VISLISI trial, a prospective clinical study allowing identification of a new metalloprotease and putative virulence factor from Staphylococcus lugdunensis

Argemi, Xavier; Prévost, Gilles; Riegel, Philippe; Keller, Daniel; Meyer, N.; Baldeyrou, M.; Douiri, N.; Lefèbvre, Nicolas; Meghit, K.; Oustau, C. Ronde; Christmann, D.; Cianférani, Sarah; Strub, Jean‐Marc; Hansmann, Yves

doi:10.1016/j.cmi.2016.12.018

Cited by 33 publications

(46 citation statements)

References 32 publications

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“…Proteolytic activity. We recently described a previously unknown novel protease, named lugdulysin, that might also be implicated in osteoarticular infections (5). This Zn 2ϩ -dependent protease is similar to hyicolysin, another metalloprotease found in S. hyicus, a CoNS isolated from pigs presenting with exudative epidermatitis.…”

Section: Microbiological Studies That Strengthen Clinical Evidencementioning

confidence: 98%

“…Rapid latex and hemagglutination assays have been developed more recently based on the detection of clumping factor, protein A, and capsule types 5 and 8. Nevertheless, these innovative tools fail to distinguish S. aureus from S. lugdunensis efficiently, because S. lugdunensis might produce a bound coagulase (clumping factor) and yield positive results in up to 65% of cases (5). At the biochemical level, S. lugdunensis can be differentiated from other staphylococci based on showing positivity in pyrrolidonyl arylamidase and ornithine decarboxylase reactions in more than 90% of cases (6).…”

Section: S Lugdunensis Identification and Microbiological Issues Havmentioning

confidence: 99%

“…Prospective clinical studies. We recently published the first and currently only prospective study (named VISLISI) (5). In this monocentric clinical trial, all bacteriological samples that yielded positive results for S. lugdunensis were systematically screened during a 3-year period.…”

Section: S Lugdunensis Virulence: Growing Evidences From Clinical Stmentioning

confidence: 99%

“…The authors used the criteria published by Souvenir et al (46), which have proven useful to determine the clinical significance of blood culture positivity for CoNS. Regarding bone, joint, and prosthetic joint infections, the most informative data emerged from the VISLISI clinical trial (5). Among 28 patients with proven infections, 25% had only one positive sample.…”

Section: Time To Change Microbiological and Clinical Criteria Of Intementioning

confidence: 99%

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Is Staphylococcus lugdunensis Significant in Clinical Samples?

et al. 2017

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The implication of coagulase-negative staphylococci in human diseases is a major issue, particularly in hospital settings wherein these species often act as opportunistic pathogens. In addition, some coagulase-negative staphylococci such as have emerged as pathogenic bacteria, implicated in severe infections, particularly, osteoarticular infections, foreign-body-associated infections, bacteremia, and endocarditis. studies have shown the presence of several putative virulence factors such as adhesion factors, biofilm production, and proteolytic factors that might explain clinical manifestations. Taken together, the clinical and microbiological data might change the way clinicians and microbiologists look at in clinical samples.

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Section: Microbiological Studies That Strengthen Clinical Evidencementioning

confidence: 98%

Section: S Lugdunensis Identification and Microbiological Issues Havmentioning

confidence: 99%

Section: S Lugdunensis Virulence: Growing Evidences From Clinical Stmentioning

confidence: 99%

Section: Time To Change Microbiological and Clinical Criteria Of Intementioning

confidence: 99%

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Is Staphylococcus lugdunensis Significant in Clinical Samples?

et al. 2017

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“…lugdunensis is closer to that of S . aureus than that of other CoNS; it may be related to virulence factors such as a fibrinogen binding protein 11 , a von Willebrand factor-binding protein 12 , synergistic hemolysins 13 or a metalloprotease 14 .…”

Section: Introductionmentioning

confidence: 99%

Multiple-Locus Variable Number Tandem Repeat Analysis (MLVA) and Tandem Repeat Sequence Typing (TRST), helpful tools for subtyping Staphylococcus lugdunensis

Dahyot¹,

Lebeurre

François

et al. 2018

Sci Rep

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Staphylococcus lugdunensis is an emergent virulent coagulase-negative Staphylococcus that is increasingly responsible for severe infections. In an attempt to generate informative sequence data for subtyping S. lugdunensis, we selected and sequenced seven polymorphic variable number of tandem repeats (VNTRs) to develop two new methods: a classic length-based multiple-locus VNTR analysis (MLVA) method and a tandem repeat sequence typing (TRST) method. We assessed their performances compared to two existing methods, multilocus sequence typing (MLST) and multivirulence-locus sequence typing (MVLST) for 128 isolates from diverse clinical settings and geographical origins. The clustering achieved by the four methods was highly congruent, with MLVA discriminating within clonal complexes as defined by MLST. Indeed, MLVA was highly discriminant compared to MLST and MVLST in terms of number of genotypes as well as diversity indexes. Sequencing of the seven VNTRs showed that they were stable, and analysis of sequence polymorphisms provided superior discriminatory power. The typeability, reproducibility, and epidemiological concordance of these new methods were excellent. Of note, no link between clustering and clinical settings was identified. This study demonstrates that MLVA and TRST provide valuable information for molecular epidemiological study of S. lugdunensis, and represent promising tools to distinguish between strains of homogenous lineages in this clonal species.

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Comment on the article “Staphylococcus lugdunensis: a neglected pathogen of infections involving fracture-fixation devices”

Argemi

2017

International Orthopaedics (SICOT)

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VISLISI trial, a prospective clinical study allowing identification of a new metalloprotease and putative virulence factor from Staphylococcus lugdunensis

Abstract: This study confirms the pathogenicity of S. lugdunensis, particularly in bone and PJI. We also identified a novel metalloprotease called lugdulysin that may contribute to virulence.

Cited by 33 publications

References 32 publications

Is Staphylococcus lugdunensis Significant in Clinical Samples?

Is Staphylococcus lugdunensis Significant in Clinical Samples?

Multiple-Locus Variable Number Tandem Repeat Analysis (MLVA) and Tandem Repeat Sequence Typing (TRST), helpful tools for subtyping Staphylococcus lugdunensis

Comment on the article “Staphylococcus lugdunensis: a neglected pathogen of infections involving fracture-fixation devices”

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