Long-Term Stability of the Microbiome in Diverse Diatom Cultures

“…Several approaches, such as atomic force microscopy and CARD-FISH (fluorescence in situ hybridization combined with catalyzed reporter deposition), have been employed to directly study interactions within the phycosphere ( 17 , 18 ). However, the ephemeral nature of these communities and the ease with which they are disrupted during collection and examination make these efforts extremely technically challenging.…”

Efficiency of benthic diatom-associated bacteria in the removal of benzo(a)pyrene and fluoranthene

“…2b) and strains growing on crude oil (Table 3) were observed in the bacterial community associated to Nitzschia. This suggests that these associated hydrocarbonoclastic bacteria may enhance biodegradation of PAHs in non-axenic cultures, as previously highlighted (Mishamandani et al, 2016;Thompson et al, 2017Thompson et al, , 2018Severin and Erdner, 2019). The degradation of PAHs by microorganisms depends on their molecular weight, hydrophobicity, and water solubility (Haritash and Kaushik, 2009).…”

“…Studies have shown that the bacteria associated with algae, which comprise both algae-attached and free-living bacteria, are specific to algal species, as algae can release strain-dependent organic compounds that are used by specific bacteria (Bagatini et al, 2014). The presence of oil-and hydrocarbon-degrading bacteria within the phycosphere has been reported for dinoflagellates and diatoms (Gutierrez et al, 2012;Mishamandani et al, 2016;Severin et al, 2016;Thompson et al, 2017Thompson et al, , 2018. These associated bacteria can influence the phytoplankton taxon-specific response to hydrocarbon pollution and oil spills (Severin and Erdner, 2019).…”

Protocols for Monitoring Harmful Algal Blooms for Sustainable Aquaculture and Coastal Fisheries in Chile

“…Similarly, the 16S rRNA gene metabarcoding analysis was performed on the same water, and Figure 10 summarizes a list of bacterial genera detected as free-living bacteria in the water sample. How some of those bacteria may be potentially related to the present phytoplankton is currently unknown, but the predominant genus Algiphilus was previously reported as a dinoflagellate-associating phycosphere [ 33 ]. Exhaustive acquisition of phytoplankton 18S- and bacterial 16S-rRNA sequence information during the 4-year project period and data-processing using improved analysis method is expected to give significant insights into the algal-bacterial interaction that have a strong correlation with HAB dynamics.…”