Visual and On-site Detection of Mercury(II) Ions on Lateral Flow Strips Using DNA-functionalized Gold Nanoparticles

Production of antibody labeled gold nanoparticles for influenza virus H5N1 diagnosis kit development

“…AuNPs are nanomaterials that possess excellent biocompatibility and ease of conjugation to various biological molecules such as peptides, aptamers, antibodies etc. These properties allow colloidal AuNPs to be a prominent candidate for bioassay techniques; examples of such techniques are colorimetric sensor [14][15][16], lateral flow test trip [17][18][19][20], dot blot immunoassay [21][22][23][24], which have been used successfully in biological and medical aims as well. Thus, binding of AuNPs to biomolecules offers a promising approach for facile tracking of desired targets in aqueous samples.…”

A mini-review on functional nucleic acids-based heavy metal ion detection

“…Qualitative detection and semi-quantitative detection were realized by observing the color change or the optical density of the red test line, respectively. As a result, these two strips could detect Hg 2+ with LODs of ~6 nM and 3 nM, within 5 min and 10 min, respectively Yang et al, 2012). In order to improve the sensitivity, Chen et al (2013a) introduced amplification strategies into the construction of dipsticks.…”

Dna功能化金纳米及其应用

“…该法的检测原理是通过生物素/ 链霉亲和素的强相互作用在检测线和对照线上分别 固定不同序列的捕获 DNA 链. AuNP@DNA 能够通过 碱基互补配对固定在对照线上, 而靶标的存在能够 介导部分 AuNP@DNA 探针固定在检测线上, 通过检 测线上固定金纳米颗粒的量能够测定靶标的浓度. 该法简单、便携, 还可以应用于凝血酶 [143] 、铜离 子 [144] 、核酸 [145] 和汞离子 [146] 等靶标的检测. 为了进一步简化修饰步骤、 提高检测的特异性和 灵敏度, Liu 等 [147] 发展了分子信标制备的 AuNP@ DNA 进行核酸的检测.…”