Visual Detection and Two-Dimensional Rotation Control in Zebrafish Larva Heart Microinjection

Zhuang, Songlin; Lin, Weiyang; Zhang, An; Qian, Cheng; Li, Li; Qiu, Jianbin; Gao, Huijun

doi:10.1109/tmech.2017.2717860

Cited by 31 publications

(4 citation statements)

References 30 publications

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“…Experiments showed that the successful rotation rate of the z-axis was about 94%, and that of the x-axis rotation was 100%. The system is shown in Figure 7b [77][78][79]. The image-based visual servo is also called the 2D visual servo.…”

Section: Microscopic Visual Servoing Controlmentioning

confidence: 99%

A Review on Microscopic Visual Servoing for Micromanipulation Systems: Applications in Micromanufacturing, Biological Injection, and Nanosensor Assembly

et al. 2019

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Micromanipulation is an interdisciplinary technology that integrates advanced knowledge of microscale/nanoscale science, mechanical engineering, electronic engineering, and control engineering. Over the past two decades, it has been widely applied in the fields of MEMS (microelectromechanical systems), bioengineering, and microdevice integration and manufacturing. Microvision servoing is the basic tool for enabling the automatic and precise micromanipulation of microscale/nanoscale entities. However, there are still many problems surrounding microvision servoing in theory and the application of this technology’s micromanipulation processes. This paper summarizes the research, development status, and practical applications of critical components of microvision servoing for micromanipulation, including geometric calibration, autofocus techniques, depth information, and visual servoing control. Suggestions for guiding future innovation and development in this field are also provided in this review.

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Section: Microscopic Visual Servoing Controlmentioning

confidence: 99%

A Review on Microscopic Visual Servoing for Micromanipulation Systems: Applications in Micromanufacturing, Biological Injection, and Nanosensor Assembly

et al. 2019

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“…Nevertheless, with the development of robotics and machine vision technology in recent years, significant achievements have been made in automatic microinjection research for zebrafish larvae. Zhuang et al 39 , Qian et al 40 , and Zhang et al 41 . have all achieved automatic microinjection for zebrafish hearts.…”

Section: Introductionmentioning

confidence: 99%

Design of an automated robotic microinjection system for batch injection of zebrafish embryos and larvae

Guo,

Ai,

et al. 2024

Microsyst Nanoeng

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This paper presents the design of a vision-based automated robotic microinjection system for batch injection of both zebrafish embryos and larvae. A novel visual recognition algorithm based on an automatic threshold and excessive dilatation is introduced to accurately identify the center of zebrafish embryos and larval yolks. A corresponding software system is developed using the producer-consumer model as the framework structure, and a friendly user interface is designed to allow operators to choose from a range of desired functions according to their different needs. In addition, a novel microstructural agarose device is designed and fabricated to simultaneously immobilize mixed batches of embryos and larvae. Moreover, a prototype microinjection system is fabricated by integrating hardware devices with visual algorithms. An experimental study is conducted to verify the performance of the robotic microinjection system. The results show that the reported system can accurately identify zebrafish embryos and larvae and efficiently complete batch microinjection tasks of the mixtures with an injection success rate of 92.05% in 13.88 s per sample. Compared with manual and existing microinjection systems, the proposed system demonstrates the merits of versatility, excellent efficiency, high success rate, high survival rate, and sufficient stability.

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“…Although these approaches are functional, they failed to address the issues of friction and low stretchability. This has led to a growing interest in emulating biological features found in nature [25], particularly the human eye's capacity for large deformations in its lens. Inspired by such mechanisms, research on the development of soft tunable reflectors is underway with the advent of smart materials.…”

Section: Introductionmentioning

confidence: 99%

An electromechanically driven dielectric elastomer based tunable reflector

Kashyap,

Agrawal,

Kumar

et al. 2024

Smart Mater. Struct.

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Deformable optics offer numerous advantages over conventional optical assemblies, including compactness, cost-effectiveness, efficiency, and flexibility. This study focuses on a reflector based on Dielectric Elastomer Actuators (DEAs) with an internal fluid (air) coupling. DEAs are a class of electroactive materials adept at accommodating substantial actuation strains and rapid responses. Fluid distributed between the active and passive parts remains entirely enclosed by the device and transmits actuation pneumatically. Dynamic maneuvers conducted through a series of controlled electrical signals demonstrate proper control over optical characteristics. However, DEs exhibit inherent flaws in dynamic actuation, referred to as instabilities, which are mitigated by applying an initial pre-stretch. The study identifies optimal parameters that confer stability to the reflector: minimum to no creep, zero residual vibrations, and low viscous losses. An analytical framework is developed to assess device performance, focusing on the spherical curvature assumption that closely resembles the behavior of tunable spherical reflectors. Additionally, an optical bench setup is employed to demonstrate the relationship between focal length and applied pressure. Notably, this paper underscores the potential of a DE-based variable focal length reflector to function effectively within a dynamic environment.

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Visual Detection and Two-Dimensional Rotation Control in Zebrafish Larva Heart Microinjection

Cited by 31 publications

References 30 publications

A Review on Microscopic Visual Servoing for Micromanipulation Systems: Applications in Micromanufacturing, Biological Injection, and Nanosensor Assembly

A Review on Microscopic Visual Servoing for Micromanipulation Systems: Applications in Micromanufacturing, Biological Injection, and Nanosensor Assembly

Design of an automated robotic microinjection system for batch injection of zebrafish embryos and larvae

An electromechanically driven dielectric elastomer based tunable reflector

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