Visible Diode Laser Enhancement of Exotic DNA Uptake by Fowl Sperm

doi:10.7324/jabb.2015.3407

Cited by 1 publication

(1 citation statement)

References 28 publications

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“…The genes in this network encode for proteins involved in lipid metabolism, e.g., carboxyl ester lipase (CEL) secreted from the pancreas to breakdown cholesterol and lipid-soluble vitamin ester hydrolysis and absorption, and protein metabolism. Also, carboxypeptidase, which hydrolyzes a C-terminal peptide bond in polypeptide chains, signal proteins involved in cell differentiation, proliferation, and photoreceptor proteins that convert the light waves into signals, e.g., light-absorbing chromophores (Karu, 1996;Shichida and Matsuyama, 2009;El-Gendy et al, 2015;de Freitas and Hamblin, 2016;Losi et al, 2018). The activity in pancreatic tissue is mostly upregulated, which is consistent with the need for enzymes to metabolize the fat and protein from egg yolk to support the embryogenesis process.…”

Section: Overrepresented Gene Ontology and Pathway Terms Indicate Lighting Stimulates Early Life Metabolic Activitymentioning

confidence: 69%

Effects of Monochromatic Lighting During Incubation and Vaccination on the Splenic Transcriptome Profiles of Chicken

et al. 2021

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Lighting is a crucial environmental variable in poultry operations, but illumination during incubation is relatively understudied. The ability to stimulate development or immune performance using in ovo lighting is a promising approach for improving poultry health and welfare. This study investigated how monochromatic green light during incubation and vaccination method and timing affected chicken splenic gene expression patterns. We performed this study with 1,728 Hy-Line white layer eggs incubated under two light treatments during incubation: continuous dark and continuous green monochromatic light, over the entire incubation period. Half the eggs in each light treatment received in ovo vaccination, applied on embryonic day 18 (ED18). The remaining half were vaccinated by spraying on hatch day. After hatching, the light treatments followed the industry-standard lighting regimens. The study had six treatment groups with light–dark pairs for non-vaccinated, in ovo vaccinated, and post-hatch vaccinated. We assessed splenic gene expression at ED18 and at 7 days post-hatch (PH) in all the treatments. We isolated and sequenced 24 mRNA libraries on the Illumina platform, followed by bioinformatics and differential gene expression analyses. RNAseq analysis showed between 62 and 6,755 differentially expressed genes (DEGs) between comparisons, with the most prominent differences observed between ED and PH samples, followed by comparisons between vaccination methods. In contrast, light vs. dark treatments at ED showed limited effects on transcriptomic profiles. However, we observed a synergistic effect of lighting during incubation on post-hatch vaccination responses, with differentially expressed genes (DEGs) unique to the light treatment showing stimulation of cell proliferation with significance for immune activity (inferred from gene ontology terms). Gene ontology and pathway analysis indicated biological processes like cellular component organization or biogenesis, rhythmic process, developmental process, response to stimulus, and immune system processes were explained by the DEGs. While lighting is an important source of circadian stimulation, other controlled studies are required to clarify whether in ovo circadian entrainment plays a role in modulating immune responses.

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