Visible light excitable ON fluorescence and naked eye detection of Cu2+via hydrolysis of rhodamine–thiophene conjugate: human breast cancer cell (MCF7) imaging studies

Abstract

Thiophene appended rhodaminehydrazone derivative, RDHDTCA allows selective colorimetric and fluorescence recognition of Cu(2+) as low as 2.4 × 10(-8) M. RDHDTCA is capable to detect intracellular Cu(2+) in human breast cancer cells, MCF7 under fluorescence microscope.

A highly selective fluorescent probe for in vitro and in vivo detection of Hg2+

“…We speculated that it may be caused by the hydrolysis of the probe RS following the mechanism which was shown in the second step of Scheme 2, a similar mechanism has been reported in ref. 46. From these results taken together, a likely sensing mechanism based on the Hg 2+ -triggered spiro ring-opening process is proposed in Scheme 2.…”

Structurally Characterized Antipyrine‐Based Dual Fluorescent Probe: Enhanced AlIII Selectivity of a Dinuclear ZnII Complex for Intracellular Sensing by a Displacement Approach

“…The imaging system was composed of an inverted fluorescence microscope (Dewinter, Italy). Images were captured through an attached CCD camera with the help of BioWizard 4.2 software [28] . The microscope was equipped with a 50 W mercury arc lamp.…”

Detection of human neutrophil elastase (HNE) on wound dressings as marker of inflammation

“…Even so, a second approach by using peptides with specific cleavage sequence for HNE that were coupled to a fluorescence quenching system was also studied. According to the literature, the use of FRET system allows a more sensitive detection of the peptide cleavage in comparison to colorimetric (and even other fluorescence) methods (Lakowicz 2006;Adhikari et al 2014).…”

“…Nowadays, fluorescence systems are widely used in many applications in biotechnology fields, being a method of choice for biological and medical diagnosis due to its inherent sensitivity, selectivity, ease of implementation, response time, spatial resolution and also growing availability of commercial products (Lakowicz 2006;Adhikari et al 2014). A possible limitation for the use of fluorogenic substrates is its direct visual assessment, since the human eye is not a great spectral detector and cannot easily detect fluorescence changes when white light is used as excitation source.…”