Visual Servoed Three-Dimensional Rotation Control in Zebrafish Larva Heart Microinjection System

Zhuang, Songlin; Lin, Weiyang; Zhong, Jie; Zhang, Gefei; Li, Li; Qiu, Jianbin; Gao, Huijun

doi:10.1109/tbme.2017.2688375

Cited by 51 publications

(5 citation statements)

References 24 publications

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“…Glass micropipets are the main tool for injecting DNA and other compounds in a wide variety of organisms, from cells 22–25 and embryos 26–30 to small animals such as nematodes, 3–8 Drosophila larvae, 31 and zebrafish larvae. 32 , 33 All injection applications require a means of stabilizing the target. Whereas numerous PDMS microdevices have been developed to fulfill this need, 4–32 all of them assume the need for an unobstructed pathway by which the injection pipet reaches the target.…”

Section: Discussionmentioning

confidence: 99%

Microinjection in C. elegans by direct penetration of elastomeric membranes

Lockery

Pop²,

Jussila³

2023

Biomicrofluidics

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The nematode worm C. elegans is widely used in basic and translational research. The creation of transgenic strains by injecting DNA constructs into the worm's gonad is an essential step in many C. elegans research projects. This paper describes the fabrication and use of a minimalist microfluidic chip for performing microinjections. The worm is immobilized in a tight-fitting microchannel, one sidewall of which is a thin elastomeric membrane through which the injection pipet penetrates to reach the worm. The pipet is neither broken nor clogged by passing through the membrane, and the membrane reseals when the pipet is withdrawn. Rates of survival and transgenesis are similar to those in the conventional method. Novice users found injections using the device easier to learn than the conventional method. The principle of direct penetration of elastomeric membranes is adaptable to microinjections in a wide range of organisms including cells, embryos, and other small animal models. It could, therefore, lead to a new generation of microinjection systems for basic, translational, and industrial applications.

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Section: Discussionmentioning

confidence: 99%

Microinjection in C. elegans by direct penetration of elastomeric membranes

Lockery

Pop²,

Jussila³

2023

Biomicrofluidics

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show abstract

“…Glass micropipettes are the main tool for injecting DNA and other compounds in a wide variety of organisms, from cells [13][14][15][16] and embryos [17][18][19][20][21] to small animals such as nematodes [3][4][5][6][7][8] , Drosophila larvae 22 [ref2:16] and zebrafish larvae 23,24 . All injection applications require a means of stabilizing the target.…”

Section: Barriers To Adoptionmentioning

confidence: 99%

Microinjection inC. elegansby direct penetration of elastomeric membranes

Lockery

Pop²,

Jussila³

2022

Preprint

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The nematode worm C. elegans is widely used in basic and translational research. The creation of transgenic strains by injecting DNA constructs into the worm’s gonad is an essential step in many C. elegans research projects. This paper describes the fabrication and use of a minimalist microfluidic chip for performing microinjections. The worm is immobilized in a tight-fitting microchannel one sidewall of which is a thin elastomeric membrane which the injection pipette penetrates to reach the worm. The pipette is neither broken nor clogged by passing through the membrane, and the membrane reseals when the pipette is withdrawn. Rates of survival and transgenesis are similar to those in the conventional method. Novice users found injections using the device easier to learn than the conventional method. The principle of direct penetration of elastomeric membranes is readily adaptable to microinjections in a wide range of organisms including cells, embryos, and other small animal models. It could therefore lead to a new generation of microinjection systems for basic, translational, and industrial applications.

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“…Experiments showed that the successful rotation rate of the z-axis was about 94%, and that of the x-axis rotation was 100%. The system is shown in Figure 7b [77][78][79]. The image-based visual servo is also called the 2D visual servo.…”

Section: Microscopic Visual Servoing Controlmentioning

confidence: 99%

A Review on Microscopic Visual Servoing for Micromanipulation Systems: Applications in Micromanufacturing, Biological Injection, and Nanosensor Assembly

et al. 2019

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Micromanipulation is an interdisciplinary technology that integrates advanced knowledge of microscale/nanoscale science, mechanical engineering, electronic engineering, and control engineering. Over the past two decades, it has been widely applied in the fields of MEMS (microelectromechanical systems), bioengineering, and microdevice integration and manufacturing. Microvision servoing is the basic tool for enabling the automatic and precise micromanipulation of microscale/nanoscale entities. However, there are still many problems surrounding microvision servoing in theory and the application of this technology’s micromanipulation processes. This paper summarizes the research, development status, and practical applications of critical components of microvision servoing for micromanipulation, including geometric calibration, autofocus techniques, depth information, and visual servoing control. Suggestions for guiding future innovation and development in this field are also provided in this review.

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Visual Servoed Three-Dimensional Rotation Control in Zebrafish Larva Heart Microinjection System

Abstract: The developed 3-D rotation control scheme is able to be applied to injection of various organs in zebrafish larva body for different experimental requirements.

Cited by 51 publications

References 24 publications

Microinjection in C. elegans by direct penetration of elastomeric membranes

Microinjection in C. elegans by direct penetration of elastomeric membranes

Microinjection inC. elegansby direct penetration of elastomeric membranes

A Review on Microscopic Visual Servoing for Micromanipulation Systems: Applications in Micromanufacturing, Biological Injection, and Nanosensor Assembly

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