Visible Light Affects Chemiluminescence of Carp<i>(Cyprinus carpio)</i>Blood Leukocytes

Belotsky, Sandro; Avtalion, Ramy R.; Sinyakov, Michael S.; Lavi, Ronit; Lubart, Rachel

doi:10.1089/1549541041438632

Cited by 7 publications

(2 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The study concluded that there were no negative side effects after the application of large doses of visible or infrared radiation and considerable systemic responses were exclusively found with ultraviolet irradiation [10]. Belotsky et al [11] studied the effect of visible-light irradiation of 5 J/cm 2 and 12 J/cm 2 applied to buffy coat leukocytes [11]. The study found an inhibitory effect dependent on the light intensity that was more pronounced at 12 J/cm 2 .…”

Section: Laser Chemistrymentioning

confidence: 99%

Changes in Cell Viability of Wounded Fibroblasts following Laser Irradiation in Broad‐Spectrum or Infrared Light

Hawkins

Abrahamse

2006

Laser Chemistry

View full text Add to dashboard Cite

Objective. This study aimed to establish if broad-spectrum or infrared (IR) light in combination with laser therapy can assist phototherapy to improve the cell function of wounded cells. Background. The effect of laser light may be partly or completely reduced by broad-spectrum light. Methods. Wounded human skin fibroblasts were irradiated with 5 J/cm 2 using a helium-neon laser, a diode laser, or an Nd:YAG laser in the dark, in the light, or in IR. Changes in cell viability were evaluated by cell morphology, ATP cell viability, LDH membrane integrity, and caspase 3/7 as an early marker of apoptosis. Results. Wounded cells exposed to 5 J/cm 2 using 632.8 nm in the dark or 830 nm in the light or 1064 nm in the dark showed an increase in ATP viability, an increase in cytokine expression, and a decrease in LDH cytotoxicity indicating that the metabolic activity of the wounded cells was stimulated. Conclusion. Wounded cells irradiated in IR light showed an undesirable thermal effect that was proportional to the duration of exposure.

show abstract

Section: Laser Chemistrymentioning

confidence: 99%

Changes in Cell Viability of Wounded Fibroblasts following Laser Irradiation in Broad‐Spectrum or Infrared Light

Hawkins

Abrahamse

2006

Laser Chemistry

View full text Add to dashboard Cite

show abstract

“…), but some references exist for other mammals (Nagahata et al, 1991;Lojek et al, 1997;Vuotto et al, 2000b;Letellier et al, 2001;McLaren et al, 2003;Khan, 2004;Pavelkova and Kubala, 2004). Limited information is available for other orders of vertebrates, such as fish (Kubala et al, 1996;Belotsky et al, 2004;Nikoskelainen et al, 2004Nikoskelainen et al, , 2006Verho et al, 2005), whereas almost nothing is available about amphibians (Marnila et al, 1995;Gilbertson et al, 2003) and birds (Laudert et al, 1993). Our initial studies for adapting this assay to wild birds were done in chickens because genetic differences are minimal among these inbred birds, and blood samples can be easily collected.…”

Section: Introductionmentioning

confidence: 99%

Validation and Novel Applications of the Whole-Blood Chemiluminescence Assay of Innate Immune Function in Wild Vertebrates and Domestic Chickens

Papp

Smits

2007

Journal of Wildlife Diseases

View full text Add to dashboard Cite

The whole-blood chemiluminescence (WBCL) assay is a simple and rapid method of measuring production of reactive oxygen species by circulating leukocytes, particularly heterophils (birds) and neutrophils (other vertebrates). In the interest of substantiating a broadly applicable measure of innate immunity, we investigated the microplate WBCL method for several wildlife species as well as domestic broiler chickens. Lucigenin as a light enhancer was used for all avian blood, wild and domestic, while luminol was used in bear and frog blood. Use of ethylenedinitrilotetraacetic acid (EDTA) as the anticoagulant caused hemolysis of frog blood and decreased WBCL responses in all animals tested. Heparin, even in high concentrations, caused modest to no decrease of WBCL responses. The WBCL response correlated highly with heterophil or neutrophil numbers in all species tested. The WBCL response was tested in freshly collected blood as well as in blood one to five days postcollection in order to determine the utility of this assay for field studies when immediate access to laboratory facilities is not possible. One to three days of delay in performing the test after blood collection caused no, or only a slight, decay of the chemiluminescence response in most animals. Using domestic chickens, we tested the sensitivity of the WBCL method to detect differences between treatment groups and looked for loss of chemiluminescence response over several days using the original blood samples. Significant differences in the WBCL response between experimental groups of broiler chicken were detectable in freshly collected blood, as well as one- to four-day-old blood. Our results show that the innate immune response of populations of wild animals may be successfully compared using this assay, even when blood cannot be tested until a few days after collection.

show abstract

Vertical and horizontal transmission of tilapia larvae encephalitis virus: The bad and the ugly

et al. 2011

View full text Add to dashboard Cite

Impairment of innate immunity in tilapia larvae after vertical and horizontal infection with the newly characterized tilapia larvae encephalitis virus (TLEV) was accessed by evaluation of cell-mediated reactive oxygen species (ROS) production in affected fish with the use of horseradish peroxidase-amplified luminol-dependent chemiluminescence assay. The priming in-vivo infection with TLEV resulted in downregulation of ROS response in both vertically- and horizontally-infected fish; this suppression was further exacerbated by specific in-vitro booster infection with the same virus. Application of Ca ionophore and phorbol myristate acetate as alternative nonspecific boosters enabled restoration of ROS release in vertically-infected but not in horizontally-infected larvae. The results indicate severe TLEV-imposed phagocyte dysfunction in affected larvae. The difference in restoration potential of ROS production after vertical and horizontal virus transmission is interpreted in the frame of principal distinctions between the two modes.

show abstract

Visible Light Affects Chemiluminescence of Carp(Cyprinus carpio)Blood Leukocytes

Cited by 7 publications

References 21 publications

Changes in Cell Viability of Wounded Fibroblasts following Laser Irradiation in Broad‐Spectrum or Infrared Light

Changes in Cell Viability of Wounded Fibroblasts following Laser Irradiation in Broad‐Spectrum or Infrared Light

Validation and Novel Applications of the Whole-Blood Chemiluminescence Assay of Innate Immune Function in Wild Vertebrates and Domestic Chickens

Vertical and horizontal transmission of tilapia larvae encephalitis virus: The bad and the ugly

Contact Info

Product

Resources

About