Visual authenticating hazardous adulterant phenolphthalein in slimming foods: Target-mimicking hapten epitope improved immunoassay

Epitope-Shared Hapten Enhancing Antibody Polyreactivity for Simultaneous Immunochromatography of Oxyphenisatin Adulterants

“…The sensitivity of CG-ICA was assessed using both the cutoff value and the detection capability for screening (CCβ) by previous research . First, standard solutions containing various concentrations of OPHs were fortified into negative jelly, candy tablet, and oral liquid samples.…”

“…Subsequently, according to the Implementing Regulation (EU) 2021/808 of the European Commission, the CCβ of the CG-ICA was determined . CCβ is defined as the lowest analyte concentration detected with a β error probability of ≤5% across 20 distinct samples for each matrix . To achieve this, jelly, candy tablet, and oral liquid matrices, each comprising 20 distinct negative samples, were fortified with the lowest analyte concentration for CG-ICA testing.…”

“…The conformational alignment of the four haptens with OPHs (Figure 2D) revealed varying overlap similarity (Table S2), revealed that the linkers induced slight deflections in the hapten epitopes, which was consistent with findings from our previous study. 22 Among these, hapten OPH-1 demonstrated the highest overlap with similarity values of 0.94/0.76/0.75 for the OPH/OPHA/ OPHP. Furthermore, the ESPs of the shared epitope in four haptens remained consistent with those of the OPHs (Figure 2E).…”

“…The sensitivity of CG-ICA was assessed using both the cutoff value and the detection capability for screening (CCβ) by previous research. 22 First, standard solutions containing various concentrations of OPHs were fortified into negative jelly, candy tablet, and oral liquid samples. Following sample pretreatment, CG-ICA detection was performed to determine the cutoff values, which represent the lowest analyte concentration that produces a positive result.…”

“…23 CCβ is defined as the lowest analyte concentration detected with a β error probability of ≤5% across 20 distinct samples for each matrix. 22 To achieve this, jelly, candy tablet, and oral liquid matrices, each comprising 20 distinct negative samples, were fortified with the lowest analyte concentration for CG-ICA testing. The subsequent assessment involved evaluating the number of positive results: a total of 20 positive outcomes indicated CCβ below the fortified concentration, while 19 positive results indicated that CCβ was equivalent to the fortified concentration.…”