Viscoll collagen solution as a novel bioink for direct 3D bioprinting

Osidak, E. O.; Каралкин, П. А.; Osidak, M. S.; Parfenov, Vladislav A.; Sivogrivov, D. E.; Pereira, Frederico D. A. S.; Gryadunova, A. A.; Koudan, Elizaveta V.; Khesuani, Yusef D.; Kasyanov, Vladimir; Belousov, Sergei I.; Krasheninnikov, Sergey V.; Grigoriev, T. E.; Чвалун, С. Н.; Буланова, Елена А.; Domogatsky, S.P.

doi:10.1007/s10856-019-6233-y

Cited by 104 publications

(96 citation statements)

References 48 publications

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“…The shear thinning and viscosity behavior of high concentration neutralized Col (2-6% w/v) has been characterized by different groups [20,31]. In contrast, 3D printing of neutralized pure Col at low concentrations is challenging due to the lack of shear thinning and shape retention.…”

Section: Discussionmentioning

confidence: 99%

“…Neutralized non fibrillar Col (0.8% w/v) was held at low temperature before extrusion and gelation was induced by printing at 37°C [19]. In another study, neutralized Col fractions were printed at high concentrations (2-4% w/v) and lower temperature (4-15°C) during extrusion printing and gelled within pre warmed media [20]. However, none of these studies investigated the formation and presence of Col fibers within the printed hydrogel construct.…”

Section: Introductionmentioning

confidence: 99%

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Tissue mimetic hyaluronan bioink containing collagen fibers with controlled orientation modulating cell morphology and alignment

Schwab

Hélary

Richards

et al. 2020

Preprint

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Biofabrication is providing scientists and clinicians the ability to produce engineered tissues with desired shapes, chemical and biological gradients. Typical resolutions achieved with extrusion-based bioprinting are at the macroscopic level. However, for capturing the fibrillar nature of the extracellular matrix (ECM), it is necessary to arrange ECM components at smaller scales, down to the sub-micron and the molecular level. In this study, we introduce a (bio)ink containing hyaluronan (HA) as tyramine derivative (THA) and collagen (Col). Similarly to other connective tissues, in this (bio)ink Col is present in fibrillar form and HA as viscoelastic space filler. THA was enzymatically crosslinked under mild conditions allowing simultaneous Col fibrillogenesis, thus achieving a homogeneous distribution of Col fibrils within the viscoelastic HA-based matrix. THA-Col composite displayed synergistic properties in terms of storage modulus and shear-thinning, translating into good printability. Shear-induced alignment of the Col fibrils along the printing direction was achieved and quantified via immunofluorescence and second harmonic generation. Cell-free and cell-laden constructs were printed and characterized, analyzing the influence of the controlled microscopic anisotropy on cell behavior and chondrogenic differentiation. THA-Col showed cell instructive properties modulating hMSC adhesion, morphology and sprouting from spheroids stimulated by the presence and the orientation of Col fibers. Actin filament staining showed that hMSCs embedded into aligned constructs displayed increased cytoskeleton alignment along the fibril direction. Based on gene expression of cartilage/bone markers and matrix production, hMSCs embedded into the bioink displayed chondrogenic differentiation comparable or superior to standard pellet culture by means of proteoglycan production (Safranin O staining and proteoglycan quantification) as well as increase in cartilage related genes. The possibility of printing matrix components with control over microscopic alignment brings biofabrication one step closer to capturing the complexity of native tissues.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Tissue mimetic hyaluronan bioink containing collagen fibers with controlled orientation modulating cell morphology and alignment

Schwab

Hélary

Richards

et al. 2020

Preprint

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“…Additional crosslinking of collagen molecules can be used to enhance the mechanical properties of collagen to provide structural integrity for additive bio-manufacturing such as for muscle tissue (8-20 kPa), cartilage tissue (20-30 kPa) and bone tissue (2-30 GPa) [45,47]. These "artificial" crosslinking bonds can be generated using chemical agents or physical treatment.…”

Section: Methods Of Collagen Crosslinkingmentioning

confidence: 99%

“…Following the extrusion process, scaffolds self-assembled in a neutral buffer to support self-assembly. This process produced tissue spheroid scaffolds as well as printing cell-laden inks into pre-set extrusion designs [47,112].…”

Section: Extrusionmentioning

confidence: 99%

Additive Biomanufacturing with Collagen Inks

et al. 2020

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Collagen is a natural polymer found abundantly in the extracellular matrix (ECM). It is easily extracted from a variety of sources and exhibits excellent biological properties such as biocompatibility and weak antigenicity. Additionally, different processes allow control of physical and chemical properties such as mechanical stiffness, viscosity and biodegradability. Moreover, various additive biomanufacturing technology has enabled layer-by-layer construction of complex structures to support biological function. Additive biomanufacturing has expanded the use of collagen biomaterial in various regenerative medicine and disease modelling application (e.g., skin, bone and cornea). Currently, regulatory hurdles in translating collagen biomaterials still remain. Additive biomanufacturing may help to overcome such hurdles commercializing collagen biomaterials and fulfill its potential for biomedicine.

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Section: Hybrid Fabrication: Specific Case Analyses and Emerging Key mentioning

confidence: 99%

Advances in Hybrid Fabrication toward Hierarchical Tissue Constructs

et al. 2020

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The diversity of manufacturing processes used to fabricate 3D implants, scaffolds, and tissue constructs is continuously increasing. This growing number of different applicable fabrication technologies include electrospinning, melt electrowriting, volumetric‐, extrusion‐, and laser‐based bioprinting, the Kenzan method, and magnetic and acoustic levitational bioassembly, to name a few. Each of these fabrication technologies feature specific advantages and limitations, so that a combination of different approaches opens new and otherwise unreachable opportunities for the fabrication of hierarchical cell–material constructs. Ongoing challenges such as vascularization, limited volume, and repeatability of tissue constructs at the resolution required to mimic natural tissue is most likely greater than what one manufacturing technology can overcome. Therefore, the combination of at least two different manufacturing technologies is seen as a clear and necessary emerging trend, especially within biofabrication. This hybrid approach allows more complex mechanics and discrete biomimetic structures to address mechanotransduction and chemotactic/haptotactic cues. Pioneering milestone papers in hybrid fabrication for biomedical purposes are presented and recent trends toward future manufacturing platforms are analyzed.

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Viscoll collagen solution as a novel bioink for direct 3D bioprinting

Cited by 104 publications

References 48 publications

Tissue mimetic hyaluronan bioink containing collagen fibers with controlled orientation modulating cell morphology and alignment

Tissue mimetic hyaluronan bioink containing collagen fibers with controlled orientation modulating cell morphology and alignment

Additive Biomanufacturing with Collagen Inks

Advances in Hybrid Fabrication toward Hierarchical Tissue Constructs

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