Visible Light-Activated Water-Soluble Platicur Nanocolloids: Photocytotoxicity and Metabolomics Studies in Cancer Cells

Abstract

Nanoparticle-based drug delivery systems for cancer therapy offer a great promising opportunity as they specifically target cancer cells, also increasing the bioavailability of anticancer drugs characterized by low water solubility. Platicur, [Pt(cur) (NH 3 ) 2 ](NO 3 ), is a cis-diamine−platinum(II) complex linked to curcumin. In this work, an ultrasonication method, coupled with layer by layer technology, allows us to obtain highly aqueous stable Platicur nanocolloids of about 100 nm. The visible light-activated Platicur nanocolloids showed an increased drug release and antitumor activity on HeLa cells, with respect to Platicur nanocolloids in darkness. This occurrence could give very interesting insight into selective activation of the nanodelivered Pt(II) complex and possible side-effect lowering. For the first time, the metabolic effects of Platicur nanocolloid photoactivation, in the HeLa cell line, have been investigated using an NMR-based metabolomics approach coupled with statistical multivariate data analysis. The reported results highlight specific metabolic differences between photoactivated and non-photoactivated Platicur NC-treated HeLa cancer cells.

Chronic Oleoylethanolamide Treatment Decreases Hepatic Triacylglycerol Level in Rat Liver by a PPARγ/SREBP-Mediated Suppression of Fatty Acid and Triacylglycerol Synthesis

“…The acquisition and processing of spectra were performed using Topspin 3.5 software (Bruker Biospin, Milan, Italy). Resonances of fatty acids and metabolites were assigned according with data available in the literature [38][39][40].…”

Low-Intensity Light-Responsive Anticancer Activity of Platinum(II) Complex Nanocolloids on 2D and 3D In Vitro Cancer Cell Model

“…Nanocolloids were built up by sonication assisted layer-by-layer technique [ 38 ]. Biodegradable chitosan (CHI, polycation) and pectin (PEC, polyanion) were chosen for a biocompatible and biodegradable coating on drug nanoparticles.…”

A NMR-Based Metabolomic Approach to Investigate the Antitumor Effects of the Novel [Pt(η1-C2H4OMe)(DMSO)(phen)]+ (phen = 1,10-Phenanthroline) Compound on Neuroblastoma Cancer Cells

“…e preparation of samples was carefully carried out following a specific protocol in order to minimize the possible NMR chemical shift variations in the subsequent analysis, according to reported literature [26][27][28]31].…”

“…In the last few years, NMR-based metabolomics has been extended to the study of metal-based antitumor drugs for assessing mode of action and specific targets in cancer cells [24,25]. e application of NMR spectroscopy in this research field opened new perspectives for the study of new potential drugs by providing a metabolic fingerprint of biological systems and simplifying preclinical trials [24][25][26][27][28].…”