Viscosity measurements using a two-photon ratiometric fluorescent sensor with two rotors

Liu, Fei; Luo, Yeshen; Xu, Meiying

doi:10.1016/j.tetlet.2018.11.009

Cited by 19 publications

(7 citation statements)

References 46 publications

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“…Xu and colleagues reported a two‐photon ratiometric fluorescent sensor 7 for viscosity determination [34] . Probe 7 was composed of hemicyanine and carbazole with two free rotation sites.…”

Section: Small Molecular Fluorescent Probes For Viscositymentioning

confidence: 99%

Small Molecular Fluorescent Probes for Imaging of Viscosity in Living Biosystems

Xiao

Tang

2021

Chemistry A European J

102

116

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Viscosity, as a vital microenvironment parameter, is tightly associated with multitudinous cellular processes and diseases. Recently, precise visualization of viscosity has started to arouse more and more interest. However, owing to the complicated character, it is still a huge challenge to directly observe viscosity in living systems. In this regard, mounting fluorescence probes are being increasingly fabricated to map viscosity inside live cells and small animals. In this minireview, the viscosity‐sensitive small molecular fluorescent probes used in bioimaging are comprehensively summarized, mainly focusing on the last three years. Moreover, the current challenges and opportunities for the development of viscosity‐specific fluorescent probes will be discussed.

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Section: Small Molecular Fluorescent Probes For Viscositymentioning

confidence: 99%

Small Molecular Fluorescent Probes for Imaging of Viscosity in Living Biosystems

Xiao

Tang

2021

Chemistry A European J

102

116

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“…In the case of HeLa cells, these studies reported heterogeneous and relatively high viscosity values between 20.7 and 275 cP (Yang et al, 2013;J ımenez-S anchez et al, 2018;Ren et al, 2018Ren et al, , 2020Steinmark et al, 2019). Similarly, variable high mitochondrial viscosity values between < 100 and > 900 cP were reported in rat hepatic tissue slices (Liu et al, 2018), whereas COS7 cells displayed a viscosity value of 325 cP, measured with a BODIPY-HaloTag rotor molecule targeted to mitochondria using the mitochondrial Cox8a targeting sequence (Chambers et al, 2018). It is currently unknown why the rotor-based viscosity values exceed those reported in the current study and earlier FP-based research (Partikian et al, 1998;Verkman, 2002;Koopman et al, 2007Koopman et al, , 2008bDieteren et al, 2011a).…”

Section: Discussionmentioning

confidence: 90%

Stress‐dependent macromolecular crowding in the mitochondrial matrix

et al. 2023

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Macromolecules of various sizes induce crowding of the cellular environment. This crowding impacts on biochemical reactions by increasing solvent viscosity, decreasing the water‐accessible volume and altering protein shape, function, and interactions. Although mitochondria represent highly protein‐rich organelles, most of these proteins are somehow immobilized. Therefore, whether the mitochondrial matrix solvent exhibits macromolecular crowding is still unclear. Here, we demonstrate that fluorescent protein fusion peptides (AcGFP1 concatemers) in the mitochondrial matrix of HeLa cells display an elongated molecular structure and that their diffusion constant decreases with increasing molecular weight in a manner typical of macromolecular crowding. Chloramphenicol (CAP) treatment impaired mitochondrial function and reduced the number of cristae without triggering mitochondrial orthodox‐to‐condensed transition or a mitochondrial unfolded protein response. CAP‐treated cells displayed progressive concatemer immobilization with increasing molecular weight and an eightfold matrix viscosity increase, compatible with increased macromolecular crowding. These results establish that the matrix solvent exhibits macromolecular crowding in functional and dysfunctional mitochondria. Therefore, changes in matrix crowding likely affect matrix biochemical reactions in a manner depending on the molecular weight of the involved crowders and reactants.

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“…On the contrary, with the increase of the viscosity, an increase in fluorescence intensity can be observed since the energy absorbed decays as a fluorescence rather than being dissipated as kinetic energy. The peculiar optical behavior of such molecular rotors have been widely used to analyze the viscosity of fluids and have found application in several research fields ranging from the quality control of silicone oils for mechanical engineering [ 32 ] to the characterization of the viscosity of cell's compartments and living tissues in biology [ 33 ]. Thioflavin T (Tht) is a fluorescent molecular rotor commonly used to image amyloids [ 34 ].…”

Section: Introductionmentioning

confidence: 99%

Thioflavin-modified molecularly imprinted hydrogel for fluorescent-based non-enzymatic glucose detection in wound exudate

Giovannini

Cinelli

Boesel

et al. 2022

Materials Today Bio

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Viscosity measurements using a two-photon ratiometric fluorescent sensor with two rotors

Cited by 19 publications

References 46 publications

Small Molecular Fluorescent Probes for Imaging of Viscosity in Living Biosystems

Small Molecular Fluorescent Probes for Imaging of Viscosity in Living Biosystems

Stress‐dependent macromolecular crowding in the mitochondrial matrix

Thioflavin-modified molecularly imprinted hydrogel for fluorescent-based non-enzymatic glucose detection in wound exudate

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