Visual Electrochemiluminescence Biosensor Chip Based on Distance Readout for Deoxynivalenol Detection

Abstract

Visual electrochemiluminescence (ECL) biosensors do not need complex instruments or well-trained operators, which is regarded as an ideal choice for portable and low-cost detection. But traditional visual ECL biosensors are based on the change in ECL intensity, which is easily affected by environmental factors and signal acquisition processes. In this work, a visual ECL biosensor chip based on distance readout has been developed for the first time. The chip is composed of a square detection region and a visual channel region, which are modified with graphene oxide (GO) and gold nanoparticles (AuNPs)@Ti3C2 nanocomposites, respectively. Target molecules can release aptamers adsorbed on the GO surface of the detection region and further change the electrode potential of the visual channel region, which can determine the length of the long channel that generates visible ECL signals. The application of AuNPs@Ti3C2 nanocomposites can effectively enhance ECL intensity by six times. Through the unique design of the sensor chip, quantification detection can be achieved based on the length change instead of the traditional intensity change. This visual ECL sensor is successfully applied for deoxynivalenol toxin detection in actual samples, demonstrating that the introduction of the distance readout strategy into ECL sensing has a good prospect in on-site testing.

Electrochemiluminescence Microscopy

“…developed an ECL imaging chip on a long‐channel‐shaped working electrode. Target molecules react with aptamers modified on the working electrode, changing the electrode impedance and thus the potential distribution, which determines the region that can generate visible ECL signals [61] …”

Recent Advances in Electrochemiluminescence Biosensors for Mycotoxin Assay

“…Wang’s group developed a visual ECL biosensor chip (2 × 4 cm 2 ) that can convert the concentration of a target into a distance signal ( Figure 10 B) [ 103 ]. The chip device contained a detection region and a distance readout channel region.…”

“… ( A ) Number of publications published each year over the last 10 years on the topic of visual ECL devices. ( B ) Schematic representation of portable ECL sensor for detection of DON (Reproduced with permission from [ 103 ]). ( C ) Amplified visual ECL detection of AFM1 based on a smartphone (Reproduced with permission from [ 88 ]).…”

“…These outstanding features of ECL technique hold great promise in pointof-care diagnosis [101,102]. Figure 10A Wang's group developed a visual ECL biosensor chip (2 × 4 cm 2 ) that can convert the concentration of a target into a distance signal (Figure 10B) [103]. The chip device contained a detection region and a distance readout channel region.…”

A portable polymeric electrochromism-based visual biosensing device with distance readout

“…11 In our earlier work, we have achieved the construction of range-readout biosensing chips based on electrochemiluminescence (ECL) for deoxynivalenol detection. 12 Nevertheless, chemiluminescence usually necessitates additional coreactants and observation within a cartridge setup, thereby restricting its practicality for field testing applications. To address these limitations, here we propose a novel approach that combines naked eye colorimetry with distance signals to develop an electrochromism-based biosensing device for distance readout.…”