Visual and sensitive detection of viable pathogenic bacteria by sensing of RNA markers in gold nanoparticles based paper platform

Liu, Hongxing; Zhan, Fangfang; Liu, Fang; Zhu, Minjun; Zhou, Xiaoming; Xing, Da

doi:10.1016/j.bios.2014.06.020

Cited by 63 publications

(42 citation statements)

References 56 publications

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“…In another study, Liu et al. reported a lateral flow nucleic acid hybridization assay for the visual detection of isothermally amplified RNA product that was a diagnostic marker for viable Listeria monocytogenes in foodstuff using AuNPs as labels and smartphone imaging as a readout detector [26].…”

Section: Introductionmentioning

confidence: 99%

Ratiometric fluorescence transduction by hybridization after isothermal amplification for determination of zeptomole quantities of oligonucleotide biomarkers with a paper-based platform and camera-based detection

Noor

Hrovat

Moazami-Goudarzi

et al. 2015

Analytica Chimica Acta

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Paper is a promising platform for the development of decentralized diagnostic assays owing to the low cost and ease of use of paper-based analytical devices (PADs). It can be challenging to detect on PADs very low concentrations of nucleic acid biomarkers of lengths as used in clinical assays. Herein we report the use of thermophilic helicase-dependent amplification (tHDA) in combination with a paper-based platform for fluorescence detection of probe-target hybridization. Paper substrates were patterned using wax printing. The cellulosic fibers were chemically derivatized with imidazole groups for the assembly of the transduction interface that consisted of immobilized quantum dot (QD)-probe oligonucleotide conjugates. Green-emitting QDs (gQDs) served as donors with Cy3 as the acceptor dye in a fluorescence resonance energy transfer (FRET)-based transduction method. After probe-target hybridization, a further hybridization event with a reporter sequence brought the Cy3 acceptor dye in close proximity to the surface of immobilized gQDs, triggering a FRET sensitized emission that served as an analytical signal. Ratiometric detection was evaluated using both an epifluorescence microscope and a low-cost iPad camera as detectors. Addition of the tHDA method for target amplification to produce sequences of ∼100 base length allowed for the detection of zmol quantities of nucleic acid targets using the two detection platforms. The ratiometric QD-FRET transduction method not only offered improved assay precision, but also lowered the limit of detection of the assay when compared with the non-ratiometric QD-FRET transduction method. The selectivity of the hybridization assays was demonstrated by the detection of single nucleotide polymorphism.

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Section: Introductionmentioning

confidence: 99%

Ratiometric fluorescence transduction by hybridization after isothermal amplification for determination of zeptomole quantities of oligonucleotide biomarkers with a paper-based platform and camera-based detection

Noor

Hrovat

Moazami-Goudarzi

et al. 2015

Analytica Chimica Acta

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“…Lateral chromatography test strips, whose mechanism is illustrated in Figure 8 , are also widely used. For example, Liu H.X. et al (2014) demonstrated a simple and sensitive method for visual detection of viable pathogenic bacteria based on an isothermal RNA amplification reaction-based bioactive paper-based platform by using a two-dimensional barcode as the receiving/transmitting media for rapid detection.…”

Section: Lateral Chromatography Test Stripsmentioning

confidence: 99%

Aptamer-Based Technologies in Foodborne Pathogen Detection

Teng

Fang

et al. 2016

Front. Microbiol.

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Aptamers are single stranded DNA or RNA ligands, which can be selected by a method called systematic evolution of ligands by exponential enrichment (SELEX); and they can specifically recognize and bind to their targets. These unique characteristics of aptamers offer great potentials in applications such as pathogen detection and biomolecular screening. Pathogen detection is the critical means in detecting and identifying the problems related to public health and food safety; and only the rapid, sensitive and efficient detection technologies can enable the users to make the accurate assessments on the risks of infections (humans and animals) or contaminations (foods and other commodities) caused by various pathogens. This article reviews the development in the field of the aptamer-based approaches for pathogen detection, including whole-cell SELEX and Genomic SELEX. Nowadays, a variety of aptamer-based biosensors have been developed for pathogen detection. Thus, in this review, we also cover the development in aptamer-based biosensors including optical biosensors for multiple pathogen detection by multiple-labeling or label-free models such as fluorescence detection and surface plasmon resonance, electrochemical biosensors and lateral chromatography test strips, and their applications in pathogen detection and biomolecular screening. While notable progress has been made in the field in the last decade, challenges or drawbacks in their applications such as pathogen detection and biomolecular screening remain to be overcome.

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“…Isothermal amplified detection of RNA was employed for the visual and sensitive detection of viable pathogenic bacteria based on a bioactive paper-based platform [92]. The results were transformed into a two-dimensional bar code for a more thorough analysis.…”

Section: Introductionmentioning

confidence: 99%

“…The assay can be employed for the specific detection of 20 CFU/mL of L. monocytogenes in milk and cheese samples. The authors demonstrated that due to its viability, this assay allows for the transmission, reception, and sharing of the results with remote examiners for further testing [92]. …”

Section: Introductionmentioning

confidence: 99%

Development of nanoparticle-based optical sensors for pathogenic bacterial detection

et al. 2017

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BackgroundPathogenic bacteria contribute to various globally important diseases, killing millions of people each year. Various fields of medicine currently benefit from or may potentially benefit from the use of nanotechnology applications, in which there is growing interest. Disease-related biomarkers can be rapidly and directly detected by nanostructures, such as nanowires, nanotubes, nanoparticles, cantilevers, microarrays, and nanoarrays, as part of an accurate process characterized by lower sample consumption and considerably higher sensitivity. There is a need for accurate techniques for pathogenic bacteria identification and detection to allow the prevention and management of pathogenic diseases and to assure food safety.ConclusionThe focus of this review is on the current nanoparticle-based techniques for pathogenic bacterial identification and detection using these applications.

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Visual and sensitive detection of viable pathogenic bacteria by sensing of RNA markers in gold nanoparticles based paper platform

Cited by 63 publications

References 56 publications

Ratiometric fluorescence transduction by hybridization after isothermal amplification for determination of zeptomole quantities of oligonucleotide biomarkers with a paper-based platform and camera-based detection

Ratiometric fluorescence transduction by hybridization after isothermal amplification for determination of zeptomole quantities of oligonucleotide biomarkers with a paper-based platform and camera-based detection

Aptamer-Based Technologies in Foodborne Pathogen Detection

Development of nanoparticle-based optical sensors for pathogenic bacterial detection

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