12Tartary buckwheat (Fagopyrum tataricum) is an annual herbaceous crop with a higher content of rutin and 13 flavonoids than other crops. In order to understand the salt tolerance mechanism of Tartary buckwheat, we 14 conducted a transcriptome sequencing study. Two existing and two new salt-tolerant varieties of Tartary 15 buckwheat were used as experimental materials. These varieties were subjected to 100 mM NaCl for 48 16 hours treatment at the 3 leaf stage. Leaf RNA was extracted from 8 samples and performed RNA-seq. We de 17 novo assembled reads into a transcriptome dataset containing 62,637 unigenes with N50 length of 1596 bp. 18A total of 205. 2 million clean reads were obtained, and 33,495 (53.47%) of them were annotated. GO and 19 KEGG classification showed enrichment of these unigenes in 47 sub-categories, 25 KOG functional 20 categories and 129 pathways, respectively. With 11 contrasts, we obtained many differentially expressed 21 genes (DEGs) of transcriptome data in 8 samples. The relative expression of Unigene0009545 and 22Unigene0057231 in M1 and that of Unigene0023096 and Unigene0060321 in M2 was increased significantly 23 under salt stress and may be involved in salt tolerance. These findings will provide valuable reference 24 genetic information for the study on salt tolerance mechanism in plants and assist in cultivating strong 25 salt-tolerant varieties of Tartary buckwheat for greater yield in saline-alkali soil. 26