Visinin-like protein 1 regulates natriuretic peptide receptor B in the heart

Buttgereit, Jens; Qadri, Fatimunnisa; Monti, Jan; Langenickel, Thomas; Dietz, Rainer; Braunewell, Karl-Heinz; Bäder, Michael

doi:10.1016/j.regpep.2009.12.019

Cited by 14 publications

(11 citation statements)

References 43 publications

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“…Common study flaws noted in the reviews included small sample sizes, poor choice of reference standard, poor selection of control population, unclear diagnostic cutoff points, and overall lack of analytical characterization and clinical validation of the proposed biomarkers 15 . VILIP-1 belongs to the family of NCS proteins involved in many physiological processes, such as pain modulation, neuropathological, myocardial processes and carcinogenesis 16,17 . At a molecular level, these proteins play a role in controlling neuronal function.…”

Section: Discussionmentioning

confidence: 99%

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Determination of Serum Visinin Like Protein-1 and Its Potential for the Diagnosis of Brain Injury Due to the Stroke - A Pilot Study

Stejskal¹,

Sporová²,

Švesták³

et al. 2011

BIOMED PAP

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Background. The current diagnosis of stroke relies on clinical examination by a physician supplemented by various neuroimaging techniques. A single set or multiple sets of blood biomarkers that could be used in acute settings to diagnosis stroke, differentiate between stroke types, and ideally predict an initial/recurring stroke would be extremely valuable. The diagnosis of stroke is currently hampered by delay due to lack of a suitable tool for rapid, accurate and analytically sensitive biomarker -based testing. There is a clear need for further assay development and clinical validation in this area (acute stroke setting) in order to improve patient outcomes and quality of life. Visinin like protein 1 (VILIP-1) is a newly discovered CNS-abundant protein which has shown promise in experimental studies, for early stroke diagnosis. However, to date there is no clinical study that has measured VILIP-1 in sera as a marker of stroke.Aim. To develop an assay for the determination of VILIP-1 in human serum, and to investigate its clinical relevance as a marker of ischemic stroke.Design and methods. A new sandwich ELISA was developed, introduced and clinically tested. Mean spiking recovery was 98%. The mean recovery for dilution linearity was 93%. The limit of detection of the assay was 0.01 mcg/l; the intraassay and interassay coefficient of variation (CV) were always less than 10%. The study was approved by the Ethics Commission of the Hospital Šternberk, Czech Republic. A total of 17 healthy individuals (9 men and 8 women, age 64.0 ± 13.0) and 16 individuals with ischemic stroke (10 men and 6 women, age 63.0±11.5) were recruited for our study. The criteria of stroke were proposed by the National Czech Standard. All individuals had blood samples drawn, and VILIP-1 analysis and CT and/or MRI were performed.Results. VILIP-1 serum level significantly differentiated healthy subjects from patients with stroke (P<0.01). All individuals with stroke had VILIP-1 serum values higher than > 0.05 mcg/l, healthy had values below this value. The diagnostic efficacy of serum VILIP-1 was very significant (sensitivity 100%, specificity 100% at 0.093 mcg/l VILIP-1 serum values, AUC 1.0 (CI 0.93-1.0, P<0.01), Chi-squared in the frequency table was 33 (P<0.01).Conclusion. We have introduced a new analytical tool for the study of VILIP-1. Our results support the hypothesis that serum VILIP-1 may be associated with ischemic stroke. The ELISA VILIP-1 assay offers a new research tool for the diagnosis and pathophysiology of stroke and other CNS diseases.

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Section: Discussionmentioning

confidence: 99%

“…VILIP-1 expression has also been described in the heart, and it regulates the Natriuretic receptor B (reduces effect of braun natriuretic peptide) (ref. 16 ). Its overexpression leads to enhanced aChR-dependent inhibitory postsynaptic current generation by interneurons 17 .…”

Section: Discussionmentioning

confidence: 99%

Determination of Serum Visinin Like Protein-1 and Its Potential for the Diagnosis of Brain Injury Due to the Stroke - A Pilot Study

Stejskal¹,

Sporová²,

Švesták³

et al. 2011

BIOMED PAP

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“…However, whether and how NCS-1 or VILIP-2-mediated VGCC regulation contributes to human diseases remain unclear. Conversely, down-regulation of VILIP-1 has been reported in several types of human cancers [106,107] , and in heart failure/cardiac hypertrophy [108] . However, whether VILIP-1 effect is associated with VGCC regulation is unknown.…”

Section: Perspectives and Future Directionsmentioning

confidence: 99%

Calcium binding protein-mediated regulation of voltage-gated calcium channels linked to human diseases

Nejatbakhsh

Feng

2011

Acta Pharmacol Sin

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Calcium ion entry through voltage-gated calcium channels is essential for cellular signalling in a wide variety of cells and multiple physiological processes. Perturbations of voltage-gated calcium channel function can lead to pathophysiological consequences. Calcium binding proteins serve as calcium sensors and regulate the calcium channel properties via feedback mechanisms. This review highlights the current evidences of calcium binding protein-mediated channel regulation in human diseases.

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“…modulate mGC activity [26,27,[42][43][44][45][46]. However, in addition to mGC, each member of the VILIP group is known to interact with multiple targets, generating perhaps the most functionally diverse group among the three [21, 22,25].…”

Section: Tissue Expression Analysismentioning

confidence: 99%

“…Frequenin [44] and HPCA [45] activate the olfactory guanylate cyclase (ONE-GC/GC-D) at elevated calcium concentrations; interestingly, so does GCAP1 [41]. VSNL1, on the other hand, activates the product of NPR2B (atrial natriuretic peptide receptor type B/GC-B) [26,42,43]. The overlapping specificities of NCS proteins to one cyclase, the ability of one NCS protein to modulate different cyclases and opposing responses to elevated calcium are all observed in this interaction, which is spread between two groups and the two outliers.…”

Section: Intricacy Of Ncs/mgc Interactionsmentioning

confidence: 99%

Evolutionary Interrelationships and Insights into Molecular Mechanisms of Functional Divergence: An Analysis of Neuronal Calcium Sensor Proteins

Viviano¹,

Wu²,

Venkataraman³

2013

J Phylogen Evolution Biol

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The normal function of any organism, its organizational complexity notwithstanding, depends on the interaction of its proteins with their targets. Thus, analysis of target site interaction is an essential part of all biology. At the protein level, such analyses are critical to both mechanistic knowledge and potential clinical applications such as drug discovery. Approaches to map amino acid residues involved in target site interaction typically are experimental or are based on three-dimensional structures obtained through crystallography. Here we test a novel approach that combines phylogenetic analyses with mining of experimental data using neuronal calcium sensor proteins. The proteins fall into three groups based on sequence comparison. One interaction was taken up for analysis from each group. Using the sequence divergence to evaluate the role of amino acids identified experimentally to form the interface with the target, we demonstrate that it is possible to predict residues that are likely to contribute to the specificity of the interaction and, therefore, the functional divergence. Thus, evolutionary analyses of proteins provide an important addition in approaches to generate refined maps of target site interactions in proteins. This approach is especially useful in delineating the functional divergence in a family of closely related proteins.

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Visinin-like protein 1 regulates natriuretic peptide receptor B in the heart

Cited by 14 publications

References 43 publications

Determination of Serum Visinin Like Protein-1 and Its Potential for the Diagnosis of Brain Injury Due to the Stroke - A Pilot Study

Determination of Serum Visinin Like Protein-1 and Its Potential for the Diagnosis of Brain Injury Due to the Stroke - A Pilot Study

Calcium binding protein-mediated regulation of voltage-gated calcium channels linked to human diseases

Evolutionary Interrelationships and Insights into Molecular Mechanisms of Functional Divergence: An Analysis of Neuronal Calcium Sensor Proteins

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