In order to study the intelligent navigation design of fully autonomous UAV (Unmanned Aerial Vehicle) for laser positioning, the laser positioning UAV is mainly discussed. An intelligent navigation design system of fully autonomous UAV based on laser positioning is adopted, which can be used not only for material measurement in large mines and wharfs, but also for mine safety and intelligent mining system. Compared with radar technology, acoustic detection and radio scanning, this method has the characteristics of high efficiency and easy realization, and is more suitable for the detection and positioning of low-speed flying small targets in ultra-low altitude visual range. Finally, the software of the system is programmed, including time, inclination, pendulum, positioning and other measurements. The experimental results are illustrated by flow chart. Laser positioning is designed separately according to the actual needs. The UAV and GPS (Global Position System) positioning sensor system are studied and analysed, and the two are combined to get three-dimensional coordinates. The test results show that the system can basically fulfill the requirement of autonomous range of laser positioning, and provide data support for the later positioning system.