Visible continuum pulses based on enhanced dispersive wave generation for endogenous fluorescence imaging

Quan, Cui; Chen, Zhongyun; Liu, Qian; Luo, Qingming; Fu, Ling

doi:10.1364/boe.8.004026

Cited by 8 publications

(3 citation statements)

References 30 publications

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“…The flat dispersion with a low value, small effective mode area, high nonlinear coefficient, and low loss are all essential factors in improving SCG efficiency, which has been verified in HPCFs infiltrated with toluene (C 7 H 8 ), chloroform (CHCl 3 ), benzene (C 6 H 6 ), nitrobenzene (C 6 H 5 NO 2 ), and tetrachloroethylene (C 2 Cl 4 ) [2][3][4][5][6][7][8][9] . The outstanding advantages of SCGs include the further spectral expansion in the near-infrared region, high-coherent spectrum, smoothness, low noise, and flat-top, making them have a wide range of applications in the fields of optical communication, fiber optic sensing, optical coherence tomography, optical metrology and spectroscopy [10][11][12][13][14] . The continuous development of fiber optic technology and the introduction of new materials are endless sources of inspiration for researchers in the design of novel optical fibers.…”

Section: Introductionmentioning

confidence: 99%

A study on the optical properties of a novel flower-core photonic crystal fiber infiltrated with CCl4

Nguyen

2023

Sci. Tech. Dev. J.

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A novel design of photonic crystal fiber with a flower-core is proposed for the first time to optimize dispersion suitable for broadband supercontinuum generation. The numerical simulation of the propagation of light modes in optical fibers is computed by solving Maxwell's wave equations with the full-vector finite-difference eigenmode method. As a result, near-zero ultra-flat all-normal and anomalous dispersion was achieved with fluctuations of ±0.978 ps/nm.km in the 294 nm wavelength region and ±1.168 ps/nm.km in the 432 nm wavelength region, respectively. Furthermore, the dispersion value at the pump wavelength is about 10 times lower than in previous papers. The combination of CCl4 infiltration into the hollow core and the air hole's size heterogeneity in the cladding also enhances the nonlinear properties of the fibers. The effective mode areas less than 2 µm2 at the pump wavelength can help the supercontinuum spectrum to broaden further towards the red wavelength region. Three fibers with structural parameters Ʌ = 0.8 µm, d1/Ʌ = 0.45, Ʌ = 0.9 µm, d1/Ʌ = 0.5, and Ʌ = 0.9 µm, d1/Ʌ = 0.45 are proposed for supercontinuum generation with low peak power due to the nonlinear coefficients as high as hundreds W−1.km−1. These fibers are a new class of optical fibers, highly efficient laser sources that replace traditional glass-core optical fibers.

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Section: Introductionmentioning

confidence: 99%

A study on the optical properties of a novel flower-core photonic crystal fiber infiltrated with CCl4

Nguyen

2023

Sci. Tech. Dev. J.

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“…More confidential excitation solutions based on a supercontinuum laser system (SCLS) have existed in MPM for 15 years (Cui et al, 2017; Eibl et al, 2018; Li, Zheng, & Qu, 2009; Magnol, Blanquet, & Lefort, 2018; Palero, Boer, Vijverberg, Gerritsen, & Sterenborg, 2005; Poudel & Kaminski, 2019; Tada et al, 2007; Tao, Bao, & Gu, 2011; Tu et al, 2016). Such solutions are ideal for multiplexing the excitation beam thus resulting in a so called instrument of “multiplex‐multiphoton microscopy” (M‐MPM) system.…”

Section: Introductionmentioning

confidence: 99%

Multiplex‐multiphoton microscopy and computational strategy for biomedical imaging

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Carrion

Chouzenoux

et al. 2021

Microscopy Res & Technique

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We demonstrate the benefit of a novel laser strategy in multiphoton microscopy (MPM). The cheap, simple, and turn‐key supercontinuum laser system with its spectral shaping module, constitutes an ideal approach for the one‐shot microscopic imaging of many fluorophores without modification of the excitation parameters: central wavelength, spectral bandwidth, and average power. The polyvalence of the resulting multiplex‐multiphoton microscopy (M‐MPM) device is illustrated by images of many biomedical models from several origins (biological, medical, or vegetal), generated while keeping constant the spectral parameters of excitation. The resolution of the M‐MPM device is quantified by a procedure of point‐spread‐function (PSF) assessment led by an original, robust, and reliable computational approach FIGARO. The estimated values for the PSF width for our M‐MPM system are shown to be comparable to standard values found in optical microscopy. The simplification of the excitation system constitutes a significant instrumental progress in biomedical MPM, paving the way to the imaging of many fluorophores with a single shot of excitation without any modification of the lighting device. Research Highlights A new solution of multiplex‐multiphoton microscopy device is shown, resting on a supercontinuum laser. The one‐shot excitation device has imaged biomedical and vegetal models. Our original computational strategy measures usual microscopy resolution.

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“…The compatibility of an ultra-wideband laser system (UWLS) with MPM has been highlighted about ten years ago [17][18][19][20][21][22][23]. This solution consists usually in the spectral broadening of femtosecond laser pulses into a photonics crystal fiber (PCF) generating frequencies compatible with multiphoton excitation in the NIR.…”

Section: Introductionmentioning

confidence: 99%

Advanced biomedical multiphoton fluorescence microscopy with a large band excitation system

Hortholary

Carrion

Lefort

2020

Biomedical Spectroscopy, Microscopy, and Imaging

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Commercial multiphoton microscopes currently include standard and bulk titanium-sapphire laser sources (Ti: Sa) delivering a narrow spectral bandwidth of 10 nm at the full-width at half maximum. Such a spectral width precludes the instantaneous imaging of more than four fluorophores simultaneously in optimal conditions of target. Femtosecond ultrawideband laser system (UWLS) appeared since fifteen years with an approach of spectral broadening of Ti: Sa pulses into a photonic crystal fiber associated with a dispersion compensation system. In the current work, we demonstrate the interest of an alternative laser solution, compact, cheap, simple and turn-key based on a UWLS delivering a unique spectral excitation bandwidth named supercontinuum. We have coupled this system to a passive filtering setup. Thanks to this unique spectral bandwidth, eleven fluorophores are imaged with multiphoton processes without any modification on the excitation parameters. .

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Visible continuum pulses based on enhanced dispersive wave generation for endogenous fluorescence imaging

Cited by 8 publications

References 30 publications

A study on the optical properties of a novel flower-core photonic crystal fiber infiltrated with CCl4

A study on the optical properties of a novel flower-core photonic crystal fiber infiltrated with CCl4

Multiplex‐multiphoton microscopy and computational strategy for biomedical imaging

Advanced biomedical multiphoton fluorescence microscopy with a large band excitation system

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