Visual detection of cancer cells by colorimetric aptasensor based on aggregation of gold nanoparticles induced by DNA hybridization

Borghei, Yasaman‐Sadat; Hosseini, Morteza; Dadmehr, Mehdi; Hosseinkhani, Saman; Ganjali, Mohammad Reza; Sheikhnejad, Reza

doi:10.1016/j.aca.2015.11.026

Cited by 157 publications

(74 citation statements)

References 30 publications

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Those sensors increase the limits of detection (LOD) by using signal amplifiers but the hybridization times are usually long. [8,9] Despite the advantages, AuNPs are surface-sensitive and may induce undesired aggregations due to high ionic strength and concentration of ions, resulting in false-positive signals.Apart from those approaches, optical detection has drawn substantial attention because of their simplicity, sensitiveness, and multiplexity. The simplicity in design and readout of these sensors had attracted substantial attention.

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confidence: 99%

Upconversion Luminescence Sandwich Assay For Detection of Influenza H7 Subtype

Tsang

Wong

Tsoi

et al. 2019

Adv Healthcare Materials

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Those sensors increase the limits of detection (LOD) by using signal amplifiers but the hybridization times are usually long. [7] On the other hand, colorimetric sensor produce visible color changes upon the presence of the virus genes. The simplicity in design and readout of these sensors had attracted substantial attention. Gold nanoparticles (AuNPs) are one of the promising candidates for such detection strategy because of their high extinction coefficient and aggregation-dependent color change. [8,9] Despite the advantages, AuNPs are surface-sensitive and may induce undesired aggregations due to high ionic strength and concentration of ions, resulting in false-positive signals.Apart from those approaches, optical detection has drawn substantial attention because of their simplicity, sensitiveness, and multiplexity. [10,11] It is the fact that organic dyes are widely used in conventional qPCR assays via downconversion processes. [4,12,13] However, the dyes demonstrate undesirable emissive properties such as poor photostability, broad emission band, and small Stokes shift. [14] Therefore, they were usually employed as quenchers in nanoparticle-based detection systems. [15][16][17] High energy excited quantum dots (QDs) are another representative fluorophores in downconverting assays. [18][19][20] The excitation may induce background fluorescence and broad emission peaks, contributing to severe cross-talking problem. To address these limitations, nearinfrared (NIR) triggered upconversion luminescence (UCL) in lanthanide-doped upconversion nanoparticles (UCNPs) is very promising in biodetection. They not only display large anti-Stokes shift [21,22] but also pose minimal damage to the gene oligos. In addition, the long-lived UCL signal is easily distinguished from the short-lived background signal. As a result, UCNPs were used to detect different oligos and analytes. [23][24][25][26][27][28] The assays involved highly distance-dependent luminescence resonance energy transfer (LRET) between energy donor and acceptor, in which the luminescent intensity changes with the separation between the energy pair. [29] In this work, green emitting UCNPs and AuNPs are selected as the LRET pair owing to their well-matched emission and extinction spectrum, while AuNPs exhibit excellent extinction coefficient and photostability. The size of the nanoparticles played important roles in hybridization [30] because AuNPs demonstrate sizedependent absorption property and it can alter the stability of the hybridized DNA duplex. In addition, the size ratios of the UCNPs and AuNPs affect the formation of UCNPs-AuNPs hybrid structures. Taking advantages of 808 nm excitation of With large anti-Stokes shifts and background-free signals, upconversion luminescent (UCL) screening assays have been a promising method to reduce the transmission of influenza epidemic, which can critically alleviate the disease burden and extra annual deaths. In this work, a luminescent resonance energy transfer sandwich assay is developed, which utilizes core-shell...

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Upconversion Luminescence Sandwich Assay For Detection of Influenza H7 Subtype

Tsang

Wong

Tsoi

et al. 2019

Adv Healthcare Materials

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“…Among various detection methods, colorimetric detection is particularly attractive for its visible change color, easy operation, and rapid reading . Sullivan et al, with aid of 5 / biotinylated primer in PCR amplified Campylobacter jejuni genome and streptavidin‐alkaline phosphatase conjugate and chromogenic substrates, detected quantitate of bacteria in broiler chicken .…”

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confidence: 99%

New Colorimetric DNA Sensor for Detection of Campylobacter jejuni in Milk Sample Based on Peroxidase‐Like Activity of Gold/Platinium Nanocluster

et al. 2019

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A new colorimetric DNA sensor for trace Campylobacter jejuni DNA genome in food samples was investigated. 30 mer DNA probe‐based on hipo gene of Campylobacter jejuni and 12 cytosine nucleotide in tail without any chemical modification was used as the sensing element. 12 cytosine nucleotide utilized as a scaffold for stabilization of Au/Pt nanoclusters. Peroxidase like activity of Au/Pt nanocluster probe with the addition of bacterial target DNA and hybridization process decreased. The experiment showed that step by step increase in bacterial target DNA gradually decreased peroxidase activity, which was linear. Limit of detection for this bacterial DNA sensor was 20 pM and superiority of this DNA sensor for detection of Campylobacter jejuni DNA in comparison with other target DNA was significant. The method was successfully applied to the analysis of bacterial target DNA in milk samples, with results similar to those obtained by the PCR methodology as comparative method.

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“…Basically, the absorption spectra of gold nanoparticles are shifted and the colour of the nanoparticles is changed due to change in their size and aggregation state . The aggregation of Au NPs in the presence of metal ions have great importance for the detection of heavy metal ions in aqueous media.…”

Section: Introductionmentioning

confidence: 99%

Nano Gold Hybrid Polyvinyl Alcohol Films for Sensing of Cu²⁺ ions

et al. 2019

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An efficient and eco‐friendly approach is adopted for the synthesis of highly stable L‐alanine assisted nano gold based polyvinyl alcohol nanocomposite hybrid films (Au/PVA).The –COOH groups of L‐alanine and oxygeneous groups of polyvinyl alcohol are utilized for the synthesis of size controlled gold nanoparticles. The as‐synthesized nanocomposites show rapid and selective detection of Cu2+ ion in aqueous medium. With addition Cu2+ ion to as‐synthesized nanocomposites, the SPR peaks of Au NPs (538 nm) decrease with occurrence of a red shift as well as a new peak is observed at higher wavelength which is due to the aggregation of gold nanoparticles in the nanocomposites hybrid films. Scanning electron microscope (SEM) micrograph and energy dispersive spectroscopy (EDS) reveal the aggregation of gold nanoparticles in the nanocomposite hybrid films. The limit of detection and limit of quantification for Cu2+ ion are 40 nM and 133 nM respectively. This technique for detection of Cu2+ ion in waste water samples is inexpensive and eco‐friendly since no any toxic chemicals are used. In the presence of Cu2+ ion, the colour of the hybrid film is changed hence, this method also involves visual detection of Cu2+ ion without use of electrolyte or transducer. Further, many reported methods represent the in situ monitoring of Cu2+ ion, but the present hybrid film can be used for in situ as well as ex situ monitoring of Cu2+ ion.

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Visual detection of cancer cells by colorimetric aptasensor based on aggregation of gold nanoparticles induced by DNA hybridization

Cited by 157 publications

References 30 publications

Upconversion Luminescence Sandwich Assay For Detection of Influenza H7 Subtype

Upconversion Luminescence Sandwich Assay For Detection of Influenza H7 Subtype

New Colorimetric DNA Sensor for Detection of Campylobacter jejuni in Milk Sample Based on Peroxidase‐Like Activity of Gold/Platinium Nanocluster

Nano Gold Hybrid Polyvinyl Alcohol Films for Sensing of Cu²⁺ ions

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Visual detection of cancer cells by colorimetric aptasensor based on aggregation of gold nanoparticles induced by DNA hybridization

Cited by 157 publications

References 30 publications

Upconversion Luminescence Sandwich Assay For Detection of Influenza H7 Subtype

Upconversion Luminescence Sandwich Assay For Detection of Influenza H7 Subtype

New Colorimetric DNA Sensor for Detection of Campylobacter jejuni in Milk Sample Based on Peroxidase‐Like Activity of Gold/Platinium Nanocluster

Nano Gold Hybrid Polyvinyl Alcohol Films for Sensing of Cu2+ ions

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Product

Resources

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Nano Gold Hybrid Polyvinyl Alcohol Films for Sensing of Cu²⁺ ions