Visfatin Stimulates Proliferation of MCF-7 Human Breast Cancer Cells

Abstract

Obesity, a condition characterized by increased fat content and altered secretion of adipokines, is a risk factor for postmenopausal breast cancer. Visfatin has recently been established as a novel adipokine that is highly enriched in visceral fat. Here we report that visfatin regulated proliferation of MCF-7 human breast cancer cells. Exogenous administration of recombinant visfatin increased cell proliferation and DNA synthesis rate in MCF-7 cells. Furthermore, visfatin activated G1-S phase cell cycle progression by upregulation of cyclin D1 and cdk2 expression. Visfatin also increased the expression of matrix metalloproteinases 2, matrix metalloproteinases 9, and vascular endothelial growth factor genes, suggesting that it may function in metastasis and angiogenesis of breast cancer. Taken together, these findings suggest that visfatin plays an important role in breast cancer progression.

Possible Role of Visfatin in Hepatoma Progression and the Effects of Branched-Chain Amino Acids on Visfatin-Induced Proliferation in Human Hepatoma Cells

“…Serum visfatin level is a good biomarker of colorectal malignant potential and stage progression (19). Visfatin stimulation increases cell proliferation in prostate and breast cancer cells (20,21), whereas the use of visfatin inhibitor exerts an antitumor effect by inducing apoptosis (22). These findings suggest that visfatin is one of the key adipocytokines that links obesity and tumorigenesis and thus may be an effective target for the inhibition of obesity-related carcinogenesis.…”

Effects of Apelin-13 on Human Prostate Cancer Lines [Insan Prostat Kanseri Hucre Serilerinde Apelin-13'un Etkileri]

“…Obezitenin bazı kanser formlarıyla olan ilişkisi uzun zamandır bilinmekte [49] ve obezite ile kanser arasındaki önemli bağlantılardan birinin anormal adipokin salgısı olduğu ileri sürülmektedir [50]. Mevcut literatürlerde yağ doku kaynaklı bazı hormonların kanser ile ilişkisini açıklayan çalışmalar bulunmaktadır [51][52][53][54]. Yağ doku kaynaklı bir hormon olan visfatinin, MCF-7 hücre hattı üzerinde yapılan denemeleri sonucu bu hormonun doz bağımlı olarak hücre canlılığını arttığı gösterilmiştir [51].…”

“…Mevcut literatürlerde yağ doku kaynaklı bazı hormonların kanser ile ilişkisini açıklayan çalışmalar bulunmaktadır [51][52][53][54]. Yağ doku kaynaklı bir hormon olan visfatinin, MCF-7 hücre hattı üzerinde yapılan denemeleri sonucu bu hormonun doz bağımlı olarak hücre canlılığını arttığı gösterilmiştir [51]. Catalano ve ark.…”

NAMPT (nicotinamide phosphoribosyltransferase)

“…Additionally, Nampt is present in bovine mammary epithelium, lactating mammary glands, and milk (Yonezawa et al, 2006). NAMPT stimulated the proliferation and DNA synthesis rate of MCF-7 human BC cells (Kim et al, 2010). More specifically, NAMPT upregulated mRNA levels of cyclin D1 and cdk2, well-known regulators for the G1-S progression (Kim et al, 2010).…”

“…NAMPT stimulated the proliferation and DNA synthesis rate of MCF-7 human BC cells (Kim et al, 2010). More specifically, NAMPT upregulated mRNA levels of cyclin D1 and cdk2, well-known regulators for the G1-S progression (Kim et al, 2010). Circulating levels of Nampt were significantly elevated in women suffering from postmenopausal BC than in controls independently from known risk factors of BC, anthropometric and metabolic parameters as well as serum concentrations of leptin and adiponectin (Dalamaga et al, 2011).…”