Visual Assay of Total Iron in Human Serum with Bathophenanthrolin Disulfonate-accommodated MCM-41

Sakamoto, Misato; Hizawa, Keita; Hosaka, Manabu; Sugawara, Masao

doi:10.2116/analsci.32.241

Cited by 2 publications

(2 citation statements)

References 26 publications

(22 reference statements)

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“…The speciation of iron (Fe(II)/Fe(III)) is quite important in numerous fields such as plant science, the environment, pharmaceutics, and biology, and an interested reader can find several applications in the international literature [9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25]. A screening of the recently published methods (2016 to date) revealed that the speciation of iron (and/or the determination of total iron) is based on more or less known red/ox pretreatment steps, followed by state-specific color-forming or fluorescent reactions.…”

Section: Introductionmentioning

confidence: 99%

“…An overview of the principles of such procedures can be found in Table 1. As can be seen from the methods included in Table 1, they can be categorized into three main groups: (i) methods that are based on the reduction of Fe(III), typically by ascorbic acid or hydroxylamine [9,10,[12][13][14][15]17,19,20,24]; (ii) methods that are based on the oxidation of Fe(II) by H 2 O 2 [11,16,18]; and (iii) methods where both Fe(II) and Fe(III) interact with the selected probe, offering the possibility to perform total iron quantification in a single run (no speciation is reported though) [21][22][23]. Alternatively, a less-applied recent procedure is the column-based separation of Fe(II)/Fe(III) using cation exchange chromatography followed by compatible ICP detection [25].…”

Section: Introductionmentioning

confidence: 99%

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Speciation of Iron Using Desferal via Simple pH Change and a Single Calibration Curve: High-Throughput Optical Sensor Based on 96-Well Plates and an Overhead Book Scanner as Detector

Baltzis,

Tsiasioti,

Zacharis

et al. 2023

Chemosensors

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In the present study we report the development of an advantageous optical sensor for the speciation of Fe(III)/Fe(II). The sensor is based on the selective reaction of Fe(III) with a Desferal (Deferoxamine) reagent at pH = 2, while both Fe(III) and Fe(II) react with the reagent at pH = 5 using an acetate/glycine buffer. In this way, frequently used extra oxidation (H2O2) or reduction (ascorbic acid or hydroxylamine) steps are avoided. Both species can be determined in the range of 25 to 150 μM using a 96-well plate platform and the instrument-free detection of the colored complex with an overhead book scanner. The LOD is 4 μM, and an additional advantage is that a single calibration curve can be utilized for quantitation. The applicability of the sensor was demonstrated by analyzing commercially available pharmaceutical formulations for quality control purposes.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Speciation of Iron Using Desferal via Simple pH Change and a Single Calibration Curve: High-Throughput Optical Sensor Based on 96-Well Plates and an Overhead Book Scanner as Detector

Baltzis,

Tsiasioti,

Zacharis

et al. 2023

Chemosensors

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A reliable protocol for colorimetric determination of iron oxide nanoparticle uptake by cells

et al. 2017

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Size, shape, and surface properties of superparamagnetic iron oxide nanoparticles (SPIONs) can influence their interaction with biological systems, particularly the incorporation by tumor cells and consequently the biological activity and efficiency in biomedical applications. Several strategies have been used to evaluate cellular uptake of SPIONs. While qualitative methods are generally based on microscopy techniques, quantitative assays are carried out by techniques such as inductively coupled plasma-mass spectrometry and flow cytometry. However, inexpensive colorimetric methods based on equipments commonly found in chemistry and biochemistry laboratories are preferred for routine measurements. Nevertheless, colorimetric assays must be used judiciously, particularly when nanoparticles are involved, since their interaction with biological constituents tends to lead to quite underestimated results. Thus, herein described is a colorimetric protocol using 2,2'-bipyridine as chromogenic ligand, where each step was optimized and validated by total reflection X-ray fluorescence spectroscopy, realizing a highly reproducible and reliable method for determination of iron content in cells incubated with SPIONs. The limit of blank and limit of detection were determined to be as low as 0.076 and 0.143 μg Fe/mL, using sample volumes as small as 190 μL and a number of cells as low as 2.0 × 10. Furthermore, three different types of surface-functionalized nanoparticles were incorporated in cells and evaluated through this protocol, enabling to monitor the additive effect of o-phosphorylethanolamine (PEA) and folic acid (FA) conjugation on iron oxide nanoparticles (SPION-PEA and SPION-PEA/FA), that enhanced the uptake by HeLa cells, respectively, by four and ten times when compared to SPIONs conjugated with nonbioactive molecules. Graphical abstract Colorimetric determination of superparamagnetic iron oxide nanoparticles (SPIONs) incorporated by cells.

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Visual Assay of Total Iron in Human Serum with Bathophenanthrolin Disulfonate-accommodated MCM-41

Cited by 2 publications

References 26 publications

Speciation of Iron Using Desferal via Simple pH Change and a Single Calibration Curve: High-Throughput Optical Sensor Based on 96-Well Plates and an Overhead Book Scanner as Detector

Speciation of Iron Using Desferal via Simple pH Change and a Single Calibration Curve: High-Throughput Optical Sensor Based on 96-Well Plates and an Overhead Book Scanner as Detector

A reliable protocol for colorimetric determination of iron oxide nanoparticle uptake by cells

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