Visual detection of pandemic influenza A H1N1 Virus 2009 by reverse-transcription loop-mediated isothermal amplification with hydroxynaphthol blue dye

Visual Detection of High-Risk Human Papillomavirus Genotypes 16, 18, 45, 52, and 58 by Loop-Mediated Isothermal Amplification with Hydroxynaphthol Blue Dye

“…LAMP is being used increasingly for rapid detection and typing of emerging viruses (10,12,20,21). The use of reverse transcription (RT)-LAMP with HNB dye for visual detection of pandemic influenza A H1N1 virus 2009 was developed recently in our laboratory (16).…”

“…Loop-mediated isothermal amplification (LAMP) is a nucleic acid amplification method developed by Notomi et al (18) and has emerged as a powerful gene amplification tool due to its simplicity, speed, specificity, and cost effectiveness. The use of this technique with hydroxynaphthol blue (HNB) dye was first developed in our laboratory and is being used increasingly for rapid and visual detection and typing of emerging viruses (1,16,27).In this study, a simple and visual type-specific LAMP assay for the detection of high-risk HPV genotypes 16, 18, 45, 52, and 58 is described, in which the reaction was carried out in a single tube by mixing primers and DNA polymerase together with the tested samples at 63°C for 65 min. The LAMP assay was further evaluated with HPV DNAs from 294 clinical cervical scrape samples, and the results demonstrate that this assay is sensitive and specific.…”

“…Thus, cervical screening could be improved by testing for the DNA of high-risk types of HPV as a primary screening tool (3,9,24). So far, at least 14 high-risk HPV genotypes have been shown to cause cervical cancer; those most important for cervical cancer are HPV types 16,18,45,31,33,52,58, and 35 (6,7,26). HPV16 is by far the predominant type, causing more than 50% of cancers; HPV18 follows, causing 14%; and HPV45 causes approximately 2.8%.…”

“…Loop-mediated isothermal amplification (LAMP) is a nucleic acid amplification method developed by Notomi et al (18) and has emerged as a powerful gene amplification tool due to its simplicity, speed, specificity, and cost effectiveness. The use of this technique with hydroxynaphthol blue (HNB) dye was first developed in our laboratory and is being used increasingly for rapid and visual detection and typing of emerging viruses (1,16,27).…”

Clinical and Epidemiological Profile of Pandemic Influenza A H1N1, H3N2, and Type B in the Southeast of Caspian Sea, Iran

“…Using real time RT-PCR assay compared with other molecular techniques is recommended for the early diagnosis and the rapid identification of individuals infected with pandemic influenza virus. Fast and accurate detection of influenza improves medical handling by proper conditions of prophylaxis, rapid treatment, and appropriate management plans for public health responses to the outbreaks and the avoidance of unnecessary treatment (8).…”