Visual detection of <i>Fusarium proliferatum</i> based on dual-cycle signal amplification and T5 exonuclease

Wang, Ying; Wang, Xiaoqiang; Gailing, Oliver; Xi, Dongmei

doi:10.1039/d0ra06559e

Cited by 2 publications

(1 citation statement)

References 38 publications

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“…RPA can be applied in low-resource settings, and used as a point-of-care test due to the low operation temperature (25-42°C), and it is time effective (results in 5-20 min) (Daher et al, 2016). Although the method has been used in some fields (detection of pathogens (Zhang et al, 2020;Azinheiro et al, 2022), drug resistance gene (Nakano et al, 2018), and genetically modified crops (Wang et al, 2017(Wang et al, , 2020, it has not been used for anisakid detection.…”

Section: Introductionmentioning

confidence: 99%

Rapid visual detection of anisakid nematodes using recombinase polymerase amplification and SYBR Green I

et al. 2022

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Anisakidosis is a food-borne parasitic disease (FBPD) caused by the third-stage larvae of the family Anisakidae. Therefore, it is important to develop a simple, rapid and equipment-free detection method for anisakids in fish samples or seafood since current methods are time-consuming and require complex instruments. In this study, a recombinase polymerase amplification (RPA)-based method was established for the first time to detect anisakids by targeting the internal transcribed spacer (ITS) regions. The detection results were visualized by including SYBR Green I (SG) in the method. The sensitivity of RPA-SG assay was 102 copies per reaction of recombinant plasmid (within 20 min at 37°C), similar to quantitative real-time PCR (qPCR). The assay had high specificity for detecting anisakids against other related parasites and host fish. In addition, the assay was further used to detect fresh marine fish contaminated with anisakids and it showed high precision. These results indicate that the novel RPA-SG assay suitable for visual detection of anisakids in the field and food safety control.

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Section: Introductionmentioning

confidence: 99%

Rapid visual detection of anisakid nematodes using recombinase polymerase amplification and SYBR Green I

et al. 2022

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A SERS microfluidic chip for ultrasensitive and simultaneous detection of SCCA and CYFRA21-1 in serum based on Au nanobowl arrays and hybridization chain reaction

Cao

et al. 2023

Sensors and Actuators B: Chemical

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Visual detection of Fusarium proliferatum based on dual-cycle signal amplification and T5 exonuclease

Abstract: Schematic illustration of RPA-RCA-assisted dual amplification for visual detection of F. proliferatum.

Cited by 2 publications

References 38 publications

Rapid visual detection of anisakid nematodes using recombinase polymerase amplification and SYBR Green I

Rapid visual detection of anisakid nematodes using recombinase polymerase amplification and SYBR Green I

A SERS microfluidic chip for ultrasensitive and simultaneous detection of SCCA and CYFRA21-1 in serum based on Au nanobowl arrays and hybridization chain reaction

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