Viscose cellulose sponge as an implantable matrix: Changes in the structure increase the production of granulation tissue

Pajulo, Q; Viljanto, J; Lönnberg, B.; Hurme, Timo; Lönnqvist, K; Saukko, Pekka

doi:10.1002/(sici)1097-4636(199611)32:3<439::aid-jbm18>3.0.co;2-b

Cited by 26 publications

(13 citation statements)

References 6 publications

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“…The speed of the connective tissue ingrowth not only depends on the size, shape and internal structure of the implant, but on the age, sex and species of the experimental animal as well [23]. In our case, the implants had a macroporous structure, which permitted the easy access of the host tissue infiltration at the subcutaneous epigastric groin fascia of young male Wistar rats.…”

Section: Discussionmentioning

confidence: 67%

In VitroandIn VivoDegradation of Oxidized Acetyl- and Ethyl-Cellulose Sponges

Elçin

2006

Artificial Cells, Blood Substitutes, and Biotechnology

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The objective of this study was to assess the in vitro and in vivo degradation properties of macroporous sponges composed of oxidized acetyl-cellulose (AC; 45.000 Mw) and ethyl-cellulose (EC; 50.000 Mw). The sponges were constructed by solvent-casting and particulate-leaching technique using a polymer concentration of 2.5 and 5.0% (w:v), and periodate oxidation. The resulting sponges were: AC2.5, AC5.0, EC2.5 and EC5.0. While AC sponges exhibited a gradual degradation overtime, EC sponges had a very slow in vitro mass loss. In general, sponges made up of 2.5% (w:v) polymer content degraded faster than the ones with 5.0% (w:v). The sponges degraded faster at pH 5.0, compared to pH 6.0 and 7.4 conditions. About 60%, 44% and 31% of dry mass loss was determined for AC2.5 sponges after 60 weeks at pH 5.0, pH 6.0 and pH 7.4 conditions, respectively; thus, ca. 21%, 13% and 12% of dry mass loss from EC2.5 sponges was observed at the same pH conditions, in the same order. The in vivo degradation studies were performed on Wistar rats (n = 24) for a duration of 60 weeks. In general, all sponge implants were well-tolerated by the subjects. While granulation tissue or fibrotic capsule was not formed around the sponges, neovascularization was observed. AC and EC sponges demonstrated an in vivo degradation behavior quite similar to that observed for the in vitro study conducted at pH 5.0 conditions. Histomorphometric analysis revealed that the in vivo degradation of AC2.5 and EC2.5 after 60 weeks was about 47% and 18%, respectively. The results indicate that oxidized acetyl cellulose may be considered as a partially degradable scaffold material for tissue engineering applications.

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Section: Discussionmentioning

confidence: 67%

In VitroandIn VivoDegradation of Oxidized Acetyl- and Ethyl-Cellulose Sponges

Elçin

2006

Artificial Cells, Blood Substitutes, and Biotechnology

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“…Several studies report the applicability of cellulose-based materials for culturing cells and for implantation. [2][3][4][5] Cellulose is the most abundant naturally occurring polymer on earth produced by plants and bacteria that has been utilized in applications from paper and textiles to materials in wound healing. Cellulose can be processed into a range of forms such as membrane sponges, microspheres, nonwovens, and knitted textiles.…”

Section: ' Introductionmentioning

confidence: 99%

Biomimetic Calcium Phosphate Crystal Mineralization on Electrospun Cellulose-Based Scaffolds

Rodríguez¹,

Renneckar²,

Gatenholm

2011

ACS Appl. Mater. Interfaces

160

100

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Novel cellulose based-scaffolds were studied for their ability to nucleate bioactive calcium phosphate crystals for future bone healing applications. Cellulose-based scaffolds were produced by electrospinning cellulose acetate (CA) dissolved in a mixture of acetone/dimethylacetamide (DMAc). The resulting nonwoven CA mats containing fibrils with diameters in the range of 200 nm to 1.5 μm were saponified by NaOH/ethanol for varying times to produce regenerated cellulose scaffolds. Biomimetic crystal growth nucleated from the fiber surface was studied as a function of surface chemistry. Regenerated cellulose scaffolds of varying treatments were soaked in simulated body fluid (SBF) solution. Scaffolds that were treated with CaCl(2), a mixture of carboxymethyl cellulose (CMC) and CaCl(2), and NaOH and CaCl(2), were analyzed using X-ray photoelectron spectroscopy, X-ray powder diffraction, and scanning electron microscopy to understand the growth of bioactive calcium phosphate (Ca-P) crystals as a function of surface treatment. The crystal structure of the nucleated Ca-P crystals had a diffraction pattern similar to that of hydroxyapatite, the mineralized component of bone. The study shows that the scaffold surface chemistry can be manipulated, providing numerous routes to engineer cellulosic substrates for the requirements of scaffolding.

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“…These techniques have been used to measure interactions of cellulose surfaces [4][5][6][7][8][9] aiming mostly to increase the insight into interfacial phenomena in traditional cellulose applications such as papermaking. With growing interest in new cellulose applications often involving biomolecules or even cells and tissues (drug delivery devices [10], affinity separation [11], tissue engineering [12]) surface force measurements are becoming important to advance understanding of interfacial phenomena in such complex multi-component systems based on cellulose polymers.…”

Section: Introductionmentioning

confidence: 99%

Atomic force microscopy study of cellulose surface interaction controlled by cellulose binding domains

Nigmatullin

Lovitt

Wright

et al. 2004

Colloids and Surfaces B: Biointerfaces

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Colloidal probe microscopy has been used to study the interaction between model cellulose surfaces and the role of cellulose binding domain (CBD), peptides specifically binding to cellulose, in interfacial interaction of cellulose surfaces modified with CBDs.The interaction between pure cellulose surfaces in aqueous electrolyte solution is dominated by double layer repulsive forces with the range and magnitude of the net force dependent on electrolyte concentration. AFM imaging reveals agglomeration of CBD adsorbed on cellulose surface. Despite an increase in surface charge owing to CBD binding to cellulose surface, force profiles are less repulsive for interactions involving, at least, one modified surface. Such changes are attributed to irregularity of the topography of protein surface and non-uniform distribution of surface charges on the surface of modified cellulose.Binding double CBD hybrid protein to cellulose surfaces causes adhesive forces at retraction, whereas separation curves obtained with cellulose modified with single CBD show small adhesion only at high ionic strength. This is possibly caused by the formation of the cross-links between cellulose surfaces in the case of double CBD.

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Viscose cellulose sponge as an implantable matrix: Changes in the structure increase the production of granulation tissue

Cited by 26 publications

References 6 publications

In VitroandIn VivoDegradation of Oxidized Acetyl- and Ethyl-Cellulose Sponges

In VitroandIn VivoDegradation of Oxidized Acetyl- and Ethyl-Cellulose Sponges

Biomimetic Calcium Phosphate Crystal Mineralization on Electrospun Cellulose-Based Scaffolds

Atomic force microscopy study of cellulose surface interaction controlled by cellulose binding domains

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